Inhibition of skin pigmentation by an extract of <i>Lepidium apetalum</i> and its possible implication in IL‐6 mediated signaling

Choi, Hyunjung; Ahn, Soo Whan; Lee, Byeong G.; Chang, Ih-Seop; Hwang, Jae Sung

doi:10.1111/j.1600-0749.2005.00266.x

Cited by 55 publications

(44 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…25,26) A plethora of growth factor signaling pathways is implicated in melanocyte biology, and some of them have been shown to be relevant to MITF. 17,25,27) Therefore, the expression level of MITF was examined with ADSC-CM incubation in this study, but the expression level was not changed. TGF-b1 reportedly down-regulates MITF and induces a significant delay in extracellular signal related kinase (ERK) activation that also contributes to depigmentation.…”

Section: Discussionmentioning

confidence: 99%

Whitening Effect of Adipose-Derived Stem Cells: A Critical Role of TGF-.BETA.1

Kim

Park²,

Ahn³

et al. 2008

Biological & Pharmaceutical Bulletin

101

View full text Add to dashboard Cite

It has been demonstrated that adipose-derived stem cells (ADSCs) secrete cytokines and exhibit diverse pharmacological actions. The present study examined the unknown pharmacological action of ADSCs regarding whitening effects. A conditioned medium of ADSCs (ADSC-CM) was harvested and the whitening effect of ADSC-CM was studied in melanoma B16 cells. ADSC-CM treatment inhibited the synthesis of melanin and the activity of tyrosinase in a dose dependent manner. To clarify the underlying mechanisms of the whitening action of ADSCs, protein levels of melanogenic proteins were measured by Western blot. Although expressions of microphthalmia-associated transcription factor and tyrosinase-related protein 2 (TRP2) remained unchanged, those of tyrosinase and TRP1 were down-regulated. Transforming growth factor-b b1 (TGF-b b1), a potent regulator of melanogenic proteins, was neutralized by the addition of a blocking antibody to ADSC-CM, and down-regulated expression of tyrosinase and TRP1 was almost reversed. Collectively, these results indicate that secretary factors of ADSC inhibit melanin synthesis by down-regulating the expression of tyrosinase and TRP1, which are mainly mediated by TGF-b b1.Key words adipose-derived stem cell; melanin; tyrosinase; tyrosinase-related protein 1 (TRP1); transforming growth factor-b1 (TGF-b1)

show abstract

Section: Discussionmentioning

confidence: 99%

Whitening Effect of Adipose-Derived Stem Cells: A Critical Role of TGF-.BETA.1

Kim

Park²,

Ahn³

et al. 2008

Biological & Pharmaceutical Bulletin

101

View full text Add to dashboard Cite

show abstract

“…Furthermore, C. spinosa extract was reported to be rich in flavonoids such as kaempferol, rutin, quercetin, and quercetin derivatives [3,5], which are known to have antiallergic, anti-inflammatory, and antioxidant [6,7] properties. Recently, the effect of plant extracts on melanogenesis has been reported [8][9][10] and compounds, such as glycyrrhizin, quercetin, and scoparone, were found to stimulate melanogenesis [11][12][13]. Melanin, the product of melanogenesis, plays a major role in photoprotection.…”

Section: Introductionmentioning

confidence: 98%

Effect of Tunisian Capparis spinosa L. extract on melanogenesis in B16 murine melanoma cells

et al. 2009

View full text Add to dashboard Cite

The effect of Tunisian Capparis spinosa L. aromatic plant extract on melanogenesis regulation in B16 murine melanoma cells was investigated. B16 cells were treated with 0.0005, 0.005, and 0.05% (w/v) C. spinosa extract after which the melanin content and cell viability were measured. To clarify the mechanism behind melanogenesis regulation, the expression of tyrosinase was determined. Results showed that the extract had a significant stimulative effect on melanogenesis in B16 cells in a dose-dependent manner without cytotoxicity. Western blot analysis showed that expression of tyrosinase in cells treated with 0.03% (w/v) C. spinosa extract increased by 12.5- and 20-fold after 24 and 48 h of incubation, respectively, compared with untreated cells. HPLC analysis of the extract revealed the presence of 1% quercetin, a known melanogenesis stimulator, indicating that our findings may be attributed to quercetin; however, other compounds present in the extract may also have an effect on the overall ability of the extract to stimulate melanogenesis. We report here that Tunisian C. spinosa leaf extract can stimulate melanogenesis in a dose-dependent manner without cytotoxicity by increasing tyrosinase protein expression and has the potential to be used as a possible tanning agent or as a treatment for hair depigmentation.

show abstract

“…Moreover, they found out that decrease in tyrosinase protein expression was due to decrease in mRNA levels of MITF, tyrosinase, and TRP-2. 22,38 Therefore, it is reasonable to conclude that IL-6 secreted in the CM-hCBSC played a pivotal role in the anti-melanogenesis action in this study.…”

Section: Discussionmentioning

confidence: 90%

Effect of cultured medium of human umbilical cord blood-derived mesenchymal stem cells on melanogenic enzyme activity in mouse B16 melanoma cells

Hwang

Son

Park

et al. 2014

Tissue Eng Regen Med

View full text Add to dashboard Cite

The cultured medium of mesenchymal stem cell is a valuable resource for a variety of stem cell-derived cytokines and growth factors with a potential therapeutic effect on skin diseases. Here, we investigated the effect of the cultured medium of human umbilical cord blood-derived mesenchymal stem cell (CM-hCBSC) on melanogenesis in mouse B16 melanoma cells stimulated by α-melanocyte stimulating hormone (α-MSH). Our results show that CM-hCBSC inhibits melanin synthesis due to the decrease in cellular tyrosinase activity in B16 melanoma cells without cytotoxicity. The activity of melanogenic enzymes such as tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2 were evaluated by western blot and semi-quantitative RT-PCR. The CM-hCBSC down-regulated the protein and mRNA expression levels of tyrosinase and TRP-1 in a dose-dependent manner. In addition, TRP-2 protein and mRNA expression levels were found to be down-regulated to a lesser extent. We also measured the level of cytokines in CM-hCBSC using the Luminex total system. It was found that the top enriched cytokine in the CMhCBSC was interukine-6 (IL-6), known as a potent regulator of melanin synthesis together with IL-1α and tumor necrosis factor α (TNF-α). These results indicate that secretary factors including IL-6 in the CM-hCBSC may inhibit melanin synthesis by down-regulating the expression of tyrosinase and TRP1. This study suggests that CMhCBSC has a therapeutic effect as an anti-melanogenic agent and may be effective against hyperpigmentation disorders.

show abstract

Inhibition of skin pigmentation by an extract of Lepidium apetalum and its possible implication in IL‐6 mediated signaling

Cited by 55 publications

References 35 publications

Whitening Effect of Adipose-Derived Stem Cells: A Critical Role of TGF-.BETA.1

Whitening Effect of Adipose-Derived Stem Cells: A Critical Role of TGF-.BETA.1

Effect of Tunisian Capparis spinosa L. extract on melanogenesis in B16 murine melanoma cells

Effect of cultured medium of human umbilical cord blood-derived mesenchymal stem cells on melanogenic enzyme activity in mouse B16 melanoma cells

Contact Info

Product

Resources

About