2000
DOI: 10.1006/bbrc.2000.2419
|View full text |Cite
|
Sign up to set email alerts
|

Inhibition of the 26S Proteasome Induces Expression of GLCLC, the Catalytic Subunit for γ-Glutamylcysteine Synthetase

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

5
30
0

Year Published

2001
2001
2013
2013

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations
Cited by 46 publications
(35 citation statements)
references
References 34 publications
5
30
0
Order By: Relevance
“…ARE-dependent ␥-gcs gene expression is regulated, in part, by the "cap'n'collar" family of basic leucine zipper transcription factor Nrf2 (41,42). Previous studies have demonstrated that Nrf2 is degraded by proteasomes, and proteasome inhibition induces the accumulation of Nrf2 and ARE activation, resulting in ␥-gcs catalytic gene expression (43)(44)(45). Corresponding to these reports, we showed that Nrf2 translocated to the nucleus and that ␥-GCS mRNA expression was significantly increased after exposure to lactacystin (Fig.…”
Section: Discussionsupporting
confidence: 81%
“…ARE-dependent ␥-gcs gene expression is regulated, in part, by the "cap'n'collar" family of basic leucine zipper transcription factor Nrf2 (41,42). Previous studies have demonstrated that Nrf2 is degraded by proteasomes, and proteasome inhibition induces the accumulation of Nrf2 and ARE activation, resulting in ␥-gcs catalytic gene expression (43)(44)(45). Corresponding to these reports, we showed that Nrf2 translocated to the nucleus and that ␥-GCS mRNA expression was significantly increased after exposure to lactacystin (Fig.…”
Section: Discussionsupporting
confidence: 81%
“…Inhibition of the proteasomal degradation of Nrf2 blocks the cycle, leading to the accumulation of the Keap1-Nrf2 complex in the closed conformation. Inducers function to inhibit the ubiquitination of Nrf2, but crucially, in the absence of new translation, Nrf2 is not stabilized in the induced state, and Nrf2-target genes are not upregulated (24,25), suggesting it is the de novo translated Nrf2 which translocates to the nucleus, whereas the existing population of Nrf2 remains bound to Keap1.…”
Section: Discussionmentioning
confidence: 99%
“…Nrf2 is a substrate for the ubiquitin-proteasome system. Treatment of cells with a proteasome inhibitor prevents rapid degradation of this protein, leading to enhanced expression of the downstream gene encoding ␥-glutamylcysteine ligase (37). Recently, Itoh et al (21) proposed that Nrf2 protein turnover is regulated by Keap1-mediated subcellular compartmentalization of this transcription factor.…”
Section: Discussionmentioning
confidence: 99%