Inhibition of TNF-α, and NF-κB and JNK pathways accounts for the prophylactic action of the natural phenolic, allylpyrocatechol against indomethacin gastropathy

Yadav, Sudhir Kumar; Adhikary, Biplab; Bandyopadhyay, Sandip K.; Chattopadhyay, Subrata

doi:10.1016/j.bbagen.2013.03.013

Cited by 30 publications

(30 citation statements)

References 48 publications

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“…This anti-inflammatory effect of sEHI was expected on the basis of previous observations (Schmelzer et al, 2005;Zhang et al, 2012;Kodani and Hammock, 2015). OME was also reported to decrease the levels of MPO and TNF-a in indomethacin-induced ulcers (Chatterjee et al, 2012;Yadav et al, 2013). In our study, pretreatment with OME decreased the level of MPO and TNF-a significantly, consistent with OME having significant anti-inflammatory activity in addition to inhibiting proton pumps (Fig.…”

Section: Resultssupporting

confidence: 78%

Anti-Ulcer Efficacy of Soluble Epoxide Hydrolase Inhibitor TPPU on Diclofenac-Induced Intestinal Ulcers

Goswami

Wan

Yang

et al. 2016

Journal of Pharmacology and Experimental Therapeutics

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Proton pump inhibitors such as omeprazole (OME) reduce the severity of gastrointestinal (GI) ulcers induced by nonsteroidal anti-inflammatory drugs (NSAIDs) but can also increase the chance of dysbiosis. The aim of this study was to test the hypothesis that preventive use of a soluble epoxide hydrolase inhibitor (sEHI) such as TPPU can decrease NSAID-induced ulcers by increasing anti-inflammatory epoxyeicosatrienoic acids (EETs). Dose-[10, 30, and 100 mg/kg, by mouth (PO)] and time-dependent (6 and 18 hours) ulcerative effects of diclofenac sodium (DCF, an NSAID) were studied in the small intestine of Swiss Webster mice. Dose-dependent effects of TPPU (0.001-0.1 mg/kg per day for 7 days, in drinking water) were evaluated in DCF-induced intestinal toxicity and compared with OME (20 mg/kg, PO). In addition, the effect of treatment was studied on levels of Hb in blood, EETs in plasma, inflammatory markers such as myeloperoxidase (MPO) in intestinal tissue homogenates, and tissue necrosis factor-a (TNF-a) in serum. DCF dose dependently induced ulcers that were associated with both a significant (P , 0.05) loss of Hb and an increase in the level of MPO and TNF-a, with severity of ulceration highest at 18 hours. Pretreatment with TPPU dose dependently prevented ulcer formation by DCF, increased the levels of epoxy fatty acids, including EETs, and TPPU's efficacy was comparable to OME. TPPU significantly (P , 0.05) reversed the effect of DCF on the level of Hb, MPO, and TNF-a. Thus sEHI might be useful in the management of NSAID-induced ulcers.

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Section: Resultssupporting

confidence: 78%

Anti-Ulcer Efficacy of Soluble Epoxide Hydrolase Inhibitor TPPU on Diclofenac-Induced Intestinal Ulcers

Goswami

Wan

Yang

et al. 2016

Journal of Pharmacology and Experimental Therapeutics

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“…O mecanismo de indução de úlcera é mediado por espécies reativas de oxigênio (ROS) (Oyagi et al, 2010;Yadav et al, 2013). No extrato etanólico de P. alnifolia foi identificado a presença de rutina (Andrade et al, 2012), flavonoide com elevada capacidade antioxidante (Yang et al, 2008).…”

Section: Resultado E Discussãounclassified

Atividade gastroprotetora do extrato etanólico de Pavonia alnifolia A.St.-Hil.

Ewald

Loyolla

Pereira

et al. 2015

Rev. bras. plantas med.

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RESUMO:No Brasil, a família Malvaceae está representada por aproximadamente 200 espécies e algumas foram descritas como gastroproteroras. Pavonia alnifolia A.St.-Hil. (Malvaceae) foi selecionada após uma abordagem quimiossistemática, considerando-se sua potencial capacidade em prevenir lesões gástricas. Assim, a atividade gastroprotetora do extrato etanólico de caules de P. alnifolia foi avaliada utilizando o modelo de indução aguda da lesão gástrica por etanol acidificado em camundongos. Além disso, foram quantificados o teor de flavonóides, pelo método de cloreto de alumínio, e de polifenóis, pelo método Folin-Ciocalteu, uma vez que a relação desses componentes com a proteção gástrica foi evidenciada. Os ensaios apontaram redução acentuada das lesões gástricas em camundongos tratados com o extrato da planta em todas as doses ensaiadas (10, 100 e 300 mg/kg). Esse efeito pode estar relacionado com a presença de polifenóis, cujo teor encontrado foi 74,3 ± 7,5 µg equivalente de pirogalol/mg do material vegetal examinado e 82,7 ± 7,1 µg equivalente de pirogalol /mg da amostra no extrato preparado por percolação e teor de flavonoides totais, que por sua vez apresentou um resultado de 17,1 ± 1,4 µg/mg de extrato. O extrato apresentou proteção da mucosa gástrica e este efeito pode estar relacionado à presença dos polifenóis e flavonóides encontrados. Palavras-chave:Pavonia, Malvaceae, gastroproteção, flavonóides, polifenóis.ABSTRACT: Gastro protective activity of the Pavonia alnifolia A.St.-Hil. extract. In Brazil, the Malvaceae family is represented by at about 200 species. Some of those species are known as gastro protective ones. The Pavonia alnifolia A.St.-Hil (Malvaceae) was selected after a chemosystematic approach. The gastro preventive activity of the ethanol extract of stems Pavonia alnifolia was evaluated through the use of the Ethanol:chlroridric acid model on mice. The quantification of the total flavonoids (aluminum chloride method) and total polyphenols (Folin-Ciocalteu method) was also performed since the relation of those components with gastric protection has been previously highlighted. The tests showed a significant reduction of the ulcer formation in the mice treated with the plant extract (10, 100 and 300 mg/kg). This effect may be related to the presence of polyphenols whose content was found to be 74.3 ± 7.5 µg/mg of vegetal material and 82.7 ± 7.1 µg/mg of crude extract and flavonoids, which in turn showed a content of 17.1 ± 1.4 µg/mg dry extract.

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“…)-induced acute stomach ulceration in mice. Moreover, the protocols of IND-mediated ulceration in mice, and its healing by several anti-ulcer agents have also been extensively studied by our group [23,24]. Hence the present study was carried out under the same experimental conditions.…”

Section: Discussionmentioning

confidence: 96%

“…Group I mice served as normal control, while ulceration in the groups II-V mice was induced by IND (18 mg kg − 1 , p. o., single dose) dissolved in distilled water and suspended in the vehicle, gum acacia (2%). The dose of IND and the treatment regime (drug doses and period of treatment) were standardized in our earlier studies [9,23,24]. The mice of groups I and III (referred to as control) were given the daily oral dose of vehicle (gum acacia in distilled water, 0.2 ml).…”

Section: Experimental Protocol For Ulceration and Biochemical Studiesmentioning

confidence: 99%

dl-trans-3,4-Dihydroxy-1-selenolane (DHSred) heals indomethacin-mediated gastric ulcer in mice by modulating arginine metabolism

Chakraborty

Yadav

Subramanian

et al. 2014

Biochimica et Biophysica Acta (BBA) - General Subjects

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Inhibition of TNF-α, and NF-κB and JNK pathways accounts for the prophylactic action of the natural phenolic, allylpyrocatechol against indomethacin gastropathy

Cited by 30 publications

References 48 publications

Anti-Ulcer Efficacy of Soluble Epoxide Hydrolase Inhibitor TPPU on Diclofenac-Induced Intestinal Ulcers

Anti-Ulcer Efficacy of Soluble Epoxide Hydrolase Inhibitor TPPU on Diclofenac-Induced Intestinal Ulcers

Atividade gastroprotetora do extrato etanólico de Pavonia alnifolia A.St.-Hil.

dl-trans-3,4-Dihydroxy-1-selenolane (DHSred) heals indomethacin-mediated gastric ulcer in mice by modulating arginine metabolism

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