Inhibition of the epidermal growth factor receptor by erlotinib prevents immortalization of human cervical cells by Human Papillomavirus type 16

Woodworth, Craig D.; Diefendorf, Laura P.; Jette, David; Mohammed, Abdulmajid; Moses, Michael A.; Searleman, Sylvia A.; Stevens, Dan A.; Wilton, Katelynn M.; Mondal, Sumona

doi:10.1016/j.virol.2011.09.014

Cited by 24 publications

(18 citation statements)

References 41 publications

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“…CXT cervical cancer cell lines and normal human cervical epithelial cells were isolated and cultured from tissue purchased from the Cooperative Human Tissue Network as described elsewhere [28]. Normal and CXT cancerous cells were cultured in Keratinocyte Serum‐Free Medium (KSFM) supplemented with 50 units/mL penicillin G, and 50 μg/mL streptomycin.…”

Section: Methodsmentioning

confidence: 99%

Selective permeabilization of cervical cancer cells to an ionic DNA‐binding cytotoxin by activation of P2Y receptors

et al. 2015

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Extracellular ATP is known to permeabilize certain cell types to polyatomic cations like YO-PRO1. Here, we report that extracellularly applied ATP stimulated rapid uptake and accumulation of an otherwise weakly membrane permeable fluorescent DNA-binding cytotoxin, Hoechst 33258, into cervical cancer cells. While ATP stimulated Hoechst 332uptake in 20–70% of cells from seven cervical cancer cell lines, it consistently stimulated uptake in less than 8% of cervical epithelial cells obtained from the normal transformation zone and ectocervix tissue of 10 patients. ATP-evoked Hoechst 33258 uptake was independent of ionotropic P2X receptors, but dependent on activation of P2Y receptors. Thus, we show here that cervical cancer cells can be selectively induced to take up and accumulate an ionic cytotoxin by exposure to extracellular ATP.

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Section: Methodsmentioning

confidence: 99%

Selective permeabilization of cervical cancer cells to an ionic DNA‐binding cytotoxin by activation of P2Y receptors

et al. 2015

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“…The 3 early expression oncoproteins E5, E6, and E7 of highrisk HPV have complex interactions with the EGFR signaling pathway. The HPV-16 E6 and E7 oncoproteins 22,23 It has been shown that the disruption of EGFR inhibited development of papilloma and carcinoma from immortalized epithelial cells in mice. 9,24 Thus, the EGFR pathway is an important molecular target in cervical cancer treatment.…”

Section: Discussionmentioning

confidence: 99%

EGFR Promoter Methylation, EGFR Mutation, and HPV Infection in Chinese Cervical Squamous Cell Carcinoma

Zhang¹,

Jiang²,

et al. 2015

Applied Immunohistochemistry &Amp; Molecular Morphology

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Therapy strategy toward epidermal growth factor receptor (EGFR) inhibition in cervical cancer has been ongoing. EGFR promoter methylation status and EGFR tyrosine kinase inhibitor-sensitive mutations in cervical cancer may be significant for clinical outcome prediction using anti-EGFR treatment. In this study, EGFR tyrosine kinase inhibitor-sensitive mutations, EGFR exons 18, 19, and 21 mutations, were detected by sequencing in a total of 293 Chinese cervical squamous cell carcinoma tissue samples. EGFR promoter methylation status was detected by an EGFR asymmetric PCR and hybridization-fluorescence polarization assay and sequencing in 293 Chinese cervical squamous cell carcinoma tissue samples. High-risk human papillomavirus (HPV) genotypes in 293 Chinese cervical squamous cell carcinoma tissue samples were detected by an asymmetric GP5+/6+ PCR and hybridization-fluorescence polarization assay. No EGFR exons 18, 19, and 21 mutations were detected, EGFR promoter methylation status was identified in 98 samples, and HPV 16 infection was the first frequent HPV genotype. The methylated EGFR promoter was identified most frequently in cervical squamous cell carcinoma samples with HPV 16 infection (53.4%). Statistical significant difference of EGFR promoter methylation prevalence was found between HPV 16 and other HPV genotypes (P<0.01). This study suggested that there was no EGFR tyrosine kinase inhibitor-sensitive mutation in EGFR exons 18, 19, and 21 in Chinese cervical squamous cell carcinoma tissue samples. EGFR promoter methylation was common and it might be associated with HPV 16 infection in Chinese cervical squamous cell carcinoma. The results provided a novel understanding and an applicable pharmacogenomic tool for individualized management of cervical cancer patients.

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“…2). EGFR signaling is also needed for immortalization by either the HPV16 genome or E6/E7 alone 126 . The primary HPV protein that regulates EGFR signaling is E5.…”

Section: Growth Factorsmentioning

confidence: 99%

The Interaction Between Human Papillomaviruses and the Stromal Microenvironment

Woodby

Scott

Bodily

2016

Progress in Molecular Biology and Translational Science

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Human papillomaviruses (HPV) are small, double-stranded DNA viruses that replicate in stratified squamous epithelia and cause a variety of malignancies. Current efforts in HPV biology are focused on understanding the virus-host interactions that enable HPV to persist for years or decades in the tissue. The importance of interactions between tumor cells and the stromal microenvironment has become increasingly apparent in recent years, but how stromal interactions impact the normal, benign life cycle of HPVs or progression of lesions to cancer is less understood. Furthermore, how productively replicating HPV impacts cells in the stromal environment is also unclear. Here we bring together some of the relevant literature on keratinocyte-stromal interactions and their impacts on HPV biology. We discuss how HPV oncogenes in infected cells manipulate other cells in their environment, and, conversely, how neighboring cells may impact the efficiency or course of HPV infection.

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Inhibition of the epidermal growth factor receptor by erlotinib prevents immortalization of human cervical cells by Human Papillomavirus type 16

Cited by 24 publications

References 41 publications

Selective permeabilization of cervical cancer cells to an ionic DNA‐binding cytotoxin by activation of P2Y receptors

Selective permeabilization of cervical cancer cells to an ionic DNA‐binding cytotoxin by activation of P2Y receptors

EGFR Promoter Methylation, EGFR Mutation, and HPV Infection in Chinese Cervical Squamous Cell Carcinoma

The Interaction Between Human Papillomaviruses and the Stromal Microenvironment

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