Inhibition of SRC-mediated integrin signaling in bone marrow niche enhances hematopoietic stem cell function

Roy, Irene Mariam; Anu, P.V.; Zaunz, Samantha; Reddi, Srinu; Giri, Aravind M.; Sankar, Rithika Saroj; Schouteden, Sarah; Huelsken, Joerg; Verfaillie, Cathérine; Khurana, Satish

doi:10.1016/j.isci.2022.105171

Cited by 2 publications

(1 citation statement)

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“…Hematopoietic stem cells in the bone marrow clonally proliferate, leading to leukaemia, a kind of blood cancer (Akinduro et al, 2018;Roy et al, 2022;Silva et al, 2021). Leukemia new cases were reported at a rate of 14.1 per 100,000 adult adults annually.…”

Section: Introductionmentioning

confidence: 99%

Selective binding and induction of death in leukemia cells by Biodegradable magnetized iron nanodiscs

Kharat

Pottathil

2023

Preprint

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Leukemia is a blood malignancy with a rate of new cases of 14.1 per 100,000 adults per year. Leukemic cells seek a way to escape from the cytotoxic effects of chemotherapy drugs, leading to treatment failure. In this paper, we demonstrated the separation of circulating leukaemia cells using an antibody-conjugated iron oxide nanodisc (MND-Ab) under a low magnetic field gradient. Biodegradable magnetic nanodiscs were prepared by using iron oxide, BSA and the antibody IPO-M6, which is an anti-HL-60 antibody. Atomic force microscope studies show that the nano-discs were sprayed uniformly with an average size of 22+/-2.1 nm. The efficiency of cell capture was monitored with Prussian blue staining and fluorescence microscopy. A bar magnet was placed near the plate at 2 mm distance from the centre of the plate. The labelled cells were gathered near the magnet after 2 minutes. When the MND-Ab-HL60 cells were exposed to magnetic oscillations (5 min) 10 htz, 90 Guass), 92–94% of the cells were found apoptotic or dead in treated HL60 cells compared to the untreated control.

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Section: Introductionmentioning

confidence: 99%