2019
DOI: 10.1007/s10753-019-01035-9
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Inhibition of the PI3K/AKT Signaling Pathway or Overexpression of Beclin1 Blocks Reinfection of Streptococcus pneumoniae After Infection of Influenza A Virus in Severe Community-Acquired Pneumonia

Abstract: Streptococcus pneumoniae (S. pneumoniae) and viruses are considered as primary risks of community-acquired pneumonia (CAP), and the effects of co-infection bacterial and virus in the prognosis of patients with severe CAP (SCAP) are poorly described. Therefore, this study is conducted to investigate the regulation of Beclin1-PI3K/AKT axis in reinfection of S. pneumoniae after influenza A virus in mice model of bronchoalveolar lavage fluid (BALF). Samples of sputum and BALF were collected from patients with SCAP… Show more

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Cited by 19 publications
(27 citation statements)
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“…Streptococcus pneumonia induces autophagy in A549 cells via a PI3K/AKT/mTOR-dependent mechanism ( 26 ). Yang et al ( 27 ) demonstrated that inhibition of PI3K/AKT/mTOR decreases the reinfection of Streptococcus pneumonia following influenza A virus infection in community-acquired pneumonia. Therefore, the present study aimed to explore whether the effects of miR-4485 on H1N1-induced severe pneumonia could be mediated via the PI3K/AKT/mTOR signaling pathway.…”
Section: Discussionmentioning
confidence: 99%
“…Streptococcus pneumonia induces autophagy in A549 cells via a PI3K/AKT/mTOR-dependent mechanism ( 26 ). Yang et al ( 27 ) demonstrated that inhibition of PI3K/AKT/mTOR decreases the reinfection of Streptococcus pneumonia following influenza A virus infection in community-acquired pneumonia. Therefore, the present study aimed to explore whether the effects of miR-4485 on H1N1-induced severe pneumonia could be mediated via the PI3K/AKT/mTOR signaling pathway.…”
Section: Discussionmentioning
confidence: 99%
“…After the 14-day treatment, mice were anesthetized with 3% pentobarbital sodium (P3761; Sigma-Aldrich, St Louis, MO, USA), and the broncho-alveolar lavage fluid (BALF) of the mice in each group was obtained by intratracheal lavage, as previously described [22]. The collected BALF (0.8 mL) was then centrifuged at 4°C for 10 min at 260×g, and the precipitate re-suspended in 0.5 mL sterile PBS.…”
Section: Measure Of Bacterial Loadmentioning
confidence: 99%
“…19 This method is extensively used to detect biological material; however, the technical limitations of ELISA include the requirement of a liquid system and long reaction time, which thereby limits the scope for method enhancement. [20][21][22] In this study, a magnetic microuidic ELISA system (MMF-ELISA) was designed to allow for rapid ELISA systems (Fig. 1).…”
Section: Introductionmentioning
confidence: 99%