Inhibition of Tanshinone IIA, Salvianolic Acid A and Salvianolic Acid B on Areca Nut Extract-Induced Oral Submucous Fibrosis in Vitro

Dai, Jinghong; Zhu, Dan Xia; Sheng, Jiang; Chen, Xiao Xuan; Li, Wei Zhong; Wang, Ge Fei; Li, KS; Su, Yun

doi:10.3390/molecules20046794

Cited by 31 publications

(24 citation statements)

References 33 publications

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“…However, many in vitro studies have indicated that ANE increases collagen formation via the transdifferentiation of myofibroblasts that express the intracellular marker protein alpha smooth muscle actin (α-SMA) and further decrease collagen degradation via the modulation of matrix metalloproteinase (MMP) activity [13–15]. Furthermore, ANE may induce fibrosis through transforming growth factor-beta (TGF-β), connective tissue growth factor (CTGF), interleukin 6 (IL-6), and prostaglandin E2 (PGE2) signaling factors in different cell lines.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Dermal Fibrosis Model Induced by Areca Nut Extract that Mimics Oral Submucous Fibrosis

Chiang

Chen

et al. 2016

PLoS ONE

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Oral submucous fibrosis (OSF) is an oral potentially malignant disorder and areca quid chewing is the main etiological factor. However, the molecular mechanism underlying OSF remains unclear, partly due to the lack of an appropriate animal model. The present study aimed to establish and characterize an animal model of areca nut extract (ANE)-induced skin fibrosis that mimics OSF. Mice were divided into 4 groups: the control group; the bleomycin group; and the ANE10 and ANE20 groups, which received 10mg/ml and 20mg/ml subcutaneous (SC) injection of ANE, respectively. Skin fibrosis was evaluated by histological analyses. Additionally, the expression levels of the fibrotic marker genes were determined by immunohistochemical staining and immunoblotting. ANE administration significantly increased dermal thickness and collagen deposition compared with the control group. Moreover, ANE induced the expression of the fibrotic marker genes alpha smooth muscle actin (α-SMA) and connective tissue growth factor (CTGF) in the skin lesions. The SC injection of ANE successfully induced skin fibrosis, exhibiting characteristics similar to those of OSF. This model may facilitate future studies of the mechanism underlying OSF.

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Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Dermal Fibrosis Model Induced by Areca Nut Extract that Mimics Oral Submucous Fibrosis

Chiang

Chen

et al. 2016

PLoS ONE

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“…Tanshinone IIA is a diterpene extracted from Salvia miltiorrhiza , a popular herb that has been safely and widely used in China and other Asian countries. Previous studies have demonstrated that Tanshinone IIA exerts prominent antifibrotic effect through modulating TGF- β signaling pathway and anti-inflammatory effect through inhibiting MCP-1 expression [ 16 – 24 ]. Thus, we hypothesized that Tanshinone IIA might attenuate renal fibrosis after AKI via regulating recruitment of fibrocytes into the kidney through its inhibition on TGF- β and MCP-1 in a mouse model of folic acid induced kidney injury.…”

Section: Introductionmentioning

confidence: 99%

Tanshinone IIA Attenuates Renal Fibrosis after Acute Kidney Injury in a Mouse Model through Inhibition of Fibrocytes Recruitment

Jiang

Shao

Jin

et al. 2015

BioMed Research International

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Acute kidney injury (AKI) is associated with an increased risk of developing advanced chronic kidney disease (CKD). Yet, effective interventions to prevent this conversion are unavailable for clinical practice. In this study, we examined the beneficial effects of Tanshinone IIA on renal fibrosis in a mouse model of folic acid induced AKI. We found that Tanshinone IIA treatment significantly attenuated the folic acid elicited kidney dysfunction on days 3, 14, and 28. This effect was concomitant with a much lessened accumulation of fibronectin and collagen in tubulointerstitium 28 days after folic acid injury, denoting an ameliorated renal fibrosis. The kidney protective and antifibrotic effect of Tanshinone IIA was likely attributable to an early inhibition of renal recruitment of fibrocytes positive for both CD45 and collagen I. Mechanistically, Tanshinone IIA treatment not only markedly diminished renal expression of chemoattractants for fibrocytes such as TGFβ1 and MCP-1, but also significantly reduced circulating fibrocytes at the acute phase of kidney injury. These data suggested that Tanshinone IIA might be a novel therapy for preventing progression of CKD after AKI.

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“…The latest studies in different fields have suggested the anti-fibrotic activity of Sal B in multiple organ systems such as pulmonary fibrosis, hepatic fibrosis and oral submucous fibrosis [14–17, 26–28]. These studies revealed the ability of Sal B to prevent fibrotic changes both in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Salvianolic acid B reduced the formation of epidural fibrosis in an experimental rat model

et al. 2016

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BackgroundSalvianolic acid B (Sal B) was newly reported to be able to attenuate fibrosis in the animal model. The aim of the present study was to investigate the effect of the intragastric application of Sal B on the prevention of epidural fibrosis (EF).MethodsForty healthy adult male Wistar rats were divided into four treatment groups (n = 10 per group): (1) 10 mg/kg Sal B, (2) 30 mg/kg Sal B, (3) 50 mg/kg Sal B and (4) Saline (vehicle treatment, control group). All animals underwent a laminectomy at the lumbar 1–2 (L 1–2) level. After intragastric treatment, all rats were sacrificed at post-operative week 8. The extent of the epidural scar, the regeneration of the vasculature and the expression levels of vascular endothelial growth factor (VEGF) were analysed.ResultsThe animals’ recovery was uneventful during the experimental period. The extent of the epidural scar, the regeneration of the vasculature and the expression levels of VEGF suggested better outcomes in the Sal B-treated groups. Sal B exerted the ability to prevent the formation of an epidural scar and vascularization at the laminectomy sites. The effects of Sal B were dose-dependent, with the 50 mg/kg Sal B group showing the best outcomes compared with the other groups.ConclusionsPost-operative intragastric application of Sal B can prevent the formation of epidural scarring. Sal B exerted these effects in a dose-dependent manner, and 50 mg/kg dose was shown to be the best effect in the present study. The results of this study reveal that Sal B could be a potential therapy for EF and valuable for further research.

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Inhibition of Tanshinone IIA, Salvianolic Acid A and Salvianolic Acid B on Areca Nut Extract-Induced Oral Submucous Fibrosis in Vitro

Cited by 31 publications

References 33 publications

Characterization of a Novel Dermal Fibrosis Model Induced by Areca Nut Extract that Mimics Oral Submucous Fibrosis

Characterization of a Novel Dermal Fibrosis Model Induced by Areca Nut Extract that Mimics Oral Submucous Fibrosis

Tanshinone IIA Attenuates Renal Fibrosis after Acute Kidney Injury in a Mouse Model through Inhibition of Fibrocytes Recruitment

Salvianolic acid B reduced the formation of epidural fibrosis in an experimental rat model

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