Inhibition of the mutagenicity of amino acid pyrolysis products by hemin and other biological pyrrole pigments

“…The unit of b-galactosidase activity was calculated according to the method of Miller. 23) Preparation of Activated Trp-P-1 Preparation of activated Trp-P-1 was carried out according to the method of Arimoto et al 24) UV Irradiation The cultures of the tester bacterial strain (S. typhimurium TA1535/pSK1002) were collected by centrifugation and suspended in 5 ml of 0.1 M phosphate buŠer. The cell suspensions were then poured into Petri dishes and exposed to UV light (4.0 J/m 2 ) for 20 s using a germicidical lamp at room temperature.…”

Section: Synthesis Of Disodium Isostearyl 2-o-l-ascorbyl Phosphate (Vmentioning

confidence: 99%

Antimutagenic Activity of a Novel Ascorbic Derivative, Disodium Isostearyl 2-O-L-Ascorbyl Phosphate

Hisama¹,

Matsuda²,

Shibayama³

et al. 2008

YAKUGAKU ZASSHI

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A novel amphiphilic vitamin C derivative, disodium isostearyl 2-O-L-ascorbyl phosphate (VCP-IS-2Na) possessing an alkyl chain of C 18 to a stable ascorbate derivative sodium L-ascorbic acid 2-phosphate (VCP-Na), was synthesized and evaluated as an anti-mutagen with suppressive eŠect on SOS-inducing activity on mutagen in the Salmonella typhimurium TA1535/pSK1002 umu test. VCP-IS-2Na was assayed with chemical mutagens, N-methyl-N′ -nitro-Nnitrosoguanidine (MNNG), 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (furylfuramide) and 4-nitroquinolin 1-oxide (4NQO), which do not require liver metabolizing enzymes. VCP-IS-2Na at a concentration of 0.40 mmol/ml suppressed 66.2％, 54.7％ and 60.2％ of the SOS-inducing activity on MNNG, furylfuramide, and 4NQO, and the 50％ inhibitory dose value (ID 50 ) was 0.12 mmol/ml, 0.26 mmol/ml, and 0.17 mmol/ml, respectively. In addition, VCP-IS-2Na was assayed with 2-aminoanthracene (2AA) and 3-amino-1,4-dimethyl-5H-pyrido [4,3-b]indole (Trp-P-1), which require liver metabolizing enzymes. To study the structure-activity relationship, L-ascorbic acid (VC) and VCP-Na were also assayed with all mutagens. VCP-IS-2Na, suppressed the chemical and physical mutagens-induced SOS response greater than VC and VCP-Na in the umu test. Also, the antimutagenic activities of VCP-IS-2Na, VC, and VCP-Na against MNNG and Trp-P-1 were assayed by the Ames test using the S. typhimurium TA100 strain. In summary, this research suggests that VCP-IS-2Na showed potent antimutagenic eŠects against chemical mutagens and UV irradiation.

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“…Chlorophyll has been demonstrated to suppress the mutagenicity of many toxicants [24][25][26][27]. The inhibitory action of chlorophyllins, the sodium and copper salts of chlorophyll, encompasses a wide variety of chemical compounds, including nitropyrenes (airborne and diesel emission particulates) and PAHs [26].…”

Section: Induction Of Mutation In Drosophila Melanogastermentioning

confidence: 99%

Induction of Mutation in Drosophila melanogaster fed a hexane extract of vegetables grown in soil contaminated with particulates from diesel engine exhaust

1999

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Inhibition of the mutagenicity of amino acid pyrolysis products by hemin and other biological pyrrole pigments

Cited by 119 publications

References 13 publications

Suppression of Mutagens-Induced SOS Response by Phytoncide Solution Using <i>Salmonella typhimurium</i> TA1535/pSK1002 <i>umu</i> Test

Suppression of Mutagens-Induced SOS Response by Phytoncide Solution Using <i>Salmonella typhimurium</i> TA1535/pSK1002 <i>umu</i> Test

Antimutagenic Activity of a Novel Ascorbic Derivative, Disodium Isostearyl 2-<i>O</i>-L-Ascorbyl Phosphate

Induction of Mutation in Drosophila melanogaster fed a hexane extract of vegetables grown in soil contaminated with particulates from diesel engine exhaust

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