Inhibition of UDP-&lt;i&gt;N&lt;/i&gt;-acetylglucosamine Transport by Diflubenzuron across Biomembranes of the Midgut Epithelial Cells in the Cabbage Armyworm, &lt;i&gt;Mamestra brassicae&lt;/i&gt; L.

喬, 満井; 満, 多田; 智恵子, 信沢; 勇, 山口

doi:10.1584/jpestics.10.55

Cited by 19 publications

(4 citation statements)

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“…Five genes encoding transporters with less‐documented roles in insecticide resistance are also worth noting: three UDP‐N‐acetylglucosamine (UDP‐GlcNAc) transporters and two major facilitator superfamily domain‐containing (MFS) proteins. Although the precise mechanism of chitin synthesis inhibition by benzoylureas is uncertain, experimental evidence suggests inhibition of N ‐acetylglucosamine incorporation into chitin is a downstream effect of benzoylurea action on sulfonylurea receptors (Matsumura, ; Mitsui et al., ; Sun et al., ). Novaluron (Rimon ® , Chemtura, Philadelphia, PA) tolerance has been observed among historically naïve L. decemlineata larvae (Cutler et al., ), suggesting a role of cross‐resistance with other chemistries, but modification of UDP‐GlcNAc transporters could represent a novel, benzoylurea‐specific adaptation.…”

Section: Discussionmentioning

confidence: 99%

Landscape genomics of Colorado potato beetle provides evidence of polygenic adaptation to insecticides

et al. 2017

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The ability of insect pests to rapidly and repeatedly adapt to insecticides has long challenged entomologists and evolutionary biologists. Since Crow's seminal paper on insecticide resistance in 1957, new data and insights continue to emerge that are relevant to the old questions about how insecticide resistance evolves: such as whether it is predominantly mono- or polygenic, and evolving from standing vs. de novo genetic variation. Many studies support the monogenic hypothesis, and current management recommendations assume single- or two-locus models. But inferences could be improved by integrating data from a broader sample of pest populations and genomes. Here, we generate evidence relevant to these questions by applying a landscape genomics framework to the study of insecticide resistance in a major agricultural pest, Colorado potato beetle, Leptinotarsa decemlineata (Say). Genome-environment association tests using genomic variation from 16 populations spanning gradients of landscape variables associated with insecticide exposure over time revealed 42 strong candidate insecticide resistance genes, with potentially overlapping roles in multiple resistance mechanisms. Measurements of resistance to a widely used insecticide, imidacloprid, among 47 L. decemlineata populations revealed heterogeneity at a small (2 km) scale and no spatial signature of origin or spread throughout the landscape. Analysis of nucleotide diversity suggested candidate resistance loci have undergone varying degrees of selective sweeps, often maintaining similar levels of nucleotide diversity to neutral loci. This study suggests that many genes are involved in insecticide resistance in L. decemlineata and that resistance likely evolves from both de novo and standing genetic variation.

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Section: Discussionmentioning

confidence: 99%

Landscape genomics of Colorado potato beetle provides evidence of polygenic adaptation to insecticides

et al. 2017

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“…Apparently, the insect polymerase obeys the same topography. However, studying cultured insect integuments, Mitsui and coworkers [63,64] claimed that the catalytic site faces the outer cell surface. Consequently, such a location invokes a transport system for the high molecular weight UDP-GlcNAc substrate which cannot diffuse through the plasma membrane.…”

Section: Chitin From Polymer To Microfibrilmentioning

confidence: 99%

“…The exact biochemical lesion inflicted by these inhibitors is not known. Among the suggestions forwarded were 1) enhanced activities of phenoloxidase and chitinase [66]; 2) inhibition of proteases which convert the inactive zymogenic chitin synthase into an active enzyme [67]; 3) inhibition of DNA synthesis [68]; 4) inhibition of influx of nucleosides [69]; 5) interference with the insect hormonal balance [70]; and 6) inhibition of the speculative GlcNAc transporter [64]. Some of the suggested mechanisms of action such as 1, 3, and 4 have been regarded as secondary effects [3].…”

Section: Chitin From Polymer To Microfibrilmentioning

confidence: 99%

Chitin synthesis and degradation as targets for pesticide action

Cohen

1993

Arch Insect Biochem Physiol

139

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Various pesticides are being used to destabilize, perturb, or inhibit crucial biochemical and physiological targets related to metabolism, growth, development, nervous communication, or behavior in pestiferous organisms. Chitin is an eukaryotic extracellular aminosugar biopolymer, massively produced by most fungal systems and by invertebrates, notably arthropods. Being an integral supportive component in fungal cell wall, insect cuticle, and nematode egg shell, chitin has been considered as a selective target for pesticide action. Throughout the elaborate processes of chitin formation and deposition, only the polymerization events associated with the cell membrane compartment are so far available for chemical interference. Currently, the actinomycetes-derived nucleoside peptide fungicides such as the polyoxins and the insecticidal benzoylaryl ureas have reached commercial pesticide status. The polyoxins and other structurally-related antibiotics like nikkomycins are strong competitive inhibitors of the polymerizing enzyme chitin synthase. The exact biochemical lesion inflicted by the benzoylaryl ureas is still elusive, but a post-polymerization event, such as translocation of chitin chains across the cell membrane, is suggested. Hydrolytic degradation of the chitin polymer is essential for hyphal growth, branching, and septum formation in fungal systems as well as for the normal molting of arthropods. Recently, insect chitinase activity was strongly and specifically suppressed by allosamidin, an actimomycetes-derived metabolite. In part, the defense mechanism in plants against invasion of pathogens is associated with induced chitinases. Chitin, chitosan, and their oligomers are able to act as elicitors which induce enhanced levels of chitinases in various plants. Lectins which bind to N-acetyl-D-glucosamine strongly interfere with fungal and insect chitin synthases. Plant lectins with similar properties may be involved in plant-pathogen interaction inter alia by suppressing fungal invasion.

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“…Benzoylphenylureas interfere with chitin deposition, probably by inhibition of the membrane transport of the precursor UDP-N-acetylglucosamine in chitin synthezising cells (Mitsui et al, 1985). In addition to their insecticidal spectrum of activity, the first generation of benzoylphenylureas, with diflubenzuron as the most widely investigated representative, has an acaricidal activity which is limited to Eriophyidae (Grosscurt, 1978).…”

Section: Introductionmentioning

confidence: 99%

Factors influencing the acaricidal activity of flucycloxuron

Grosscurt

1993

Entomologia Exp Applicata

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Contact activities of flucycloxuron on immature stages of the two‐spotted spider mite (Tetranychus urticae (Koch)) and the European red mite (Panonychus ulmi (Koch)) gradually decrease in the successive developmental stages. The levels of contact activity of flucycloxuron on larvae and protonymphs of T. urticae and P. ulmi are of the same order. Deutonymphs of T. urticae are less susceptible to contact activity than the similar stage of P. ulmi. In adult T. urticae, the transovarial ovicidal activity was used as an indicator for cuticular penetration. More than 90% of the maximal penetration into adult mites occurs within 8 h. Reversibility of the transovarial activity was not observed after 24 h, but did occur after a subsequent 48 h stay on untreated leaves. The ovo‐larvicidal activity of flucycloxuron on P. ulmi after treatment of apple leaves is strongly negatively influenced by leaf age, partly by lower retention of the spray liquid on the leaves. Leaf penetration was measured by application of flucycloxuron on leaf uppersides and assessment of the transovarial activity in mites (P. ulmi or T. urticae) infested on the undersides, one day after treatment. In this test system, leaf penetration was found to be strongly species dependent. Penetration was high in cucumber, moderate in French beans, cotton, roses and strawberry, but low in apple and pepper. Leaf penetration in French bean plants is drastically reduced at increasing leaf age. The overall positive effect of increase in relative air humidity on leaf penetration, is statistically highly significant (P = 0.001) for French beans and almost significant (P = 0.08) for cucumbers. With T. urticae on French bean it was found that in this test flucycloxuron needs more than one day for maximal leaf penetration. Although in apple leaves penetration from uppersides was low, penetration from undersides was much higher. The surfactants Arkopal N130, Silwet L‐77 and X2‐5309 enhance penetration from leaf undersides.

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Inhibition of UDP-<i>N</i>-acetylglucosamine Transport by Diflubenzuron across Biomembranes of the Midgut Epithelial Cells in the Cabbage Armyworm, <i>Mamestra brassicae</i> L.

Cited by 19 publications

References 2 publications

Landscape genomics of Colorado potato beetle provides evidence of polygenic adaptation to insecticides

Landscape genomics of Colorado potato beetle provides evidence of polygenic adaptation to insecticides

Chitin synthesis and degradation as targets for pesticide action

Factors influencing the acaricidal activity of flucycloxuron

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