Inhibition of Vibrio biofilm formation by a marine actinomycete strain A66

You, Jinhong; Xue, Xiaoli; Cao, Lixue; Lü, Xin; Wang, Jian; Zhang, Lixin; Zhou, Suhong

doi:10.1007/s00253-007-1074-x

Cited by 175 publications

(103 citation statements)

References 33 publications

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“…The potential of Actinobacteria against shrimp pathogenic Vibrio spp. made marine Actinobacteria as potential probiotic strains due to their ability to degrade macromolecules, such as starch and protein, in culture pond water; the production of antimicrobial agents; and the formation of heat-and desiccation-resistant spores [58]. Recently, a few studies were made on the possible use of marine Actinobacteria in disease prevention against aquatic pathogens.…”

Section: Probioticsmentioning

confidence: 99%

An Introduction to Actinobacteria

Ranjani¹,

Dhanasekaran²,

Gopinath³

2016

Actinobacteria - Basics and Biotechnological Applications

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Actinobacteria, which share the characteristics of both bacteria and fungi, are widely distributed in both terrestrial and aquatic ecosystems, mainly in soil, where they play an essential role in recycling refractory biomaterials by decomposing complex mixtures of polymers in dead plants and animals and fungal materials. They are considered as the biotechnologically valuable bacteria that are exploited for its secondary metabolite production. Approximately, 10,000 bioactive metabolites are produced by Actinobacteria, which is 45% of all bioactive microbial metabolites discovered. Especially Streptomyces species produce industrially important microorganisms as they are a rich source of several useful bioactive natural products with potential applications. Though it has various applications, some Actinobacteria have its own negative effect against plants, animals, and humans. On this context, this chapter summarizes the general characteristics of Actinobacteria, its habitat, systematic classification, various biotechnological applications, and negative impact on plants and animals.

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Section: Probioticsmentioning

confidence: 99%

An Introduction to Actinobacteria

Ranjani¹,

Dhanasekaran²,

Gopinath³

2016

Actinobacteria - Basics and Biotechnological Applications

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“…The biofilm assay was performed by the method described previously with further modification (You et al, 2007). Briefly, 1 ml P. aeruginosa or S. maltophilia cells, which were cultured overnight and diluted to an OD 600 of 1.0, were mixed with 200 mM of the different test compounds made up in 1 % (v/v) glycerol in 2 ml microfuge tubes that were incubated at 37 uC for 3 days without shaking.…”

Section: Methodsmentioning

confidence: 99%

Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm

Ding

Yin

et al. 2011

Journal of Medical Microbiology

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126

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The objective of this study was to screen for novel quorum-sensing inhibitors (QSIs) from traditional Chinese medicines (TCMs) that inhibit bacterial biofilm formation. Six of 46 active components found in TCMs were identified as putative QSIs based on molecular docking studies. Of these, three compounds inhibited biofilm formation by Pseudomonas aeruginosa and Stenotrophomonas maltophilia at a concentration of 200 mM. A fourth compound (emodin) significantly inhibited biofilm formation at 20 mM and induced proteolysis of the quorum-sensing signal receptor TraR in Escherichia coli at a concentration of 3-30 mM. Emodin also increased the activity of ampicillin against P. aeruginosa. Therefore, emodin might be suitable for development into an antivirulence and antibacterial agent. INTRODUCTIONPseudomonas aeruginosa, an opportunistic human pathogen, may cause acute infections in hospitalized patients, can be isolated from the environment, particularly from soil and water, and it regularly contaminates medical devices (Stover et al., 2000). It is also the predominant cause of chronic lung infection in cystic fibrosis patients (Frederiksen et al., 1997) and has recently been recognized as one of the main causes of chronic wound infections (Gjødsbøl et al., 2006). P. aeruginosa can infect patients by producing a wide range of virulence factors, the expression levels of which are tightly regulated. Key to this regulation is cell density-dependent cell-to-cell signalling, which is termed quorum sensing (QS) (Rumbaugh et al., 2000). As in many other bacteria, QS controls secretion of virulence factors (Mittal et al., 2006), biofilm formation (Waters et al., 2008) and the exchange of DNA (Fuqua & Winans, 1996) in P. aeruginosa. The biofilm mode of growth is recognized as an important bacterial trait that is relevant to infections (Costerton et al., 1994). Many infections involve the formation of bacterial biofilms, which are bacterial communities that settle and proliferate on surfaces and are covered by exopolymers (Lewis, 2007). Once established, biofilms are difficult to eradicate and become a source of secondary infection (Jones et al., 2009). Moreover, bacteria embedded in biofilms are more tolerant than planktonic cells of antibiotics (Donlan & Costerton, 2002;Drenkard, 2003). The dose of antibiotics needed in this situation will often exceed the highest deliverable dose, which makes efficient treatment impossible.QS, as a regulatory mechanism, enables bacteria to make collective decisions with respect to the expression of a specific set of genes that involve the production, release and subsequent detection of chemical signalling molecules, such as N-acylhomoserine lactones (AHLs) that are commonly used by Gram-negative bacteria. When the concentration of AHLs reaches a certain threshold level, binding to a receptor molecule (for example, LuxR) is promoted and the activated LuxR-AHL complex forms dimers or polymers, which, in turn, act as transcriptional regulators of target genes in the QS regulon (Parsek & Greenbe...

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“…The activity of C. guianensis might be due to its ability to complex with cell wall [19] and thus inhibiting the microbial growth [24]. An important step in biofilm development is the formation of the characteristic biofilm architecture [25]. Cell surface charge and CSH play a crucial role in bacterium-host cell interactions [26].…”

Section: Resultsmentioning

confidence: 99%

Impairment of the Bacterial Biofi lm Stability by Triclosan

2014

Biofilm Control and Antimicrobial Agents

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Background: Couroupita guianensis Aubl. (Lecythidaceae) is commonly called Ayahuma and the Cannonball tree. It is distributed in the tropical regions of northern South America and Southern Caribbean. It has several medicinal properties. It is used to treat hypertension, tumours, pain, inflammatory processes, cold, stomach ache, skin diseases, malaria, wounds and toothache. Methods: The fruits of Couroupita guianensis were extracted with chloroform. Antimicrobial, antimycobacterial and antibiofilm forming activities of the chloroform extract were investigated. Quantitative estimation of Indirubin, one of the major constituent, was identified by HPLC. Results: Chloroform extract showed good antimicrobial and antibiofilm forming activities; however it showed low antimycobacterial activity. The zones of inhibition by chloroform extract ranged from 0 to 26 mm. Chloroform extract showed effective antibiofilm activity against Pseudomonas aeruginosa starting from 2 mg/mL BIC, with 52% inhibition of biofilm formation. When the chloroform extract was subjected to HPLC-DAD analysis, along with Indirubin standard, in the same chromatographic conditions, it was found that Indirubin was one of the major compounds in this plant (0.0918% dry weight basis). Conclusions: The chloroform extract showed good antimicrobial and antibiofilm properties. Chloroform extract can be evaluated further in drug development programmes.

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Inhibition of Vibrio biofilm formation by a marine actinomycete strain A66

Cited by 175 publications

References 33 publications

An Introduction to Actinobacteria

An Introduction to Actinobacteria

Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm

Impairment of the Bacterial Biofi lm Stability by Triclosan

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