Inhibition of the metabolism and motility of human spermatozoa by various alkaloids

Trifunac, Natalie P.; Bernstein, Gail A.

doi:10.1016/0010-7824(82)90020-8

Cited by 9 publications

(5 citation statements)

References 14 publications

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“…The treatment with chloroquine, which inhibits autophagy, decreased viability, motility, ATP content, and calcium concentration. In agreement with our results, it has previously been demonstrated that chloroquine can inhibit motility and viability in human spermatozoa, although in these previous reports the effects of chloroquine never have been linked with autophagy 63 64 . Hargreaves C.A.…”

Section: Discussionsupporting

confidence: 93%

Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility

Aparicio

Espino

Bejarano

et al. 2016

Sci Rep

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Macroautophagy (hereafter autophagy) is an evolutionarily highly conserved cellular process that participates in the maintenance of intracellular homeostasis through the degradation of most long-lived proteins and entire organelles. Autophagy participates in some reproductive events; however, there are not reports regarding the role of autophagy in the regulation of sperm physiology. Hence, the aim of this study was to investigate whether autophagy-related proteins are present and functionally active in human spermatozoa. Proteins related to autophagy/mitophagy process (LC3, Atg5, Atg16, Beclin 1, p62, m-TOR, AMPKα 1/2, and PINK1) were present in human spermatozoa. LC3 colocalized with p62 in the middle piece of the spermatozoa. Autophagy activation induced a significant increase in motility and a decrease in PINK1, TOM20 expression and caspase 3/7 activation. In contrast, autophagy inhibition resulted in decreased motility, viability, ATP and intracellular calcium concentration whereas PINK1, TOM20 expression, AMPK phosphorylation and caspase 3/7 activation were significantly increased. In conclusion our results show that autophagy related proteins and upstream regulators are present and functional in human spermatozoa. Modification of mitochondrial proteins expression after autophagy activation/inhibition may be indicating that a specialized form of autophagy named mitophagy may be regulating sperm function such as motility and viability and may be cooperating with apoptosis.

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Section: Discussionsupporting

confidence: 93%

Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility

Aparicio

Espino

Bejarano

et al. 2016

Sci Rep

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“…In a previous study, we showed that U. chamae contained phytochemicals like alkaloids and tannins among others (Enin et al, 2021). Alkaloids have been shown to decrease motility (Trifunac and Bernstein, 1982). In that study, an antimalarial agent (chloroquine) was found to impair motility just like in this current study.…”

Section: Discussionsupporting

confidence: 77%

High doses of Uvaria chamae root extract impair sperm motility and viability

Ujah¹,

Busayo²,

Emeng³

et al. 2021

Lekovite sirovine

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Uvaria chamae is a tropical medicinal plant with documented antimicrobial and antimalarial activities. Agents with these activities are known to impair fertility. This study investigated the antifertility effects of ethanolic extract of U. chamae on male albino Wistar rats. Two different oral doses; 56.6 mg/kg body weight and 113.2 mg/kg body weight of the extracts were administered to low dose and high dose group, respectively. A third group served as control and received 2 mL of distilled water. There were five animals in each group. Administration lasted six weeks after which animals were sacrificed and samples collected. Results showed that sperm count, sperm morphology and semen pH were not affected (P>0.05) by the extract. Sperm motility (55.65 %) and viability (64.38 %) were however decreased (P<0.05) by high doses of the extract. Histopathology of testicular tissues also showed no difference across the groups. U. chamae thus impair fertility in vivo especially at high doses.

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“…It is a known K + channel blocker and is most widely known for its use treating malaria. However, the effects of quinine on spermatozoa have been studied as far back as the 1980s .…”

Section: Results and Discussionmentioning

confidence: 99%

“…However, the effects of quinine on spermatozoa have been studied as far back as the 1980s. 79 In vivo studies: Yeung et al have suggested that this mechanism may be due to effects on sperm volume regulation where swelling of spermatozoa leads to angulation of the tail, resulting in failure of spermatozoa to pass through the utero-tubal junction in the female reproductive tract for fertilization. 80 C-ros knockout mice are infertile and display angulation of the tail due to swelling of spermatozoa.…”

Section: ■ Methodsmentioning

confidence: 99%

Natural Products with Potential for Nonhormonal Male Contraception

2021

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Despite increased access to contraception over the last 60 years, unplanned pregnancies continue to contribute to economic disparities and overpopulation. Additionally, the burden of family planning falls primarily on women, as a reliable pharmaceutical male contraceptive has yet to be developed. The objective of this literature-based systematic review was to identify compounds for future study from natural sources with potential nonhormonal male contraceptive activity. After the exclusion of extracts and compounds with known hormonal mechanisms, 26 unique compounds were identified from natural species. The plant source, compound class, structure, target, mechanism of action, safety/toxicity profile, and in vitro, in vivo, and human studies for each compound were evaluated and discussed. β-Caryophyllene, embelin, oleanolic acid, triptonide, and N-butyldeoxynojirimycin (NB-DNJ) were selected as the five most promising compounds for future study using prespecified criteria such as number of studies, efficacy and safety profile, reversibility, and previous use in humans for any indication. In order to move forward with development of a male contraceptive from a natural source, additional studies are needed to determine the predicted safety and efficacy for in vivo and human clinical trials.

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Inhibition of the metabolism and motility of human spermatozoa by various alkaloids

Cited by 9 publications

References 14 publications

Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility

Autophagy-related proteins are functionally active in human spermatozoa and may be involved in the regulation of cell survival and motility

High doses of Uvaria chamae root extract impair sperm motility and viability

Natural Products with Potential for Nonhormonal Male Contraception

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