Inhibition of virus multiplication and alteration of cyclic AMP level in cell cultures by flavonoids

Abstract

The inhibitory effect of four flavonoid compounds on virus multiplication and their influence on the intracellular cyclic AMP (cAMP) level were studied in cell cultures. Quercetin and quercitrin reduced the yields of Human (alpha) herpesvirus 1 (HSV-1) and Suid (alpha) herpesvirus 1 (pseudorabies virus), but hesperidin and rutin had no effect. Further, quercetin and quercitrin elevated the intracellular level of cAMP, whereas hesperidin and rutin did not alter the cAMP level. Both antiviral activity and cAMP-enhancing effect were dependent on the concentrations of the flavonoids, and these effects turned out to be parallel. This study suggests that a relation exists between the antiviral effect and the cAMP-enhancing activity of flavonoids.

Flavonoid from Carica papaya inhibits NS2B-NS3 protease and prevents Dengue 2 viral assembly

“…(2011) [36], which show significant antiviral activity against DENV-2 in Vero cells. Quercetin has also been reported to have inhibitory effects on several viruses [37]. Even though p-Coumaric acid shows best binding affinity in terms of hydrogen bond it shows poor values in case of ligand-receptor atom pair interaction.…”

Propolis: chemical composition, biological properties and therapeutic activity

“…The action of these active fractions was similar both in strain spectrum and in the degree of antiinfluenza activity of propolis concentrations from 0.2-3.0 mg/ml. Amoros et al (1992aAmoros et al ( , 1992b Thiel, 1982;Ishitsuka et al, 1982;Mucsi, 1984;Mucsi and Pragai, 1985;Kaul et al, 1985;Tsuchiya et al, 1985;Vanden Berghe et al, 1986;Vrijien et al, 1988;Wleklik et al, 1988;Serkedjieva et al, 1992;Amoros et al, 1994). König and Dustmann (1985) (Scheller et al, 1977a), bone tissue and dental pulp Magro Filho and Perri de Carvalho, 1990), anaesthetic activity (Paintz and Metzner, 1979), hepatoprotective activity (Giurgea et al, 1985(Giurgea et al, , 1987Hollands et al, 1991;Tushevskii et al, 1991), increasing the number of plaque-forming cells in the spleen of populations of immunized males (Scheller et al, 1988), immunomodulatory action (Benková et al, 1989;Dimov et al, 1991Dimov et al, , 1992, immunogenic properties (Scheller et al, 1989d), liver detoxifying action, choleretic and antiulcer action in vitro (Kedzia et al, 1990), antioxidant activity (Yanishlieva and Marinova, 1986;Krol et al, 1990;Scheller et al, 1990;Dobrowolski et al, 1991;Misic et al, 1991;Olinescu, 1991;Elstner, 1993a, 1993b), anticaries in rats (Ikeno et al, 1991), protection agent against gamma irradiation in mice (Scheller et al, 1989b), antileishmaniosis in hamster (Sartori et al, 1994), antitrypanosomal agent (Higashi...…”

Antiviral activity of quercetin 7-rhamnoside against porcine epidemic diarrhea virus

“…E-mail address: dhkwon@kribb.re.kr (D.-H. Kwon). from fresh plants of H. cordata possessed direct inhibitory activity against herpes simplex virus type 1 (HSV-1), influenza virus and human immunodeficiency virus type 1 (HIV-1), without showing cytotoxicity (Lu et al, 2006). In particular, quercetin, a flavonoid present in H. cordata, has been reported to have inhibitory effects on several viruses (Mucsi and Pragai, 1985). Although a variety of pharmacological activities associated with chemicals in H. cordata has been demonstrated, antiviral activities against PEDV have not been reported.…”