Inhibition of the Proteinase and Esterase Activities of Trypsin and Chymotrypsin by Diisopropyl Fluorophosphate: Crystallization of Inhibited Chymotrypsin

Jansen, Eugene F.; Nutting, M.-D. Fellows; Jang, Rosie; Balls, A. K.

doi:10.1016/s0021-9258(18)56827-7

Cited by 232 publications

(10 citation statements)

References 11 publications

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“…2. Closely related proteins, chymotrypsinogen (Kunitz and Northrop, 1935) and diisopropyl-phosphoryl-ca-chymotrypsin (Jansen, Nutting, Jang, and Balls, 1949), which differs in one group from the active enzyme, are inactive.…”

Section: The Effect Of Varying the Phage Concentrationmentioning

confidence: 99%

The Effect of Proteolytic Enzymes on E. coli Phages and on Native Proteins

Northrop

1964

The Journal of General Physiology

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Lambda coli phage is not inactivated by chymotrypsin, trypsin, or ficin. T 2 phage is slowly inactivated by high concentrations of a-, -, y-, or A-chymotrypsin, but not by trypsin or ficin. PI phage is slowly inactivated by a-, -, or y-chymotrypsin, or ficin, more rapidly by A-chymotrypsin, and much more rapidly by trypsin. Crystalline egg albumin, crystalline serum albumin, E. coli nucleoprotein, and yeast nucleoprotein are hydrolyzed slowly by a-chymotrypsin. Yeast nucleoprotein, like P phage, is hydrolyzed more rapidly by A-chymotrypsin than by a-chymotrypsin, but not by trypsin or ficin. Neither phages nor native proteins were attacked by papain, carboxypeptidase, deoxyribonuclease, or ribonuclease.

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Section: The Effect Of Varying the Phage Concentrationmentioning

confidence: 99%

The Effect of Proteolytic Enzymes on E. coli Phages and on Native Proteins

Northrop

1964

The Journal of General Physiology

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“…It would be unlikely, therefore, that a free amine group (ammonium ion at pH 7 6) would be found in the region of the active site. It is of interest to note that acetylation of trypsin results in no loss of esterase activity (Jansen, Nutting, Jang & Balls, 1949). SUMMARY 1.…”

Section: -0 -mentioning

confidence: 99%

The amide groups of insulin

Sanger

Thompson

Kitai

1955

Biochemical Journal

187

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“…tt-Chymotrypsin-diisopropyl Phosphate Crystals.-Unusually large and well-formed crystals of the diisopropyl phosphate derivative DP of chymotrypsin were obtained from Mr. Eugene F. Jansen of the Western Regional Research Laboratory, who has described their preparation. 10 Hemoglobin Crystals.-Large bipyramidal crystals of human oxyhemoglobin were obtained from Dr. David L.…”

Section: Methodsmentioning

confidence: 99%