Inhibition of the nuclear localization of [3H]estradiol in rat uterine tissue in vitro

Campbell, Paul S.; Albright, Carolyn; Wilson, H. J.; Bridges, R.

doi:10.1016/0022-4731(89)90512-8

Cited by 5 publications

(1 citation statement)

References 30 publications

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“…This may be related to additional, poorly understood functional roles of the different domains. Hormone status-dependent specific binding of steroid receptors to elements of the cytoskeleton (Campbell et al 1989;Akner et al 1990;Barsony and McKoy t992;Sierralta et al 1995), to stress proteins (Carson-Jurica et al 1990), to ribonucleoprotein particles (Sherman and Stevens 1984) or to cell membranes (Little et al 1972;Jungblut et al 1983;Szego and Pietras 1981;Pappas et al 1995), for example, may be responsible for the variations in epitope accessibility detected. The unmasking of uterine estradiol receptors with the microwave treatment alone apparently did not suffice for the complete retrieval of all their determinants.…”

Section: Discussionmentioning

confidence: 96%

Retrieval of estradiol receptor in paraffin sections of resting porcine uteri by microwave treatment. Immunostaining patterns obtained with different primary antibodies

Sierralta

Thole

1996

Histochem Cell Biol

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The unmasking of estradiol receptor in paraffin sections of Bouin's-fixed uterine tissue from ovariectomized gilts was attained with microwave treatment. Immunocytochemistry of the receptor was performed using a polyclonal or five monoclonal antibodies, two of which are commercially available, reacting with different domains of the protein and an amplified-peroxidase system for detection. With five of the antibodies, a predominance of nuclear staining was observed in cells of endometrial glands, while one monoclonal antibody (13H2), reacting with the receptor's domain E, showed a preference for the cytoplasmic receptor. In stroma, all antibodies detected more receptor in nuclei than in cytoplasm. In epithelium, the commercially available antibody H222, our monoclonals 13H2 and HT65, and the polyclonal antibody 402 demonstrated more receptor in cytoplasmic than in nuclear areas. In myometrium, the nuclei from longitudinal and ring muscles were definitely stained with the antibodies. We conclude that the accessibilities of the antibody epitopes of the receptor differ according to the functional uterine cell type.

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Section: Discussionmentioning

confidence: 96%

Retrieval of estradiol receptor in paraffin sections of resting porcine uteri by microwave treatment. Immunostaining patterns obtained with different primary antibodies

Sierralta

Thole

1996

Histochem Cell Biol

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Immunogold labelling of the cytoplasmic estradiol receptor in resting porcine endometrium

Sierralta

Thole²

1992

Cell Tissue Res.

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Serial sections of resting porcine endometrium were analyzed with the monoclonal antibody 13H2 using goat antimouse IgG/5 nm gold as secondary reagent or with either polyclonal antibodies from goat #402 or the rat monoclonal antibody H222, both in combination with protein G/12 nm gold. A modestly higher labelling of nuclei than of cytoplasm was seen only with the monoclonal antibody H222. Polyclonal #402 and monoclonal 13H2 showed fewer attachments over nuclear than over cytoplasmic areas. The highest densities of attachment and of predominantly cytoplasmic labelling were obtained with the monoclonal antibody 13H2. The results confirm the earlier assumption of a restricted accessibility of estradiol receptor in the cytoplasm of resting cells for immunoreagents.

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Nuclear localization of estrogen receptor-immunoreactivity in the preoptic area of female rats and its reduction by intraventricular colchicine treatment

Yuri

Kawata

1992

Neuroscience Letters

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Inhibition of the nuclear localization of [3H]estradiol in rat uterine tissue in vitro

Cited by 5 publications

References 30 publications

Retrieval of estradiol receptor in paraffin sections of resting porcine uteri by microwave treatment. Immunostaining patterns obtained with different primary antibodies

Retrieval of estradiol receptor in paraffin sections of resting porcine uteri by microwave treatment. Immunostaining patterns obtained with different primary antibodies

Immunogold labelling of the cytoplasmic estradiol receptor in resting porcine endometrium

Nuclear localization of estrogen receptor-immunoreactivity in the preoptic area of female rats and its reduction by intraventricular colchicine treatment

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