“…The use of HPLC in isolation of fruit volatiles is not useful for the total analysis of the complex mixture of pear volatiles, but has proven to be a ready tool to identify and quantify some important volatile compounds of pear aroma, such as decadienoates (Russell et al 1981;Quamme 1984), a-farnesene, and conjugated trienes (Zoffoli et al 1998). Indeed, these compounds are characterized by a relatively high degree of unsaturation (Crombie 1955), and thus can be spectrophotometrically detected and quantified by HPLC with an UV detector between 200 and 300 nm (Jennings and Creveling 1963;Heinz et al 1964;Creveling and Jennings 1970;Quamme and Marriage 1977;Chen et al 1990;Chen et al 1993). HPLC, in contrast to GC, prevents thermal generation of artifacts (Kubeczka 1981).…”