Inhibition of the essential oil from Chenopodium ambrosioides L. and α-terpinene on the NorA efflux-pump of Staphylococcus aureus

Oliveira-Tintino, Cícera Datiane de Morais; Tintino, Saulo Relison; Limaverde, Paulo W.; Figueredo, Fernando Gomes; Campina, Fábia F.; Cunha, Francisco Assis Bezerra da; Costa, Roger Henrique Sousa da; Pereira, Pedro Silvino; Lima, Luciene Ferreira de; Matos, Yedda Maria Lobo Soares de; Coutinho, Henrique Douglas Melo; Siqueira-Júnior, José P.; Balbino, Valdir Queiroz; Silva, Teresinha Gonçalves da

doi:10.1016/j.foodchem.2018.04.040

Cited by 59 publications

(28 citation statements)

References 32 publications

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“…ose factors compromised all or third-generation cephalosporins and penicillin classes [15]. Overall, those factors contributed to resisted amoxicillin, tetracycline, piperacillin, ofloxacin, and oxacillin [16,18,20].…”

Section: Discussionmentioning

confidence: 99%

“…In such case, essential oils are suitable candidates to develop topical ointments for wound care and beyond [9]. Another study demonstrated that EOs caused sequential inhibition of biochemical pathway, inhibit protein synthesis, and disintegrated the outer cell membrane [18,28]. Overall, essential oils have compounds that used to produce affordable and safe antibiotics and potentially activate the immune system for fast wound healing.…”

Section: Discussionmentioning

confidence: 99%

“…Another study showed that EOs of Pilgerodendron uviferum and Melaleuca alternifolia can inhibit efflux pump mechanism of the S. aureus NorA. ese essential oils can inhibit efflux pump and characterize a potentially safe and affordable ingredients to develop skin friendly ointment to wound colonizing multidrug-resistant bacteria [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Antibacterial Activity of Essential Oils and Their Combination against Multidrug-Resistant Bacteria Isolated from Skin Ulcer

Gadisa¹,

Usman

2021

International Journal of Microbiology

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Background. Emerging of multidrug-resistant bacteria can compromise the effectiveness of antibiotics used to treat skin infections. Those bacteria imposed public health problems and questioning medical care in the 21st century. In this circumstance, essential oils of medicinal plants origin are supreme sources of structural and functionally divergent compounds, which inhibited the growth of common wound colonizing MRSA and ESBL producing P. aeruginosa. The aim of this study was to evaluate the combined antibacterial activity of essential oils extracted from Rumex abyssinicus, Cucumis pustulatus, and Discopodium penninervium against multidrug-resistant (MDR) isolates of skin ulcers. Methods. Essential oils (EOs) were extracted from aerial parts of R. abyssinicus, C. pustulatus, and D. penninervium with steam distillation. A mixture of each oil (1 : 1) was adsorbed to a disc and placed on Mueller Hinton Agar. Then, minimum zone of inhibition and bactericidal concentration of EOs was measured after incubeted for 18–24 hours at 37 °C. Their combined antibacterial effect was determined by the fractional inhibitory concentration index. Results. The antibacterial activity of mixed oil varied in their doses and bacteria species, of which a mixture of essential oil of R. abyssinicus and D. penninervium had inhibition zone (32 mm); its MIC and MBC values range from 1-2 μl/ml against MRSA. It had an inhibition zone (36 mm), MIC value 4 μl/ml, and MBC (8 μl/ml) against ESBL producing P. aeruginosa, whereas combined effects of R. abyssinicus and C. pustulatus had MIC values ranging from 2–8 μl/ml for E. coli and K. pneumoniae and 2 μl/ml for MRSA. There was a strong synergistic effect between R. abyssinicus and D. penninervium and promising antibacterial effect more specifically on MRSA and P. aeruginosa. Conclusion. This in vitro study of the combined effect of EOs has significant antibacterial activity on wound colonizing bacteria and reduces delaying wound healing as that of modern drugs tested in parallel. Hence, further structural elucidation of active compounds helps us to properly design or synthesis of topical antibiotics for wound care.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Antibacterial Activity of Essential Oils and Their Combination against Multidrug-Resistant Bacteria Isolated from Skin Ulcer

Gadisa¹,

Usman

2021

International Journal of Microbiology

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“…Accordingly, Oliveira-Tintino et al [40] reported that while the essential oil of Chenopodium ambrosioides significantly reduced the MIC of ethidium bromide against the 1199B strain, its principal constituent α-Terpinene presented no significant modulating effect, suggesting that other components of the C. ambrosioides oil could act as EP inhibitors. However, the molecular mechanisms associated with EP inhibition by essential oils, as well as their isolated components, remain to be fully characterized.…”

Section: Discussionmentioning

confidence: 99%

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

et al. 2020

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Considering the evidence that essential oils, as well as safrole, could modulate bacterial growth in different resistant strains, this study aims to characterize the phytochemical profile and evaluate the antibacterial and antibiotic-modulating properties of the essential oil Ocotea odorífera (EOOO) and safrole against efflux pump (EP)-carrying strains. The EOOO was extracted by hydrodistillation, and the phytochemical analysis was performed by gas chromatography coupled to mass spectrometry (GC-MS). The antibacterial and antibiotic-modulating activities of the EOOO and safrole against resistant strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were analyzed through the broth microdilution method. The EP-inhibiting potential of safrole in association with ethidium bromide or antibiotics was evaluated using the S. aureus 1199B and K2068 strains, which carry genes encoding efflux proteins associated with antibiotic resistance to norfloxacin and ciprofloxacin, respectively. A reduction in the MIC of ethidium bromide or antibiotics was used as a parameter of EP inhibition. The phytochemical analysis identified 16 different compounds in the EOOO including safrole as the principal constituent. While the EOOO and safrole exerted clinically relevant antibacterial effects against S. aureus only, they potentiated the antibacterial activity of norfloxacin against all strains evaluated by our study. The ethidium bromide and antibiotic assays using the strains of S. aureus SA1119B and K2068, as well as molecular docking analysis, indicated that safrole inhibits the NorA and MepA efflux pumps in S. aureus. In conclusion, Ocotea odorifera and safrole presented promising antibacterial and antibiotic-enhancing properties, which should be explored in the development of drugs to combat antibacterial resistance, especially in strains bearing genes encoding efflux proteins.

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“…α-Terpinene was considered a component responsible for the trypanocidal effect in Melaleuca alternifolia essential oil with known efficacy in the treatment of trypanosomosis [18]. α-Terpinene enhanced the antibiotic activity of the essential oil from Chenopodium ambrosioides against S. aureus strains [29]. In this study, α-terpinene showed a direct inhibitive effect on the selected pathogenic fungi (Table 3).…”

Section: Discussionmentioning

confidence: 57%

An Endophytic Diaporthe apiculatum Produces Monoterpenes with Inhibitory Activity against Phytopathogenic Fungi

et al. 2019

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Volatile organic compounds (VOCs) from endophytic fungi are becoming a potential antibiotic resource. The inhibitive effects of VOCs produced by an endophytic fungus in Leucaena leucocephala were investigated on plant pathogens in this study. Using standard morphological methods and multigene phylogeny, the fungus was identified as Diaporthe apiculatum strain FPYF 3052. Utilizing a two- compartment Petri plate bioassay method, the VOCs from this fungus showed bioactivity ranging from 23.8% to 66.7% inhibition on eight plant pathogens within 24 hours. The SPME-GC/MS technique identified fifteen volatile compounds with dominant terpenoids γ-terpinene (39.8%), α-terpinene (17.2%), and (-)-4-terpineol (8.4%) from the VOCs. Commercial α-terpinene, γ-terpinene, and (-)-4-terpineol demonstrated inhibition on the tested pathogens at concentrations from 0.2 to 1.0 µl/ml within 72 h in the bioassay system. The inhibition rates were from 28% to 100% percent using 1.0 µl/ml within 48 h. (-)-4-Terpineol was the most active of the terpenoids causing up to 100% inhibition. The data illustrate that these monoterpenes play an important role in the inhibitive bioactivity of the VOCs of D. apiculatum FPYF 3052. Most importantly, (-)-4-terpineol is now for the first time, reported to have capability of strong antifungal activity and could be developed as an antibiotic substance.

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Inhibition of the essential oil from Chenopodium ambrosioides L. and α-terpinene on the NorA efflux-pump of Staphylococcus aureus

Cited by 59 publications

References 32 publications

Evaluation of Antibacterial Activity of Essential Oils and Their Combination against Multidrug-Resistant Bacteria Isolated from Skin Ulcer

Evaluation of Antibacterial Activity of Essential Oils and Their Combination against Multidrug-Resistant Bacteria Isolated from Skin Ulcer

GC-MS Profile and Enhancement of Antibiotic Activity by the Essential Oil of Ocotea odorífera and Safrole: Inhibition of Staphylococcus aureus Efflux Pumps

An Endophytic Diaporthe apiculatum Produces Monoterpenes with Inhibitory Activity against Phytopathogenic Fungi

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