Inhibition of TNF reduces mechanical orofacial hyperalgesia induced by Complete Freund’s Adjuvant by a TRPV1-dependent mechanism in mice

Lis, Krzysztof; Grygorowicz, Tomasz; Cudna, Agnieszka; Szymkowski, David E.; Bałkowiec-Iskra, Ewa

doi:10.1016/j.pharep.2017.05.013

Cited by 12 publications

(7 citation statements)

References 48 publications

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“…These results suggested that these assessment methods, including the von Frey filament test, the bite force test, and eating pattern analysis, could be used to analyse different TMD mouse models. To verify the reliability of these methods, other types of TMD models are also needed, including mechanical stimulus-induced models, in which specific appliances have been employed to develop abnormal occlusal conditions, 39 , 40 the noxious stimulus model by orofacial formalin injection, 41 the TMJ inflammation mouse model, which is induced by the injection of complete Freund’s adjuvant, 42 , 43 and genetic mouse models. 13 , 44 Moreover, these methods should be further optimised in future experiments.…”

Section: Discussionmentioning

confidence: 99%

Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders

et al. 2020

Int J Oral Sci

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Orofacial pain or tenderness is a primary symptom associated with temporomandibular joint (TMJ) disorders (TMDs). To understand the pathological mechanisms underlying TMDs, several mouse models have been developed, including mechanical stimulus-induced TMD and genetic mouse models. However, a lack of feasible approaches for assessing TMD-related nociceptive behaviours in the orofacial region of mice has hindered the in-depth study of TMD-associated mechanisms. This study aimed to explore modifications of three existing methods to analyse nociceptive behaviours using two TMD mouse models: (1) mechanical allodynia was tested using von Frey filaments in the mouse TMJ region by placing mice in specially designed chambers; (2) bite force was measured using the Economical Load and Force (ELF) system; and (3) spontaneous feeding behaviour tests, including eating duration and frequency, were analysed using the Laboratory Animal Behaviour Observation Registration and Analysis System (LABORAS). We successfully assessed changes in nociceptive behaviours in two TMD mouse models, a unilateral anterior crossbite (UAC)-induced TMD mouse model and a β-catenin conditional activation mouse model. We found that the UAC model and β-catenin conditional activation mouse model were significantly associated with signs of increased mechanical allodynia, lower bite force, and decreased spontaneous feeding behaviour, indicating manifestations of TMD. These behavioural changes were consistent with the cartilage degradation phenotype observed in these mouse models. Our studies have shown reliable methods to analyse nociceptive behaviours in mice and may indicate that these methods are valid to assess signs of TMD in mice.

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Section: Discussionmentioning

confidence: 99%

Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders

et al. 2020

Int J Oral Sci

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“…Genetic data indicate that TNFR1 plays an essential role for pain development in males (Dellarole et al, 2014). Accordingly, it was shown that intraperitoneal administration of XPro1595 prevented complete Freund's adjuvant (CFA)induced mechanical hypersensitivity in male mice in a model of local CFA-induced model of orofacial pain (Lis et al, 2017). Similar, after CCI, systemic application of XPro1595 alleviated mechanical allodynia in males.…”

Section: Selective Neutralization Of Stnf By Dominant-negative Tnf Mumentioning

confidence: 99%

Selective Targeting of TNF Receptors as a Novel Therapeutic Approach

Fischer

Kontermann

Pfizenmaier

2020

Front. Cell Dev. Biol.

152

146

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Tumor necrosis factor (TNF) is a central regulator of immunity. Due to its dominant proinflammatory effects, drugs that neutralize TNF were developed and are clinically used to treat inflammatory and autoimmune diseases, such as rheumatoid arthritis, inflammatory bowel disease and psoriasis. However, despite their clinical success the use of anti-TNF drugs is limited, in part due to unwanted, severe side effects and in some diseases its use even is contraindicative. With gaining knowledge about the signaling mechanisms of TNF and the differential role of the two TNF receptors (TNFR), alternative therapeutic concepts based on receptor selective intervention have led to the development of novel protein therapeutics targeting TNFR1 with antagonists and TNFR2 with agonists. These antibodies and bio-engineered ligands are currently in preclinical and early clinical stages of development. Preclinical data obtained in different disease models show that selective targeting of TNFRs has therapeutic potential and may be superior to global TNF blockade in several disease indications.

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“…Current research suggests that inflammation is an influential participant factor in the pathophysiology of cerebral ischemia reperfusion injury. On one hand, inflammatory factors such as TNF-α and IL-1β activated by the microglia in the ischemic zone pose a toxic effect on ischemia brain neurons, thus exacerbate the damage of neurons (Lis et al, 2017). On the other hand, I/R can lead to the disrupt open of blood brain barrier (BBB), and peripheral infiltration of inflammatory cells into ischemic brain tissue, thus aggravate inflammatory lesions (Shichita et al, 2012).…”

Section: Western Blot Test Resultsmentioning

confidence: 99%

Study on the mechanism and therapeutic effect of Antelope horn treatment on cerebral ischemia reperfusion injury of mouse

Wang

Jin

2021

Food Sci. Technol

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Materials and methods 2.1 Materials Cells and animals 50 C57BL/6 mice (20-15 g), male, 10 weeks old, body weight 23-25 g, purchased from the Experimental Animal Center in China. Reagents Antelope horn (Chinese Inspection of Biological Products and Drugs) DMEM medium (US BD Biosciences); anti-TNF-α, IL-1β, IFN-β rabbit polyclonal antibodies (Cell Signaling company); rabbit polyclonal anti-β-actin antibody (Santa Cruz Biotechnology, Santa company); fetal bovine serum (US BD Biosciences); mouse anti-human Bax antibody (Abcam Inc.); mouse anti-human Bcl-2 antibody (US BD Biosciences); rabbit anti-human Hsp 27, Hsp 27 antibodies (U.S. BD Biosciences).

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Inhibition of TNF reduces mechanical orofacial hyperalgesia induced by Complete Freund’s Adjuvant by a TRPV1-dependent mechanism in mice

Cited by 12 publications

References 48 publications

Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders

Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders

Selective Targeting of TNF Receptors as a Novel Therapeutic Approach

Study on the mechanism and therapeutic effect of Antelope horn treatment on cerebral ischemia reperfusion injury of mouse

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