Inhibition of verotoxin (VT) 2 absorption into systemic blood from intestine by repeated administration of bovine immune colostral antibody against VT2 in mice

Abstract

These results suggest that absorption of VT2 from the intestine was inhibited by repeated administration of bovine immune colostral antibody against VT2 at early stages of Escherichia coli O157:H7 infection, whereas VT2 in the intestine remained at low levels.

The Role of Escherichia coli Shiga Toxins in STEC Colonization of Cattle

“…application to calves of Shiga toxoids led to substantial anti-Stx1 as well as anti-Stx2 titres without causing any adverse effects [72]. Vaccination-induced anti-Stx bovine antibodies neutralize the biological activity of the holotoxin in vitro [72] in in vivo [140][141][142] in various models. The poor immunogenicity of Stxs upon natural exposure and the absence of Stx-specific seroconversion seen after experimental STEC infection of calves [29,138], cannot be explained by a damage of B cells by Stxs also.…”

“…Immunization of pregnant cows with Stxs results in colostrums enriched in anti-Stx1 and anti-Stx2 [142,223]. Such colostrums, or preparations thereof, reduce STEC shedding by experimentally infected dogs [140] and mitigate Stx2 absorption from the intestine of mice [141]. They protect weaned mice from a lethal dose of STEC O157:H7, avoid the inhibition of water absorption induced by E. coli O157:H7 in human colon fragments, and mitigate the pathogenicity of STEC O157:H7 in rat colon loops, although some of these effects were to be partially attributed to bLF in the preparations [142].…”