Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3

Kimoto, Mai; Kitagawa, Tsuyuki; Kobayashi, Isao; Nakata, Tomohiro; Kuroiwa, Asato; Takiya, Shigeharu

doi:10.1007/s00427-012-0418-4

Cited by 28 publications

(21 citation statements)

References 37 publications

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“…fhxh4/ fhxh5 expression was confined to the middle and posterior portions of the MSG, and this expression shows a good correlation with the territories in which the MIC is formed (23). In addition to transcriptional activation by MIC, a POU-type transcription factor, POU-M1, might act to repress fhxh4/fhxh5 in the MSG-A and/or in the MSG-M; its role in ser1 repression has already been demonstrated (20). ser3 is normally expressed in the MSG-A/MSG-M (Fig.…”

Section: Discussionmentioning

confidence: 96%

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori

Tsubota

Tomita

Uchino

et al. 2016

Journal of Biological Chemistry

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Hox genes play a pivotal role in the determination of anteroposterior axis specificity during bilaterian animal development. They do so by acting as a master control and regulating the expression of genes important for development. Recently, however, we showed that Hox genes can also function in terminally differentiated tissue of the lepidopteran Bombyx mori. In this species, Antennapedia (Antp) regulates expression of sericin-1, a major silk protein gene, in the silk gland. Here, we investigated whether Antp can regulate expression of multiple genes in this tissue. By means of proteomic, RT-PCR, and in situ hybridization analyses, we demonstrate that misexpression of Antp in the posterior silk gland induced ectopic expression of major silk protein genes such as sericin-3, fhxh4, and fhxh5. These genes are normally expressed specifically in the middle silk gland as is Antp. Therefore, the evidence strongly suggests that Antp activates these silk protein genes in the middle silk gland. The putative sericin-1 activator complex (middle silk gland-intermoltspecific complex) can bind to the upstream regions of these genes, suggesting that Antp directly activates their expression. We also found that the pattern of gene expression was well conserved between B. mori and the wild species Bombyx mandarina, indicating that the gene regulation mechanism identified here is an evolutionarily conserved mechanism and not an artifact of the domestication of B. mori. We suggest that Hox genes have a role as a master control in terminally differentiated tissues, possibly acting as a primary regulator for a range of physiological processes.

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Section: Discussionmentioning

confidence: 96%

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori

Tsubota

Tomita

Uchino

et al. 2016

Journal of Biological Chemistry

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“…However, thus far, only SGF3/POU‐M1, which binds to the C, D and E sites upstream of fibH , has been proven to suppress fibH expression in the anterior regions of the silk gland (Hui et al ., ; Takiya et al ., ; Matsunami et al ., ). However, the expression level of POU‐M1 is low in the PSG (Kimoto et al ., ). By contrast, the MBF2 protein shows a high expression level in the PSG from day 3 of the fourth instar to the beginning of the fifth instar, and its expression ceases by the end of the fifth instar.…”

Section: Discussionmentioning

confidence: 97%

Multiprotein bridging factor 2 regulates the expression of the fibroin heavy chain gene by interacting with Bmdimmed in the silkworm Bombyx mori

Zhou

Zha

Shi

et al. 2016

Insect Molecular Biology

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Multiprotein bridging factor 2 (MBF2) was first isolated from the posterior silk gland of Bombyx mori. However, its function in B. mori is still unknown. Herein, MBF2 transcripts were detected mainly in the posterior silk gland and Malpighian tubules of B. mori larvae via a quantitative PCR analysis. An analysis of temporal expression patterns showed that the expression pattern of MBF2 was the opposite of that of the fibroin heavy chain (fibH) gene, as its expression was high during the fourth-instar moulting stage, decreased gradually during the fifth-instar feeding stage and disappeared at the end of the fifth-instar phase. Furthermore, bimolecular fluorescent complementation and Far-Western blot assays showed that MBF2 interacted with the basic helix-loop-helix transcription factor Bmdimmed. Dual luciferase reporter assays showed that MBF2 down-regulated the promoter activity of fibH and inhibited the effect of Bmdimmed (Bmdimm) on fibH expression. MBF2 expression was induced in silk glands after treatment with 20-hydroxyecdysone in vivo and in vitro. These findings suggest that MBF2 is a transcriptional repressor that is involved in controlling the regulation of the fibH gene in the posterior silk gland by interacting with Bmdimm.

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“…However, we hypothesize that the fast and fierce 20E induction of BmCYP18A1 by 20E is important for MSG degeneration after spinning. Bombyx mori silk glands are fully formed during embryogenesis but grow enormously in size without mitosis during the final larval stage (Dhawan and Gopinathan, 2003; Kimoto et al, 2012). At this time, massive accumulation of silk protein is provoked by activation of insulin pathway (Wang et al, 2009b), repressing TOR transcription and inhibition of 20E signal transduction (Delanoue et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

CYP18A1 regulates tissue-specific steroid hormone inactivation in Bombyx mori

Lin

et al. 2014

Insect Biochemistry and Molecular Biology

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Insect development and metamorphosis are regulated by two major hormones, juvenile hormone and ecdysteroids. Despite being the key regulator of insect developmental transitions, the metabolic pathway of the primary steroid hormone, 20-hydroxyecdysone (20E), especially its inactivation pathway, is still not completely elucidated. A cytochrome P450 enzyme, CYP18A1, has been shown to play key roles in insect steroid hormone inactivation through 26-hydroxylation. Here, we identified two CYP18 (BmCYP18A1 and BmCYP18B1) orthologs in the lepidopteran model insect, Bombyx mori. Interestingly, BmCYP18A1 gene is predominantly expressed in the middle silk gland (MSG) while BmCYP18B1 expresses ubiquitously in B. mori. BmCYP18A1 is induced by 20E in vitro, suggesting its role in 20E metabolism. Using the binary Gal4/UAS transgenic system, we ectopically overexpressed BmCYP18A1 in a MSG-specific manner with a Sericin1-Gal4 (Ser-Gal4) driver or in a ubiquitous manner with an Actin3-Gal4 (A3-Gal4) driver. Ectopic overexpression of BmCYP18A1 in MSG or in all tissues resulted in developmental arrestment of transgenic animals during the final instar larval stage. The 20E titers in the transgenic animals expressing BmCYP18A1 were lower compared to the levels in the control animals. Although the biological significance of MSG-specific expression of BmCYP18A1 is unclear, our results provide the first evidence that BmCYP18A1, which is conserved in most arthropods, is involved in a tissue-specific steroid hormone inactivation in B. mori.

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Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3

Cited by 28 publications

References 37 publications

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori

Multiprotein bridging factor 2 regulates the expression of the fibroin heavy chain gene by interacting with Bmdimmed in the silkworm Bombyx mori

CYP18A1 regulates tissue-specific steroid hormone inactivation in Bombyx mori

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