Inhibitor analysis and adaptive evolution of Saccharomyces cerevisiae for simultaneous saccharification and ethanol fermentation from industrial waste corncob residues

Gu, Hanqi; Zhang, Jian; Bao, Jie

doi:10.1016/j.biortech.2014.01.060

Cited by 69 publications

(56 citation statements)

References 32 publications

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“…The major components of glucose, xylose, arabinose, acetic acid, and furans, as well as a wide variety of aliphatic acid compounds can be detected directly from hydrolysate samples by highperformance liquid chromatography (HPLC) or gas chromatography (GC) using different columns and detectors (Franden et al 2013;Gu et al 2014). Minor components, especially for phenolic compounds, have been extracted from hydrolysate using organic solvents, concentrated by evaporation, and analyzed by gas chromatographymass spectrometer (GC-MS), liquid chromatographymass spectrometer (LC-MS), and/or inductively coupled plasma mass spectrometer (ICP-MS) (Gu et al 2014;Wang et al 2014). Inductively coupled plasma optical emission spectrometry (ICP-OES) has also been used for inorganic ion analysis and many different mineral elements such as magnesium, potassium, manganese, iron, copper, and calcium have been identified in lignocellulosic hydrolysates (Jin et al 2013;Le et al 2014).…”

Section: Identification and Quantification Of Inhibitors In Lignocellmentioning

confidence: 99%

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Yang

Tang

et al. 2018

Bioresour. Bioprocess.

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Pretreatment is the key step to overcome the recalcitrance of lignocellulosic biomass making sugars available for subsequent enzymatic hydrolysis and microbial fermentation. During the process of pretreatment and enzymatic hydrolysis as well as fermentation, various toxic compounds may be generated with strong inhibition on cell growth and the metabolic capacity of fermenting strains. Zymomonas mobilis is a natural ethanologenic bacterium with many desirable industrial characteristics, but it can also be severely affected by lignocellulosic hydrolysate inhibitors. In this review, analytical methods to identify and quantify potential inhibitory compounds generated during lignocellulose pretreatment and enzymatic hydrolysis were discussed. The effect of hydrolysate inhibitors on Z. mobilis was also summarized as well as corresponding approaches especially the high-throughput ones for the evaluation. Then the strategies to enhance inhibitor tolerance of Z. mobilis were presented, which include both forward and reverse genetics approaches such as classical and novel mutagenesis approaches, adaptive laboratory evolution, as well as genetic and metabolic engineering. Moreover, this review provided perspectives and guidelines for future developments of robust strains for efficient bioethanol or biochemical production from lignocellulosic materials.

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Section: Identification and Quantification Of Inhibitors In Lignocellmentioning

confidence: 99%

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Yang

Tang

et al. 2018

Bioresour. Bioprocess.

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“…The total phenolic content in the whole slurries of the hydrolysate was measured with the modified Folin and Ciocalteu method (Ainsworth and Gillespie, 2007;Gu et al, 2014).…”

Section: Analysis Of Sugars Ethanol Inhibitors and Total Phenolic Cmentioning

confidence: 99%

“…The hydrolysate contained 98.16 ± 0.70 g/L of glucose and 6.49 ± 0.79 g/L of xylose. A four-step adaptation procedure of the angel yeast using the hydrolysate was conducted according to Gu et al (2014) before the yeast culture was used as the seed of ethanol fermentation.…”

Section: Strain and Short-term Adaptationmentioning

confidence: 99%

“…Among various lignocellulose feedstocks for cellulosic ethanol production, corncob residue shows advantages in its high cellulose content and no pretreatment requirement (Liu et al, 2010;Fang et al, 2010;Zhang et al, 2010a;Fan et al, 2013;Gu et al, 2014). Currently, the corncob biorefinery industry for productions of xylose, xylo-oligomers, xylitol, or furfural has firmly established in China .…”

Section: Introductionmentioning

confidence: 99%

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An alternative feedstock of corn meal for industrial fuel ethanol production: Delignified corncob residue

Liu

Zhang

Xiao³

et al. 2014

Bioresource Technology

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“…However, many laboratory yeast strains are more vulnerable to inhibition in the real hydrolysates of fermentation processes compared with industrial yeast due to the high content of inhibitors (Hou and Yao 2011;Zhu et al 2015;Dubey et al 2016). To improve the tolerance of yeast strains to inhibitors, adaptation was widely applied to improve the strain tolerance (Hawkins and Doran-Peterson 2011;Wang et al 2013;Gu et al 2014). However, the adapted strains were usually unstable and susceptible to degeneration.…”

Section: Introductionmentioning

confidence: 99%

Hybridization Improves Inhibitor Tolerance of Xylose-fermenting Saccharomyces cerevisiae

Li¹,

Zhu²,

Li³

et al. 2017

BioResources

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Although some engineered S. cerevisiae strains exhibit good xylose utilization ability, the lack of tolerance to inhibitors generated in biomass pretreatment limits the application of such strains in the production of bioethanol from lignocellulosic biomass. By applying a sexual mating method, inhibitor tolerance was developed in xylose-utilizing strains. The final ethanol concentrations in simultaneous scarification and cofermentation (SScF) process at 38 °C with hybrid strains were 50% higher than the SScF process with the xylose-fermenting parent strain. The strain viability of the hybrid strain E7-12 at 24 h was 282 times higher than the parent strain in the SScF process at 25% solid loading. Due to the improved sugar utilization, the final ethanol concentration reached 69.7 g/L (E7-11) and 70.0 g/L (E7-12), which were 25.3 g/L and 25.6 g/L higher than that of SScF with the xylose-fermenting strain, respectively.

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Inhibitor analysis and adaptive evolution of Saccharomyces cerevisiae for simultaneous saccharification and ethanol fermentation from industrial waste corncob residues

Cited by 69 publications

References 32 publications

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

An alternative feedstock of corn meal for industrial fuel ethanol production: Delignified corncob residue

Hybridization Improves Inhibitor Tolerance of Xylose-fermenting Saccharomyces cerevisiae

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