Inhibition of sporulation in the water mold Blastocladiella emersonii by antipain

Correa, Jaime; Lemos, Elenice Moreira; Lodi, Wilson Roberto Navega

doi:10.1016/0012-1606(78)90252-x

Cited by 15 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sporulation in B. emersonii is blocked by antipain, an inhibitor of the alkaline proteinase. The effect is not reversible, but antipain is effective only during the first 60 min of sporulation, suggesting a critical role for the proteinase for a limited period of time during the initial phases of sporulation (62). Although sporulation in yeast proved insensitive to most inhibitors of proteinases tested (23), it is partially affected by mutations which result in a lack of proteinase A (200) and proteinase B (358).…”

Section: (134) Activation and Inactivation Of Specific Proteinsmentioning

confidence: 99%

Comparative biochemistry of the proteinases of eucaryotic microorganisms.

North¹

1982

Microbiological Reviews

196

View full text Add to dashboard Cite

Section: (134) Activation and Inactivation Of Specific Proteinsmentioning

confidence: 99%

Comparative biochemistry of the proteinases of eucaryotic microorganisms.

North¹

1982

Microbiological Reviews

196

View full text Add to dashboard Cite

“…Our previous work showed that the membrane-bound glycoproteins of this mould in the vegetative growing phase mostly disappears with induction of spherulation (12,17). The importance of some proteinases for the progress of sporulation has also been reported in other microorganisms (6,34). In the present study, however, we have observed that the proteinase activity in the membrane fraction decreases gradually during the morphological changes.…”

Section: Discussionmentioning

confidence: 64%

A trypsin-like serine proteinase from plasmodial membrane of Physarum polycephalum.

Morita

Kan

Sugano

et al. 1996

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

A trypsin-like serine proteinase was found in a plasma membrane fraction from the plasmodia of Physarum polycephalum. It was solubilized from the plasma membrane fraction with 1% (w/v) Triton X-100 and purified by a series of column chromatographic steps followed by polyacrylamide gel electrophoresis (PAGE). The molecular mass of the enzyme was estimated to be 130 kDa by SDS-PAGE under non-reducing conditions. This enzyme exhibited maximal activity at pH 9 and at 35°C with the substrate Boc-Phe-Ser-Arg-MCA, and was sensitive to various serine proteinase inhibitors and trypsin inhibitors. The enzyme was significantly labeled with [3H]diisopropylfluorophosphate (DFP); however, a clear decrease in the amount of the enzyme bound-[3H]DFP was observed in the presence of p-nitrophenyl p'-guanidinobenzoate (NPGB), an active site titrant for trypsin. The activity was completely inhibited in the presence of EDTA or EGTA, but the metal-depleted enzyme was reactivated by the addition of Cart Moreover, this enzyme was capable of cleaving several synthetic peptides at the carboxyl side of arginine, but did not work on synthetic substrates for chymotrypsin, elastase and aminopeptidase. The enzyme showed an affinity to Con A, indicating that it contains a carbohydrate moiety. Inhibition of the activity by a serine proteinase inhibitor resulted in cessation of the DNA synthesis in growing plasmodia without loss of the protein synthesis. Thus, this trypsin-like serine proteinase may have a regulatory role in the growth and development of the slime mould.

show abstract

“…Vegetative cells were obtained and assayed by the procedure described previously [12], with small modifications. Briefly, 0.3×10 5 zoospores were inoculated into 6 cm diameter Petri dishes containing 3 ml of growth medium DM 2 with or without 10 −3 M CaCl 2 and incubated for 12 h at 14.5±1°C, where vegetative cells grew adhered to the bottom of the dishes.…”

Section: Methodsmentioning

confidence: 99%

The induction of sporulation in the aquatic fungusBlastocladiella emersoniiis dependent on extracellular calcium

Coutinho

Corrêa

1999

FEMS Microbiology Letters

View full text Add to dashboard Cite

Induction of sporulation in Blastocladiella emersonii is absolutely dependent on extracellular calcium. Vegetative cells grown in media with or without calcium do not sporulate in media devoid of calcium or in CaCl(2) with EGTA. Calcium channel blockers, CoCl(2) and nifedipine, and ionophore A23187 inhibited the induction of sporulation. The calmodulin antagonists trifluoperazine and chlorpromazine inhibited the sporulation when present in the cultures at least 60 min after induction. So, calcium that is accumulated during growth is not sufficient or is not mobilized to initiate sporulation, and a calcium influx is likely to occur by type II calcium channel functions, essential for the response to nutritional starvation. A calmodulin-like protein has been suggested to mediate calcium events in sporulation.

show abstract

Inhibition of sporulation in the water mold Blastocladiella emersonii by antipain

Cited by 15 publications

References 25 publications

Comparative biochemistry of the proteinases of eucaryotic microorganisms.

Comparative biochemistry of the proteinases of eucaryotic microorganisms.

A trypsin-like serine proteinase from plasmodial membrane of Physarum polycephalum.

The induction of sporulation in the aquatic fungusBlastocladiella emersoniiis dependent on extracellular calcium

Contact Info

Product

Resources

About