Inhibition of the Anti‐Globulin Reaction by Nonspecifically Cell‐Bound Human γG‐Immunoglobulin

A B S T R A C Texhibit Rh specificity and did not react with nonprimate red cells. When studied by sucrose gradient ultracentrifugation, the vG antibodies to human red cells in these eluates sedimented in the 7S region. It is concluded that in many patients in whom direct antiglobulin tests reveal only cell-bound complement, the complement fixation is mediated in vivo by small quantities of "warmreacting" erythrocyte autoantibodies of the aG class.

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The detection of cell-bound antibody on complement-coated human red cells

Gilliland¹,

Leddy²,

Vaughan³

1970

J. Clin. Invest.

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Inhibitory Effects of Monoclonal Immunoglobulins on Anti-globulin Reaction

Itoh

Hashimoto

Onuma

et al. 1968

Tohoku J. Exp. Med.

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Specific monoclonal immunoglobulins (Igs) were purified from myeloma sera and urines, and the proteins were submitted to physicochemical treatments including ultrasonic irradiation, heat aggregation and reduction by mercaptoethanol. By using these protein preparations, their inhibitory effects upon the agglutination of anti-D sensitized erythrocytes with anti-globulin serum (anti-globulin reaction) were investigated.Some of the monoclonal IgGs were found to exert no inhibitory action and were not fixed onto cells. Monoclonal Igs other than IgG did not inhibit the anti globulin reaction (AGR).Neither ultrasonic irradiation nor heat aggregation altered the inhibitory activity of monoclonal IgG, whereas a little loss of inhibitory activity was observed in cases of reduction of IgG. These results clearly indicate that the anti-D antibody is of the IgG type.Neuraminidase treatment of erythrocytes and the presence of red cell stromal substance (RCSS) in the reaction mixture disturbed the inhibitory activity of IgG.

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Inhibition of the Anti‐Globulin Reaction by Nonspecifically Cell‐Bound Human γG‐Immunoglobulin

Cited by 2 publications

References 8 publications

The detection of cell-bound antibody on complement-coated human red cells

The detection of cell-bound antibody on complement-coated human red cells

Inhibitory Effects of Monoclonal Immunoglobulins on Anti-globulin Reaction

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