Inhibitive Effects of Mulberry Leaf-Related Extracts on Cell Adhesion and Inflammatory Response in Human Aortic Endothelial Cells

Abstract

Effects of mulberry leaf-related extracts (MLREs) on hydrogen peroxide-induced DNA damage in human lymphocytes and on inflammatory signaling pathways in human aortic endothelial cells (HAECs) were studied. The tested MLREs were rich in flavonols, especially bombyx faces tea (BT) in quercetin and kaempferol. Polyphenols, flavonoids, and anthocyanidin also abounded in BT. The best trolox equivalent antioxidant capacity (TEAC) was generated from the acidic methanolic extracts of BT. Acidic methanolic and water extracts of mulberry leaf tea (MT), mulberry leaf (M), and BT significantly inhibited DNA oxidative damage to lymphocytes based on the comet assay as compared to the H2O2-treated group. TNF-α-induced monocyte-endothelial cell adhesion was significantly suppressed by MLREs. Additionally, nuclear factor kappa B (NF-κB) expression was significantly reduced by BT and MT. Significant reductions were also observed in both NF-κB and activator protein (AP)-1 DNA binding by MLREs. Significant increases in peroxisome proliferator-activated receptor (PPAR) α and γ DNA binding by MLREs were also detected in M and MT extracts, but no evidence for PPAR α DNA binding in 50 μg/mL MT extract was found. Apparently, MLREs can provide distinct cytoprotective mechanisms that may contribute to its putative beneficial effects on suppressing endothelial responses to cytokines during inflammation.

Kaempferol enhances endothelium‐dependent relaxation in the porcine coronary artery through activation of large‐conductance Ca2+‐activated K+ channels

“…Kaempferol is a flavonoid present in many plants (including tea, grapes and mulberry) (Olszewska, ; Chao et al ., ; Nile et al ., ). It is also one of the major bioactive components of the Chinese herbal medicine Carthamus tinctorius (Fan et al ., ) used for the management of various cardiovascular disorders including thrombosis, hypertension and coronary artery disease.…”

Antioxidant Activities of Methanol Extracts from Selected Taiwanese Herbaceous Plants

“…Moreover, acidic methanolic extracts of MA and Houttuynia cordata (HC) significantly suppressed oxidative damage to lymphocyte DNA [15]. Over the past decade, we have evaluated more than 50 edible plant samples representing highly consumed and under-utilized plant species using Trolox equivalent antioxidant capacity (TEAC) and 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity methods to investigate the antioxidant activity of their water-and methanol-soluble antioxidants in plant extracts [14][15][16][17][18][19].…”

Medicinal Plants as a Potential and Successful Treatment Option in the Context of Atherosclerosis

“…Mulberry leaf extract protects lymphocyte DNA from oxidative damage, prevents ROS-mediated endothelial cell dysfunction via downregulating intracellular redox-dependent signaling pathways. It also has anti-inflammatory effects through the modulation of AP-1, NF-kB, PPARs, and STAT3 signaling (Chao et al, 2013).…”