Inhibiton of photodynamic haemolysis byGratiolaofficinalisL.extract

Tkachenko, N. V.; Pravdin, Alexander B.; Terentyuk, George; Navolokin, Nikita; Kurchatova, M. N.; Polukonova, N. V.

doi:10.1117/12.2179862

Cited by 7 publications

(5 citation statements)

References 5 publications

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“…Ранее на лабораторных животных с перевиваемыми опухолями было показано, что флавоноидсодержащий экстракт аврана лекарственного (Gratiola officinalis L.) наряду с малой токсичностью обладает противоопухолевой активностью в отношении перевиваемого рака печени и саркомы, а также антиоксидантным и антикахексическим свойством [5][6][7]. Однако детального изучения апоптоза, аутофагии и механизмов гибели опухолевых клеток перевиваемого рака почки с применением иммуногистохимических маркеров под действием экстракта аврана ранее не проводилось.…”

Section: Original Investigationsunclassified

The expression of apoptosis and autophagy markers in transplantable rat kidney cancer with the administration of flavonoid-containing hedge hyssop ( L.) extract

et al. 2019

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Цель исследования-в экспериментах in vivo установить механизмы патоморфоза перевитого рака почки под влиянием флавоноидсодержащего экстракта аврана. Материал и методы. Эксперимент проводили на 30 самцах крыс Wistar c перевитым раком почки РА. В опытных группах крысы через 72 ч после перевивки опухоли на протяжении 12 сут получали экстракт аврана перорально или внутримышечно в дозе 110 мг/кг/сут. Группу сравнения составили животные с опухолью, но без воздействия. Использовали иммуногистохимические маркеры апоптоза (p53, bax, bcl-2, Fas-receptor, Fas-ligand), аутофагии LС3b, пролиферации Ki-67 и ангиогенеза VEGF. При статистической обработке данных нормальность распределения показателей в группах проверяли при помощи критерия Шапиро-Уилка. Для сравнения групп использовали критерий Крамера-Уэлча (Т). Результаты. При гистологическом исследовании в ткани опухоли под действием экстракта аврана наблюдали появление обширных зон повреждения (некроз и апоптозные тельца). При обоих путях введения экстракта аврана отмечали резкое снижение экспрессии маркеров пролиферации (Ki-67), ангиогенеза (VEGF) и высокую экспрессию маркеров апоптоза (p53, bax, CD95 (Fas/APO-1), FAS-ligand) в опухолевых клетках и отсутствие ее в группе сравнения. Все описанные изменения были более выражены при внутримышечном введении. Экспрессия маркера аутофагии LC3B увеличивалась при пероральном и уменьшалась при внутримышечном введении экстракта аврана. Заключение. Под влиянием экстракта аврана развивается выраженный патоморфоз рака почки. Происходят подавление пролиферации, ангиогенеза и активация сигнального и митохондриального пути апоптоза, а также блокирование протекторной аутофагии. Маркер аутофагии LC3b может использоваться в качестве дополнительного критерия оценки лечебного патоморфоза опухолей.

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Section: Original Investigationsunclassified

The expression of apoptosis and autophagy markers in transplantable rat kidney cancer with the administration of flavonoid-containing hedge hyssop ( L.) extract

et al. 2019

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“…Previously, we have developed a method for separation of flavonoid-containing extracts from Gratīola officinālis L. that allows obtaining non-toxic or low toxic extracts even from poisonous plants [5,6]. It is know that these extract have antioxidant [7,8], antitumor [9][10][11][12][13], antimicrobial and antiinflammatory [14,15] actions.…”

Section: Introductionmentioning

confidence: 99%

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

Polukonova¹,

Navolokin²,

Bucharskaya³

et al. 2018

Russ Open Med J

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Objective -The discovery of the apoptosis-inducing effects of flavonoid vagonin allowed to make an assumption of existence of similar effect in others flavonoids. This study is devoted to the effects of Gratīola officinālis extract on cell culture of the human kidney cancer.Methods -Cell cultures of human kidney carcinoma -Caki-1 and SN12c were used in the study. The cells were stained with Hoechat 33258 dye. The number of living cells, cells in a state of apoptosis and mitosis were counted. The Cramer-Welch criterion (T) was used to compare the obtained data.Results -The activation of apoptosis was noted at all concentrations of the Gratiola officinalis L. extract during the first day of exposure. The apoptotic activity increased with increasing of extract concentration. After 48 hours, this activity was maintained only at a Gratiola officinalis L. extract concentration of 0.9 mg/ml. After 24 hours, the apoptotic activity of the extract was more expressed in the culture of CaKi-1. However, after 48 hours the extract induces more pronounced apoptosis in the culture of Sn12c cells. The cytotoxic activity of the extract was not differ after 24 hours in both cultures, after 48 hours it was more pronounced in the culture of CaKi-1. Conclusion -We revealed a pronounced antitumor and apoptotic activity of the Gratiola officinalis L. extract against the cultures of the kidney cancer Caki-1 and Sn12c. Apoptosis of tumor cells can be manifested in the form of pycnosis of the nucleus, the formation of apoptotic bodies and the emergence of cellular debris resulting from complete degradation of tumor cells. The apoptotic activity of the Gratiola officinalis extract depends on their concentration.

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“…One of the representatives of the flavonoid-containing extracts is the herbal extract from the Gratiola officinalis L. Different methods of extraction from Gratiola officinalis make it possible to obtain biologically active compositions with various pharmacological effects: laxative, vomiting, antispasmodic, diuretic, digitalis-like effect on the heart, antioxidant, antitumor and immunomodulatory, antipyretic, antimicrobial, antituberculosis [19][20][21]. The flavonoid containing extract obtained by the authors' method is not toxic and able to positively influence the state of red bone marrow [22,23].…”

Section: Introductionmentioning

confidence: 99%

Wound Healing Activity Of Extract Of Gratiola Officinalis In Vivo

et al. 2021

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Background and aim — Gratiola officinalis extract has antimicrobial properties, but its wound healing activity has not been described. Material and Methods — The aqueous solution of dry Gratiola officinalis extract was used for treatment of skin wounds with different routes of administration. In the experiment 40 white laboratory male rats with weight of 150±50 grams were subjected to the incised model of flat skin wounds in the interscapular region under the general anesthesia. A morphological study was carried out to assess the effectiveness of the therapy administered. Results — The most effective method of treatment was found to be local application of the extract, which reduced wound healing time by 8 days. Intramuscular and oral administrations reduce healing time by 6 days. Morphological signs of regeneration, which developed under the influence of Gratiola officinalis extract, allowed to estimate the wound healing activity. Clear differentiation and thickness of layers of the newly formed epidermis, a large number of thin-walled vessels and actively proliferating fibroblasts indicate an active regeneration process. Conclusion — This extract may be interesting to specialists in regenerative medicine as a remedy for treatment of wound processes.

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Inhibiton of photodynamic haemolysis byGratiolaofficinalisL.extract

Cited by 7 publications

References 5 publications

The expression of apoptosis and autophagy markers in transplantable rat kidney cancer with the administration of flavonoid-containing hedge hyssop ( L.) extract

The expression of apoptosis and autophagy markers in transplantable rat kidney cancer with the administration of flavonoid-containing hedge hyssop ( L.) extract

The apoptotic activity of flavonoid-containing Gratiola officinalis extract in cell cultures of human kidney cancer

Wound Healing Activity Of Extract Of Gratiola Officinalis In Vivo

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