Inhibition of voltage-gated sodium channels by bisphenol A in mouse dorsal root ganglion neurons

Wang, Qiang; Cao, Jie; Zhu, Qin; Luan, Chunye; Chen, Xiao; Yi, Xiaohua; Ding, Hu; Chen, Jian; Cheng, Jie; Xiao, Hang

doi:10.1016/j.brainres.2011.01.022

Cited by 36 publications

(32 citation statements)

References 32 publications

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“…Interestingly, T-glase and Nylon 680 significantly hyperpolarized PPN neurons. This may be because compounds in either of these plastics activate some variety of potassium channels or alter other channel conductances (Asano et al, 2010; Rottgen et al, 2014; Wang et al, 2011). This may also be because the cells are healthier in the presence of these two plastics.…”

Section: Discussionmentioning

confidence: 99%

The use of three-dimensional printing to produce in vitro slice chambers

Hyde

MacNicol

Odle

et al. 2014

Journal of Neuroscience Methods

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Background In recent years, 3D printing technology has become inexpensive and simple enough that any lab can own and use one of these printers. New Method We explored the potential use of 3D printers for quickly and easily producing in vitro slice chambers for patch clamp electrophysiology. Slice chambers were produced using five available plastics: ABS, PLA, Nylon 618, Nylon 680, and T-glase. These “lab-made” chambers were also made using stereolithography through a professional printing service (Shapeways). This study measured intrinsic membrane properties of neurons in the brain stem pedunculopontine nucleus (PPN) and layer V pyramidal neurons in retrosplenial cortex. Results Nylon 680 and T-glase significantly hyperpolarized PPN neurons. ABS increased input resistance, decreased action potential amplitude, and increased firing frequency in pyramidal cortical neurons. To test long term exposure to each plastic, human neuroblastoma SHSY5Y cell cultures were exposed to each plastic for 1 week. ABS decreased cell counts while Nylon 618 and Shapeways plastics eliminated cells. Primary mouse pituitary cultures were also tested for 24-hour exposure. ABS decreased cell counts while Nylon 618 and Shapeways plastics decreased cell counts. Comparison to Existing Methods Chambers can be quickly and inexpensively printed in the lab. ABS, PLA, Nylon 680, and T-glase plastics would suffice for many experiments instead of commercially produced slice chambers. Conclusions While these technologies are still in their infancy, they represent a powerful addition to the lab environment. With careful selection of print material, slice chambers can be quickly and inexpensively manufactured in the lab.

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Section: Discussionmentioning

confidence: 99%

The use of three-dimensional printing to produce in vitro slice chambers

Hyde

MacNicol

Odle

et al. 2014

Journal of Neuroscience Methods

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“…BPA was shown to affect voltage-gated sodium currents in dissociated dorsal root ganglion neurons [40]. However, these studies did not investigate BPA action in a defined heterologous expression system or identify a specific binding site.…”

Section: Discussionmentioning

confidence: 99%

Bisphenol A Binds to the Local Anesthetic Receptor Site to Block the Human Cardiac Sodium Channel

et al. 2012

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Bisphenol A (BPA) has attracted considerable public attention as it leaches from plastic used in food containers, is detectable in human fluids and recent epidemiologic studies link BPA exposure with diseases including cardiovascular disorders. As heart-toxicity may derive from modified cardiac electrophysiology, we investigated the interaction between BPA and hNav1.5, the predominant voltage-gated sodium channel subtype expressed in the human heart. Electrophysiology studies of heterologously-expressed hNav1.5 determined that BPA blocks the channel with a Kd of 25.4±1.3 µM. By comparing the effects of BPA and the local anesthetic mexiletine on wild type hNav1.5 and the F1760A mutant, we demonstrate that both compounds share an overlapping binding site. With a key binding determinant thus identified, an homology model of hNav1.5 was generated based on the recently-reported crystal structure of the bacterial voltage-gated sodium channel NavAb. Docking predictions position both ligands in a cavity delimited by F1760 and contiguous with the DIII–IV pore fenestration. Steered molecular dynamics simulations used to assess routes of ligand ingress indicate that the DIII–IV pore fenestration is a viable access pathway. Therefore BPA block of the human heart sodium channel involves the local anesthetic receptor and both BPA and mexiletine may enter the closed-state pore via membrane-located side fenestrations.

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“…12 Patch pipettes were pulled from thin-walled borosilicate glass on a vertical puller (PP-830; Narishige, Japan). The resistance between the recording electrode filled with the pipette solution and the reference electrode was 2-5 M. Capacity transients were cancelled as much as possible, and voltage errors were reduced by 80-90% by series resistance compensation.…”

Section: Whole Cell Patch-clamp Recordingmentioning

confidence: 99%

Capsazepine concentration dependently inhibits currents in HEK 293 cells mediated by human hyperpolarization-activated cyclic nucleotide-gated 2 and 4 channels

Zuo

Zhang

et al. 2013

Exp Biol Med (Maywood)

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Recent studies indicate that blockade of currents (Ih) mediated by hyperpolarization-activated cyclic nucleotide-gated (HCN) channels (particularly HCN1) may partly account for the antinociceptive effects of capsazepine (CPZ). Unfortunately, determining whether capsazepine is a selective HCN channel blocker and determining its adverse effects when it is used for the treatment of neuropathic pain, have been thus far understudied. In this study, we aimed to elucidate the effects of capsazepine on human HCN2 (hHCN2) and HCN4 (hHCN4) channels in HEK293 cells. The vectors that expressed hHCN2 and hHCN4 cDNA were constructed and transfected into HEK293 cells. Enhanced green fluorescent protein (EGFP) fluorescence and the reverse transcription polymerase chain reaction (RT-PCR) were used to confirm the successful transfection of the vectors. After G418 (neomycin) screening, cell lines that expressed hHCN2 and hHCN4 were obtained. The whole-cell voltage-clamp technique was used to determine the currents from hHCN2 and hHCN4 channels, which were perfused with five concentrations (0.1 µM, 1 µM, 5 µM, 10 µM and 50 µM) of capsazepine. The results showed that capsazepine at the range from 0.1 to 50 µM markedly inhibited hHCN2 and hHCN4 currents in a concentration-dependent manner, with most inhibition achieved at a concentration of 10 µM of capsazepine. When compared with the control group, a V0.5 for the hHCN2 and hHCN4 channel showed that 10 µM capsazepine significantly shifted the membrane potential towards hyperpolarization. The present results indicate that capsazepine is not a selective HCN1 channel blocker and that it may have adverse effects when used to treat neuropathic pain.

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Inhibition of voltage-gated sodium channels by bisphenol A in mouse dorsal root ganglion neurons

Cited by 36 publications

References 32 publications

The use of three-dimensional printing to produce in vitro slice chambers

The use of three-dimensional printing to produce in vitro slice chambers

Bisphenol A Binds to the Local Anesthetic Receptor Site to Block the Human Cardiac Sodium Channel

Capsazepine concentration dependently inhibits currents in HEK 293 cells mediated by human hyperpolarization-activated cyclic nucleotide-gated 2 and 4 channels

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