Inhibitor activities of chickpeas (<i>Cicer arietinum</i> L) against bovine, pocine and human trypsin and chymotrypsin

Saini, H.S.; Weder, Jürgen K. P.; Knights, E. J.

doi:10.1002/jsfa.2740600304

Cited by 20 publications

(17 citation statements)

References 24 publications

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“…These discrepancies could be due to the presence of certain amounts of antinutritional factors in the seed, which can vary considerably among batches of the same legume. Saini et al (1992) observed a large variation in concentrations of trypsin and chymotrypsin inhibitors of chickpea grown in Australia, which were influenced by the location and year of cultivation. Singh and Jambunathan (1981) also showed that trypsin inhibitor activity of two varieties of chickpea varied considerably among different genotypes.…”

Section: Discussionmentioning

confidence: 99%

Nutritional value of raw and extruded chickpeas (Cicer arietinum L.) for growing chickens

Brenes

Viveros²,

Centeno³

et al. 2008

Span. j. agric. res.

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The effects of the inclusion of different concentrations (0, 100, 200 and 300 g kg -1 ) of raw and extruded chickpeas on performance, digestive organ sizes, and protein and fat digestibilities were studied in one experiment with growing broiler chickens (0 to 21 days of age). Data were analyzed as a 3 x 2 factorial arrangement with three levels of chickpea with or without extrusion. A corn-soybean based diet was used as a positive control. Increasing chickpea content in the diet did not affect weight gain, feed consumption and feed to gain ratio. Relative pancreas and liver weights, and relative lengths of duodenum, jejunum and ceca were significantly (P<0.05) increased in response to increasing chickpea concentration in the diet. The inclusion of graded concentrations of chickpea increased (P<0.05) the apparent ileal digestibility (AID) of crude protein (CP) and apparent excreta digestibility (AED) of crude fat (CF) only in the case of the intermediate level of chickpea used (200 g kg -1 ). Extrusion improved weight gain and lowered relative pancreas weight (P< 0.05) respect to birds fed raw chickpea-based diets. AID of CP and AED of CF were improved (P<0.001) by extrusion. We concluded that the inclusion of up to 300 g kg -1 chickpea in chicken diets did not affect performance, and caused a negative effect on the relative weight of some digestive organs.Additional key words: chick, extrusion, legumes. ResumenValor nutritivo del garbanzo crudo y extrusionado en pollos de aptitud cárnica Se realizó un experimento con el objeto de estudiar el efecto de la inclusión de distintas concentraciones (0, 100, 200 y 300 g kg -1 ) de garbanzo crudo y extrusionado sobre los parámetros productivos, el peso y la longitud de los órganos digestivos y la digestibilidad de la proteína y la grasa de pollos broiler (0-21 días de edad). Los datos fueron analizados siguiendo un diseño factorial (3 x 2) con tres concentraciones de garbanzo con o sin extrusión. Se utilizó una dieta basada en maíz-soja como control positivo sin garbanzo. La inclusión de cantidades crecientes de garbanzo en la dieta no modificó la ganancia de peso, el consumo de alimento ni el índice de transformación de las aves, pero sí los pesos relativos del pán-creas e hígado y las longitudes relativas del duodeno, yeyuno, íleon y ciego que se incrementaron significativamente (P<0,05). La digestibilidad aparente ileal (AID) de la proteína bruta (CP) y la digestibilidad aparente fecal (AED) de la grasa bruta (CF) se incrementaron (P<0,05) sólo en el caso de la incorporación de 200 g kg -1 de garbanzo. La extrusión mejoró la ganancia de peso de las aves, la AID (P<0,001) de la CP y la AED de la CF y disminuyó (P<0,05) el peso relativo del páncreas. En conclusión, la inclusión de hasta 300 g kg -1 de garbanzo no produjo modificaciones en los paráme-tros productivos de las aves y causó efectos negativos en algunos órganos digestivos.Palabras clave adicionales: aves, extrusión, leguminosas.Abbreviations used: AED (apparent excreta digestibility), AIA (acid insol...

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Section: Discussionmentioning

confidence: 99%

Nutritional value of raw and extruded chickpeas (Cicer arietinum L.) for growing chickens

Brenes

Viveros²,

Centeno³

et al. 2008

Span. j. agric. res.

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“…No inhibitory activity was detected for porcine and human chymotrypsin, but the crude extract was able to weakly inhibit bovine quimotrypsin. A large number of grain legumes have been shown to inhibit trypsin and chymotrypsin from several animal sources (3,7,8).…”

Section: Discussionmentioning

confidence: 99%

“…Assays of trypsin and chymotrypsin inhibition were determined by using BAPNA 4 and BTPNA as synthetic substrates, respectively, according to the published procedure (7,8). Inhibition of trypsin and chymotrypsin by the crude extract was assessed by incubating the enzymes with diluted extracts; the results were expressed as milligrams of active enzyme completely inhibited by extracts from 1.0 g of seeds.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterization of a New Trypsin Inhibitor from Crotalaria paulina Seeds

Pando

Ciero²,

Novello

et al. 1999

IUBMB Life

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SummaryA new trypsin inhibitor (CPTI) has been isolated from Crotalaria paulina seeds. Puri cation of the inhibitor was carried out by gel ltration, ion-exchange chromatography, and subsequent reversed-phase HPLC. The presence of a single polypeptide chain, with a molecular mass of 20 kDa and isoelectric point 4.0, was detected. The trypsin inhibitor had a K i value of 4.5 £ 10 ¡ 8 M and was capable of acting on human, bovine, and porcine trypsin and weakly on bovine chymotrypsin. Amino acid analysis showed that CPTI has a high content of aspartate, glutamate, leucine, serine, and glycine, having 177 amino acid residues in its composition. These data suggest that the protein belongs to the Kunitz-type trypsin inhibitors. IUBMB Life, 48: 519-523, 1999

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“…Studies on the accumulation of Pi during chickpea seed development and the interaction of these Pi with HGP are, therefore, important. Although biochemical properties of mature chickpea seed Pi and their nutritional significance have been studied in detail by several workers (Belew et al, 1975;Belew and Eaker, 1976;Smirnoff et al, 1976Smirnoff et al, , 1979Jibson et al, 1981;Sastry and Murray, 1987;Saini et al, 1992), the biological properties and significance of Pi during seed development have not been reported.…”

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Chickpea Defensive Proteinase Inhibitors Can Be Inactivated by Podborer Gut Proteinases1

et al. 1998

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Developing chickpea (Cicer arietinum L.) seeds 12 to 60 d after flowering (DAF) were analyzed for proteinase inhibitor (Pi) activity. In addition, the electrophoretic profiles of trypsin inhibitor (Ti) accumulation were determined using a gel-radiographic filmcontact print method. There was a progressive increase in Pi activity throughout seed development, whereas the synthesis of other proteins was low from 12 to 36 DAF and increased from 36 to 60 DAF. Seven different Ti bands were present in seeds at 36 DAF, the time of maximum podborer (Helicoverpa armigera) attack. Chickpea Pis showed differential inhibitory activity against trypsin, chymotrypsin, H. armigera gut proteinases, and bacterial proteinase(s). In vitro proteolysis of chickpea Ti-1 with various proteinases generated Ti-5 as the major fragment, whereas Ti-6 and -7 were not produced. The amount of Pi activity increased severalfold when seeds were injured by H. armigera feeding. In vitro and in vivo proteolysis of the earlyand late-stage-specific Tis indicated that the chickpea Pis were prone to proteolytic digestion by H. armigera gut proteinases. These data suggest that survival of H. armigera on chickpea may result from the production of inhibitor-insensitive proteinases and by secretion of proteinases that digest chickpea Pis.In a co-evolving system of plant-insect interactions, plants synthesize a variety of toxic proteinaceous and nonproteinaceous molecules for their protection against insects

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Inhibitor activities of chickpeas (Cicer arietinum L) against bovine, pocine and human trypsin and chymotrypsin

Cited by 20 publications

References 24 publications

Nutritional value of raw and extruded chickpeas (Cicer arietinum L.) for growing chickens

Nutritional value of raw and extruded chickpeas (Cicer arietinum L.) for growing chickens

Isolation and Characterization of a New Trypsin Inhibitor from Crotalaria paulina Seeds

Chickpea Defensive Proteinase Inhibitors Can Be Inactivated by Podborer Gut Proteinases1

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