Inhibition of α- and β-Hydroxysteroid Dehydrogenases and Steroid Δ-Isomerase by Substrate Analogues¹

Inhibition of human-type 1 3β-hydroxysteroid deshydrogenase/Δ5-Δ4-isomerase expression using siRNA

“…This dsRNA form of mRNA then becomes a substrate for Dicer cleavage activity, which leads to the destruction of the mRNA [6]. Although many chemical inhibitors of 3␤-HSD activity have been described [7][8][9][10][11], specific inhibition of type 1 3␤-HSD could not yet be found because of the high homology between 3␤-HSD types 1 and 2. In this report, we describe the use of DNA vector-based RNAi approach, which has the potential to specifically destroy high-homology mRNAs to inhibit type 1 3␤-HSD.…”

Mitochondrial Damage in Experimental Congenital Adrenal Hyperplasia

“…Neither the 3a-or I7ß-steroid dehydrogenase nor the 11or 21-hydroxylase is affected (Goldman and Winter, 1967) . However, in vitro inhibition of bovine and Pseudomonas testosteroni A 5-3-ketosteroid isomerase and steroid 3a-dehydrogenase has been demonstrated a ; Neville and Engel, 1968) .…”