Inhibition of verocytotoxigenic Escherichia coli in model broth and rumen systems by carvacrol and thymol

Rivas, Lucía; McDonnell, Mary J.; Burgess, Catherine M.; O’Brien, Martin; Navarro-Villa, A.; Fanning, Séamus; Duffy, Geraldine

doi:10.1016/j.ijfoodmicro.2010.01.029

Cited by 101 publications

(47 citation statements)

References 47 publications

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“…Various methods have been used to prepare free EOs/EOCs, and thus, comparison of various studies is a challenge. In most studies, organic solvents, such as dimethyl sulfoxide (14), methanol (15,16), ethylene glycol (17), and ethanol (18)(19)(20), are used to prepare stock solutions that are diluted in a test medium to obtain the overall test concentration. The use of water-miscible organic solvents changes the polarity of the aqueous phase when the stock solution is blended with a liquid medium.…”

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confidence: 99%

Impacts of Sample Preparation Methods on Solubility and Antilisterial Characteristics of Essential Oil Components in Milk

Chen

Davidson

Zhong

2014

Appl Environ Microbiol

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Essential oil components (EOCs) have limited water solubility and are used at much higher concentrations in complex food matrices than in growth media to inhibit pathogens. However, the correlation between solubility and activity has not been studied. The objective of this work was to characterize the solubility of EOCs in solvents and milk and correlate solubility with antilisterial activity. The solubilities of four EOCs, thymol, carvacrol, eugenol, and trans-cinnamaldehyde, in water was significantly increased in the presence of 5% (vol/vol) ethanol. In milk, the solubility of EOCs was lower than in water, with lower solubility in higher-fat milk. EOCs applied to milk as stock solutions (in 95% aqueous ethanol) enabled quicker dissolution and higher solubility in milk serum than other methods of mixing, such as end to end, and greater reductions of Listeria monocytogenes Scott A after 0 and 24 h. When the EOC concentration detected in milk serum was above the minimum bactericidal concentration, complete inhibition of L. monocytogenes in tryptic soy broth resulted. Therefore, the antilisterial properties in milk could be correlated with the solubility by comparison to the minimum inhibitory or bactericidal concentrations of EOCs. While the EOCs applied using ethanol generally had solubility and activity characteristics superior to those of other mixing methods, ethanol is not used to a great extent in nonfermented foods. Therefore, mixing methods without an organic solvent may be more readily adaptable to enhancing the distribution of EOCs in complex food systems.

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confidence: 99%

Impacts of Sample Preparation Methods on Solubility and Antilisterial Characteristics of Essential Oil Components in Milk

Chen

Davidson

Zhong

2014

Appl Environ Microbiol

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“…The antimicrobial activity of thymol (1.36% in the essential oil of S. buxifolia) has been confirmed on bacteria such as E. coli (Rivas et al 2010). Thymol has been shown to cause disruption of the cellular membrane, inhibition of ATPase activity, and release of intracellular ATP and other constituents (Raybaudi-Massilia et al 2006, Viuda-Martos et al 2011).…”

Section: Resultsmentioning

confidence: 98%

Scutia buxifolia Reissek essential oil: in vitro antioxidant and antimicrobial activities

Boligon

Piana

Brum

et al. 2014

An. Acad. Bras. Ciênc.

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The volatile oil from the stem bark of Scutia buxifolia (Rhamnaceae) has been obtained by hydrodistillation and analyzed by GC-MS. Twenty-one components were identified representing 99.93 % of the total oil composition, spathulenol (35.87%), β-cubebene (17.26%), germacrene D (6.43%), linalool (5.19%), carvacrol (4.05%) were the main components of S. buxifolia essential oil. Antioxidant and antimicrobial properties of the essential oil were evaluated by free radical scavenging (DPPH) assay and micro broth dilution method, respectively. S. buxifolia essential oil presented interesting radical scavenging activity (IC 50 = 15.03 ± 0.11 μg/mL). The antibacterial assay showed that S. buxifolia stem bark essential oil was moderately active against the Staphylococcus aureus and Micrococcus sp. (MIC = 500 μg/mL) and Escherichia coli (250 μg/mL). To the best of our knowledge, this is the first study on the composition, antioxidant and antimicrobial activities of essential oil from the S. buxifolia collected from Brazil.

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“…The sensitivity of microorganisms to thyme oil has been reported by Girova et al (2010). More so, the susceptibility of thymol, which is the most important constituent of thyme oil, has been examined by Snoussi et al (2008), Rivas et al (2010), Schirmer and Langsrud (2010) and Szczepaniak et al (2011).…”

Section: Discussionmentioning

confidence: 99%

Antagonistic activity of Thymus vulgaris extracts against Vibrio species isolated from seafoods

Oramadike

Ogunbanwo

2017

J Food Sci Technol

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This study examined the antibacterial activity of Thymus vulgaris on multiple antibiotic resistant (MAR) Vibrio parahaemolyticus and Vibrio fluvialis isolated from shrimps. The ethanol extract of T. vulgaris antibacterial properties was assessed using the agar diffusion method. Survival of test organisms in shrimp meat using different concentrations of T. vulgaris was done using standard method. The quantitative and qualitative phytochemical tests of T. vulgaris extract were determined. The ethanol extract had antimicrobial activities on the test organisms showing 20.00 ± 0.0 and 23.00 ± 0.0 mm zone of inhibition on V. parahaemolyticus and V. fluvialis respectively. T. vulgaris completely decreased microbial load of V. parahaemolyticus and V. fluvialis in 150 and 60 min, respectively. The phytochemical screening for the ethanol extract reported phenol, alkaloids, tannin, saponin, anthraquinone flavonoid and cardiac glycoside as 51. 76, 26.60, 6.76, 54.33, 30.35 89.65 and 18.23 mg/100 g, respectively. This study reveals the antibacterial property of T. vulgaris on the MAR Vibrio species.

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Inhibition of verocytotoxigenic Escherichia coli in model broth and rumen systems by carvacrol and thymol

Cited by 101 publications

References 47 publications

Impacts of Sample Preparation Methods on Solubility and Antilisterial Characteristics of Essential Oil Components in Milk

Impacts of Sample Preparation Methods on Solubility and Antilisterial Characteristics of Essential Oil Components in Milk

Scutia buxifolia Reissek essential oil: in vitro antioxidant and antimicrobial activities

Antagonistic activity of Thymus vulgaris extracts against Vibrio species isolated from seafoods

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