Inhibition of the Aspergillus flavus Growth and Aflatoxin B1 Contamination on Pistachio Nut by Fengycin and Surfactin-Producing Bacillus subtilis UTBSP1

Farzaneh, Mohsen; Shi, Zhiqi; Ahmadzadeh, Masoud; Hu, Liangbin; Ghassempour, Alireza

doi:10.5423/ppj.oa.11.2015.0250

Cited by 49 publications

(31 citation statements)

References 35 publications

(60 reference statements)

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“…also appeared as valuable candidates for controlling filamentous fungal growth and inhibiting mycotoxin production [ 14 ]. Bacillus subtilis UTBSP1 can effectively restrict the growth of A. flavus growth and remove AFB 1 in pistachio nut as previously described by Farzaneh et al [ 21 ], while 67.2% AFB 1 degradation by cell-free supernatant of Bacillus subtilis JSW-1 was also observed by Xia et al [ 14 ]. Raksha et al [ 22 ] reported that Bacillus licheniformis CFR1 can reduce AFB 1 by 94.7% and eliminate the AFB 1 induced mutagenicity, indicating that Bacillus spp.…”

Section: Introductionmentioning

confidence: 59%

Biological Degradation of Aflatoxin B1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability

Shu

Wang

Zhou

et al. 2018

Toxins

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(1) Background: Aflatoxin contamination in food and grain poses serious problems both for economic development and public health protection, thus leading to a focus on an effective approach to control it; (2) Methods: Aflatoxin B1 (AFB1) degrading bacteria were isolated using a medium containing coumarin as the sole carbon source, and the biodegradation of AFB1 by the isolate was examined by high performance liquid chromatography, and liquid chromatography mass spectrometry; (3) Results: a bacterial strain exhibiting strong AFB1 degradation activity (91.5%) was isolated and identified as Bacillus velezensis DY3108. The AFB1 degrading activity was predominantly attributed to the cell-free supernatant of strain DY3108. Besides, it was heat-stable and resistant to proteinase K treatment but sensitive to sodium dodecyl sulfate treatment. The optimal temperature for the maximal degradation of AFB1 was 80 °C. Even more notable, the supernatant showed a high level of activity over a broad pH (4.0 to 11.0) and exhibited the highest degradation (94.70%) at pH 8.0. Cytotoxicity assays indicated that the degradation products displayed significantly (p < 0.05) lower cytotoxic effects than the parent AFB1; (4) Conclusions: B. velezensis DY3108 might be a promising candidate for exploitation in AFB1 detoxification and bioremediation in food and feed matrices.

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Section: Introductionmentioning

confidence: 59%

Biological Degradation of Aflatoxin B1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability

Shu

Wang

Zhou

et al. 2018

Toxins

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“…Application of atoxigenic A. flavus strains was shown to reduce aflatoxin contamination by toxigenic A. flavus in maize, cottonseed and groundnuts [13,17]. Several mechanisms have been implicated in the efficacy of bio-control agents with either competitive exclusion of toxigenic A. flavus by atoxigenic ones or biosynthesis of antifungal compounds, that inhibit or completely arrest growth of mycotoxin-producing fungi [13,29,30]. The ability of fungi to colonize crops, survive and produce toxins is also affected by a range of environmental conditions that include temperature, rainfall and relative humidity [29,31].…”

Section: Introductionmentioning

confidence: 99%

Variation in Occurrence and Aflatoxigenicity of Aspergillus flavus from Two Climatically Varied Regions in Kenya

Monda

Masanga

Alakonya

2020

Toxins

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Aflatoxins are carcinogenic chemical metabolites produced by Aspergillus spp. of the section Flavi. In Kenya, Aspergillus flavus is the most prevalent and has been associated with several acute and chronic aflatoxin outbreaks in the past. In this study, we evaluated the occurrence of A. flavus in soils from two agro-ecological regions with contrasting climatic conditions, aflatoxin contamination histories and cropping systems. Aspergillus spp. were first isolated from soils before the identification and determination of their aflatoxigenicity. Further, we determined the occurrence of Pseudomonas and Bacillus spp. in soils from the two regions. These bacterial species have long been associated with biological control of several plant pathogens including Aspergillus spp. Our results show that A. flavus occurred widely and produced comparatively higher total aflatoxin levels in all (100%) study sites from the eastern to the western regions of Kenya. For the western region, A. flavus was detected in 4 locations (66.7%) that were previously under maize cultivation with the isolates showing low aflatoxigenicity. A. flavus was not isolated from soils under sugarcane cultivation. Distribution of the two bacterial species varied across the regions but we detected a weak relationship between occurrence of bacterial species and A. flavus. We discuss these findings in the context of the influence of climate, microbial profiles, cropping systems and applicability in the deployment of biological control remedies against aflatoxin contamination.

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“…B. subtilis produces a variety of antibiotic substances, including iturine and fengycin, which are inhibitory to Fusarium, Penicillium, Aspergillus, Colletotrichum, and Rhizoctonia solani (Nagórska, Bikowski, & Obuchowski, 2007); surfactin, which has synergistic effects with fengycin against A. flavus (Farzaneh, Shi, Ahmadzadeh, Hu, & Ghassempour, 2016); and bacillomycin, a compound with fungicidal activity (Gong et al, 2014). P. fluorescens produces 2,4-diacetylphloroglucinol, a prominent antimicrobial that inhibits the growth of several phytopathogenic bacteria, oomycetes, and fungi (Couillerot et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Combined Effects of Biological and Chemical Treatment on Rice Seed Physiological and Sanitary Quality

Pereira¹,

Benites²,

Catelan³

et al. 2019

JAS

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This study aimed to evaluate the efficiency of integrated biological and chemical control of pathogens in rice seeds and their effects on seed quality. The experiment was conducted in a 2 × 5 factorial completely randomized design. Fungicide-treated (carboxin/thiram) and untreated rice seeds were inoculated with distilled water (control), Azospirillum brasilense, Bacillus subtilis, Pseudomonas fluorescens, or Trichoderma harzianum. Seed vigor and viability, shoot and root length, and seedling dry weight were determined. The blotter test was carried out to assess seed health. Fungicide treatment improved seed vigor and viability and reduced the incidence of fungi. Biological treatment did not enhance the physiological quality of seeds but was able to control fungi. A. brasilense, B. subtilis, P. fluorescens, and T. harzianum controlled Phoma sorghina; B. subtilis, P. fluorescens, and T. harzianum were effective against Aspergillus flavus; P. fluorescens and T. harzianum controlled Pyricularia oryzae; and T. harzianum was effective against Gerlachia oryzae.

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Inhibition of the Aspergillus flavus Growth and Aflatoxin B1 Contamination on Pistachio Nut by Fengycin and Surfactin-Producing Bacillus subtilis UTBSP1

Cited by 49 publications

References 35 publications

Biological Degradation of Aflatoxin B1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability

Biological Degradation of Aflatoxin B1 by Cell-Free Extracts of Bacillus velezensis DY3108 with Broad PH Stability and Excellent Thermostability

Variation in Occurrence and Aflatoxigenicity of Aspergillus flavus from Two Climatically Varied Regions in Kenya

Combined Effects of Biological and Chemical Treatment on Rice Seed Physiological and Sanitary Quality

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