Inhibition of tyrosinase by green tea components

No, Jae Kyung; Soung, Do Y.; Kim, You Jung; Shim, Kyung Hee; Jun, Yeong Soo; Rhee, Sook‐Hee; Yokozawa, Takako; Chung, Hae Young

doi:10.1016/s0024-3205(99)00492-0

Cited by 186 publications

(122 citation statements)

References 12 publications

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“…and polyphenols like kaempferol [9], quercetin [10], etc. The polyphenol group is widely distributed in natural plants from the bark, root to the leaves and peels, which provides a wide choice for the source of tyrosinase inhibitors such as green tea [11], black rice bran [12] and grape seed [13].…”

Section: Introductionmentioning

confidence: 99%

Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts

Zhang

Lü

Lin

et al. 2007

Journal of Enzyme Inhibition and Medicinal Chemistry

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The inhibitory effects of nobiletin and hesperidin from citrus peel crude extracts on tyrosinase diphenolase activity are evaluated. IC 50 of nobiletin and hesperidin is 1.49 mM and 16.08 mM, respectively and their inhibition mechanism is competitive type with K i ¼ 2.82 mM and noncompetitive with K i ¼ 9.16 mM, respectively. Crude extracts from citrus peel (C. unshiu Marc.) were extracted with 95% ethanol and fractionated by petroleum ether (PCPE). The ethanol phase (ECPE) was further desorbed from macroporous adsorption resin (FGRE). Their IC 50 values were 8.09 mg/mL, 7.53 mg/mL and 4.80 mg/mL, respectively. Their inhibition on melanogenesis in B16 mouse melanoma cells was also evaluated. FGRE showed a significant inhibition (42.5% at 31.25 mg/mL, p , 0.01) while hesperidin showed almost no inhibition. Nobiletin and PCPE give efficacious antiproliferation effects on B16 mouse melanoma cell with IC 50 values 88.6 mM and 62.96 mg/mL, respectively, by the MTT test. Hesperidin and other crude extracts showed very low cytotoxity to the B16 cell.

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Section: Introductionmentioning

confidence: 99%

Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts

Zhang

Lü

Lin

et al. 2007

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…lowed the method of Gregory and Bendall (1966) and Kyung No et al (1999) with modifications. All samples used for this study were extracted with methanol and filtered.…”

Section: Methodsmentioning

confidence: 99%

Selecting plants with increased total polyphenol oxidases in the genus Trifolium

Jakešová¹,

Řepková²,

Nedĕlník³

et al. 2015

CZECH J GENET PLANT

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Jakešová H., Řepková J., Nedělník J., Hampel D., Dluhošová J., Soldánová M., Ošťádalová M. (2015): Selecting plants with increased total polyphenol oxidases in the genus Trifolium. Czech J. Genet. Plant Breed., 51: 155-161.One of the aims in red clover (Trifolium pratense) breeding is to increase the polyphenol oxidase (PPO) activity, which may effectively reduce protein breakdown in silage and when cattle are fed by fresh clover. We analysed total PPO activity spectrophotometrically and on the level of gene expression using real-time quantitative PCR in single plants derived from an interspecific T. pratense × T. medium hybrid. Experiments were performed for two years and evaluated according to the general linear model with three factors (family, year, and cut). The analysis revealed considerable variability in total PPO activity between individuals and between families. Four families and two individuals with significantly higher PPO activity were selected. Their PPO activity ranged from 3.411 to 3.547 mkatal/min/g and from 4.041 to 5.731 mkatal/min/g, respectively, in comparison with the control variety Amos (2.370 mkatal/min/g). The majority of PPO transcripts were expressed by the two genes PPO1/5 and PPO2. In some genotypes, the PPO5 gene was expressed. Quantitative PCR confirmed the highest activity of PPO genes in seven hybrid plants with higher DNA contents corresponding to 30 chromosomes with 815 013 copies per plant. Our results indicate the suitability of combining two methods for improved selection: initial expression analysis to assess the PPO transcript level indicating gene activity and subsequent enzymatic assay.

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“…K i value of nobiletin is estimated as 2.82 mM (the inset of Figure 7, and Table I). This suggests that this polymethoxylated flavone, nobiletin, effectively binds the active site reversibly (chelating copper in the enzyme), and its active radical is an exception to the previous understanding of tyrosinase inhibition of flavonoids in that it is not a free 3-hydroxy group [4], not a-keto group [44] and not the flavon-3-ol skeleton with a galloyl moiety at the 3-position [11], but a methoxyl group on the flavone skeleton.…”

Section: Inhibition Of Diphenolase Activity Of Tyrosinase By Citrus Ementioning

confidence: 95%

“…; polyphenols like kaempferol [9], quercetin [10], etc. The polyphenol group is widely distributed in natural plants from the bark and root to leaves and peels, which provides a wide choice for the source of tyrosinase inhibitors such as green tea [11], black rice bran [12] and grape seed [13].…”

Section: Introductionmentioning

confidence: 99%

Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts

Zhang

Lü

Lin

et al. 2007

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

The inhibitory effects of nobiletin and hesperidin from citrus peel crude extracts on tyrosinase diphenolase activity have been evaluated. IC 50 of nobiletin and hesperidin were 1.49 mM and 16.08 mM, respectively and their inhibition mechanisms are competitive type with inhibition constant (K i ) 2.82 mM and noncompetitive type with K i 9.16 mM, respectively. Crude extracts from citrus peel (C. unshiu Marc.) were extracted with 95% ethanol and fractionated by petroleum ether (PCPE). The ethanol phase (ECPE) was further desorbed from macroporous adsorption resin (FGRE). Their IC 50 values were 8.09 mg/mL, 7.53 mg/mL and 4.80 mg/mL, respectively. Their inhibition of melanogenesis in B16 mouse melanoma cells was also evaluated. FGRE showed a significant inhibition (42.48% at 31.25 mg/mL, p , 0.01) while hesperidin showed almost no inhibition. Nobiletin and PCPE gave efficacious antiproliferation effects on the B16 mouse melanoma cell with IC 50 values 88.6 mM and 62.96 mg/mL, respectively, through the MTT test. Hesperidin and other crude extracts showed very low cytotoxity to the B16 cell.

show abstract

Inhibition of tyrosinase by green tea components

Cited by 186 publications

References 12 publications

Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts

Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts

Selecting plants with increased total polyphenol oxidases in the genus Trifolium

Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts

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