Inhibition of xanthine oxidase by liquiritigenin and isoliquiritigenin isolated from Sinofranchetia chinensis

Abstract

The methanol extract of the stem of Sinofranchetia inhibited the activity of xanthine oxidase in vitro. Bioassay-guided purification led to the isolation ofliquiritigenin and isoliquiritigenin as the main xanthine oxidase inhibitors. This inhibition of enzyme activity was found to be dose dependent, with an IC50 value of approximately 49.3 microM for liquiritigenin and 55.8 microM for isoliquiritigenin. Lineweaver-Burk transformation of the inhibition data indicated that the inhibition was of a mixed type for both liquiritigenin and isoliquiritigenin. For liquiritigenin, the Ki and K(I) were determined to be 14.0 microM and 151.6 microM, respectively. For isoliquiritigenin, the Ki and K(I) were determined to be 17.4 microM and 81.9 microM, respectively. These results suggest that these natural products could be used to treat conditions where the inhibition of xanthine oxidase is warranted.

Inhibition of xanthine oxidase by Caulerpenyne from Caulerpa prolifera

“…The well-known commercial XOD inhibitor is allopurinol. Although it has been used in clinical applications largely, the use of it can cause severe side effects such as hypersensitivity syndrome, Stevens Johnson syndrome, renal toxicity, allergy and skin problems [7][8][9]. Thus, there is a need to develop new XOD inhibitors which are safer and more effective than the currently used drugs.…”

“…Thus, there is a need to develop new XOD inhibitors which are safer and more effective than the currently used drugs. It might be possible to use XOD inhibitors for the treatment of hepatitis and brain tumor where it was determined that the activity of XOD is increased [8,10]. Moreover, it was reported that XOD has a significant role in many diseases such as cardiovascular disorders, inflammations, chronic heart failure [3].…”

“…There are a lot publications which evaluate the potential of terrestrial plants as XOD inhibitors [7][8][9][11][12][13][14]. The present study evaluates the potential of caulerpenyne (CYN) as a XOD inhibitor.…”

Enhanced Detection and Structural Characterization of Flavonoids by Complexation with N,O-Bis(trimethysilyl)trifluoroacetamide Using Electrospray Ionization Mass Spectrometry

“…1 The flavonoids have aroused considerable interest recently because of their potential beneficial effects on human health; they have been reported to be antioxidants, antimicrobials, free radical scavengers, and metal chelators, and to have anti-allergic, anti-inflammatory, anti-tumor, anti-viral, and anti-bacterial properties. [2][3][4][5][6][7][8][9][10][11][12][13][14][15] Since flavonoids are an ubiquitous part of the human diet, their effects on human health are of interest. The study of health effects of flavonoids demands that sensitive methods be developed for their determination and quantifications.…”

Isolamento e identificação de compostos com atividade antibacteriana da própolis vermelha brasileira

“…Por exemplo, este composto exibiu atividade no músculo cardíaco (WEGENER et al, 1997), atividade antioxidante (PRACHAYASITTIKUL et al, 2008), efeito inibitório da xantina oxidase (KONG et al, 2000) e tirosinase (ZHENG et al, 2008), além de inibir a proliferação e induzir a apoptose em hepatoma humano (HSU et al, 2005). Também diminuiu a produção de prostaglandinas e óxido nítrico em macrófagos de rato (TAKAHASHI et al, 2004) e apresentou uma potente atividade inibitória de células cancerígenas de próstata (JUNG et al, 2006).…”