Inhibition Studies with 2-Bromoethanesulfonate Reveal a Novel Syntrophic Relationship in Anaerobic Oleate Degradation

Salvador, A. F.; Cavaleiro, A. J.; Paulo, Artur Cavaco; Silva, S. A.; Guedes, Ana P.; Pereira, M. A.; Stams, Alfons Johannes Maria; Sousa, Diana Z.; Alves, M. M.

doi:10.1128/aem.01733-18

Cited by 34 publications

(26 citation statements)

References 49 publications

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“…High-throughput sequencing (16S rRNA gene) by Illumina MiSeq technology were performed at the RTL Genomics (Lubbock, TX). Detailed description of the procedure was previously described (Salvador et al, 2019). All the samples were analyzed in duplicate.…”

Section: Microbiota Analysismentioning

confidence: 99%

In vitro assessment of prebiotic properties of xylooligosaccharides produced by Bacillus subtilis 3610

Amorim

Silvério

Cardoso

et al. 2020

Carbohydrate Polymers

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Xylooligosaccharides (XOS) are emergent prebiotics exhibiting high potential as food ingredients. In this work, in vitro studies were performed using human fecal inocula from two healthy donors (D 1 and D2) to evaluate the prebiotic effect of commercial lactulose and XOS produced in a single-step by recombinant Bacillus subtilis 3610. The fermentation of lactulose led to the highest production of lactate (D1: 33.7 ± 0.5 mM; D2:19.7 ± 0.3 mM) and acetate (D1: 77.5 ± 0.6 mM; D2: 81.0 ± 0.7 mM), while XOS led to the highest production of butyrate (D1: 9.0 ± 0.6 mM; D2: 10.5 ± 0.8 mM) and CO 2 (D1: 8.92 ± 0.02 mM; D2: 11.4 ± 0.3 mM). Microbiota analysis showed a significant decrease in the relative abundance of Proteobacteria for both substrates and an increase in Bifidobacterium and Lactobacillus for lactulose, and Bacteroides for XOS.

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Section: Microbiota Analysismentioning

confidence: 99%

In vitro assessment of prebiotic properties of xylooligosaccharides produced by Bacillus subtilis 3610

Amorim

Silvério

Cardoso

et al. 2020

Carbohydrate Polymers

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“…Considering that Fe(II) and methane were produced concomitantly with close-to-stoichiometric acetate accumulation in the IR assays ( Figure 2 c,e), oleate oxidation was most likely performed by syntrophic bacteria in close relationship with both hydrogen-consuming IRB and methanogens. LCFA conversion by syntrophic bacteria and sulfate- or sulfonate-reducing hydrogen-consuming bacteria was previously reported by Sousa et al [ 36 ] and Salvador et al [ 31 ], respectively, but syntrophic interaction between LCFA-degrading bacteria and IRB was not described before. Dissimilatory Fe(III) reduction proceeded at a faster rate than hydrogenotrophic methanogenesis ( Figure 2 c,e), which possibly represented an advantage for the syntrophic relationships, thus accelerating oleate oxidation.…”

Section: Discussionmentioning

confidence: 87%

“…Samples were amplified for sequencing using the universal primer pair 515f and 806r [ 30 ], targeting the prokaryotic 16S rRNA gene. Details on the sequencing and bioinformatics data analysis can be found elsewhere [ 31 ]. Nucleotide sequences were submitted to the European Nucleotide Archive (ENA) under the accession numbers ERS3774021 to ERS3774023, associated with the study number PRJEB33626.…”

Section: Methodsmentioning

confidence: 99%

Effect of Sub-Stoichiometric Fe(III) Amounts on LCFA Degradation by Methanogenic Communities

et al. 2020

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Long-chain fatty acids (LCFA) are common contaminants in municipal and industrial wastewater that can be converted anaerobically to methane. A low hydrogen partial pressure is required for LCFA degradation by anaerobic bacteria, requiring the establishment of syntrophic relationships with hydrogenotrophic methanogens. However, high LCFA loads can inhibit methanogens, hindering biodegradation. Because it has been suggested that anaerobic degradation of these compounds may be enhanced by the presence of alternative electron acceptors, such as iron, we investigated the effect of sub-stoichiometric amounts of Fe(III) on oleate (C18:1 LCFA) degradation by suspended and granular methanogenic sludge. Fe(III) accelerated oleate biodegradation and hydrogenotrophic methanogenesis in the assays with suspended sludge, with H2-consuming methanogens coexisting with iron-reducing bacteria. On the other hand, acetoclastic methanogenesis was delayed by Fe(III). These effects were less evident with granular sludge, possibly due to its higher initial methanogenic activity relative to suspended sludge. Enrichments with close-to-stoichiometric amounts of Fe(III) resulted in a microbial community mainly composed of Geobacter, Syntrophomonas, and Methanobacterium genera, with relative abundances of 83–89%, 3–6%, and 0.2–10%, respectively. In these enrichments, oleate was biodegraded to acetate and coupled to iron-reduction and methane production, revealing novel microbial interactions between syntrophic LCFA-degrading bacteria, iron-reducing bacteria, and methanogens.

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“…Liquid samples from fecal inocula and fermentation broth (48 h) were taken and used for DNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, Solon, OH), according to the manufacturer's procedures. The extracted DNA samples were further submitted to highthroughput sequencing (16S rRNA gene) by Illumina MiSeq technology, as described in detail previously (Salvador et al, 2019). All the sequencing analysis were performed at RTL Genomics (Lubbock, TX) and all the samples were analyzed in duplicate.…”

Section: Microbial Analysismentioning

confidence: 99%

In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient

Amorim

Silvério

Cardoso

et al. 2020

LWT

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Until now the prebiotic potential of pure trisaccharide raffinose on human health assessed through highthroughput sequencing remains poorly investigated. In this work, an in vitro model using human fecal inocula of two healthy volunteers (D1 and D2) was used to study the prebiotic potential of raffinose and compare it with the well-stablished and commercial prebiotic lactulose. The intestinal microbiota showed preference for raffinose as substrate showing the highest consumption value at 48 h (96.0 ± 0.9% D1 and 95.3 ± 0.7% D2). The fermentation of raffinose decreased the medium pH, the ammonia concentration and the relative amount of Proteobacteria, while increasing the total production of lactate and short chain fatty acids (129.9 ± 2.6 mmol/L D1 and 179.6 ± 0.6 mmol/L D2), CO 2 (10.8 ± 0.8 mmol/L medium D1 and 5.2 ± 0.3 mmol/L medium D2) and the relative amount of Bifidobacterium and Lactobacillus. This study suggests that raffinose holds potential functional properties for human health.

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Inhibition Studies with 2-Bromoethanesulfonate Reveal a Novel Syntrophic Relationship in Anaerobic Oleate Degradation

Cited by 34 publications

References 49 publications

In vitro assessment of prebiotic properties of xylooligosaccharides produced by Bacillus subtilis 3610

In vitro assessment of prebiotic properties of xylooligosaccharides produced by Bacillus subtilis 3610

Effect of Sub-Stoichiometric Fe(III) Amounts on LCFA Degradation by Methanogenic Communities

In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient

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