Inhibition of sirtuin 1 deacetylase by miR-211-5p provides a mechanism for the induction of cell death in breast cancer cells

Yarahmadi, Sahar; Abdolvahabi, Zohreh; Hesari, Zahra; Tavakoli-Yaraki, Masoumeh; Yousefi, Zeynab; Seiri, Parvaneh; Hosseinkhani, Saman; Nourbakhsh, Mitra

doi:10.1016/j.gene.2019.06.029

Cited by 29 publications

(19 citation statements)

References 42 publications

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“…Previous studies have demonstrated that miR‐211‐5p plays a crucial role in the onset and progression of carcinogenesis, 31,33 although the role of miR‐211‐5p and its underlying molecular mechanism have not been determined in BCa. In the present study, miR‐211‐5p was down‐regulated in BCa tumor tissues and the cell lines, whereas overexpression of miR‐211‐5p served as an anti‐proliferative and pro‐apoptotic agent in 5637 and T24 cells.…”

Section: Discussionmentioning

confidence: 99%

miR‐211‐5p is down‐regulated and a prognostic marker in bladder cancer

Wang

Liu

Zhang

et al. 2020

The Journal of Gene Medicine

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Background: The micro RNA (miRNA)/histone deacetylase 9 (HDAC9) signaling axis has been reported to be involved in initiating and developing multiple malignant tumors. In the present study, we aimed to determine whether miR-211-5p serves as a post-transcriptional regulator in bladder cancer (BCa) cell proliferation and apoptosis by targeting HDAC9. Methods: miRNA expression profiling of BCa tissues and para-carcinoma tissues was screened by miRNA microarray. After transfection with miR-211-5p mimics or short hairpin RNA of HDAC9 (sh-HDAC9), mRNA and protein expression was evaluated using a quantitative reverse transcription-polymerase chain reaction and western blotting, respectively. A bioinformatics algorithm was used, and a dual-luciferase reporter assay was performed to validate HDAC9 as a direct target of miR-211-5p. Cell proliferation was analyzed by the 3-(4, 5-dimethylthiazl2-yl)-2,5diphenyltetazolium bromide (MTT) assay. Terminal deoxynucleotidyl transferasemediated dUTP nick-end labeling (TUNEL) detection was used to evaluate apoptosis in 5637 and T24 cells. A transwell assay was used to assess migration and invasion. Results: miR-211-5p is down-regulated in BCa tumor tissues and cell lines. miR-211-5p is identified as an independent biomarker for predicting overall survival. HDAC9 is a direct target of miR-211-5p, and overexpression of miR-211-5p represses HDAC9 protein expression in vitro. Overexpression of miR-211-5p or HDAC9 knockdown significantly inhibits proliferation, migration and invasion of 5637 and T24 cells, and also induces cell apoptosis. Conclusions: miR-211-5p may play a role as a tumor suppressor and as a favourable prognostic marker in BCa.

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Section: Discussionmentioning

confidence: 99%

miR‐211‐5p is down‐regulated and a prognostic marker in bladder cancer

Wang

Liu

Zhang

et al. 2020

The Journal of Gene Medicine

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“…33 On the contrary, SIRT1 was proved to promote tumor development in breast cancer and endometrial cancer. 34,35 Alves-Fernandes et al suggested that the contradictory role of SIRT1 might due to the different cell type and SIRT1 localization. 36 A previous study revealed that SIRT1 promoted HBV replication in hepatocytes.…”

Section: Discussionmentioning

confidence: 99%

Retracted Article: MicroRNA-1271 modulates hepatitis B virus replication, cell proliferation and apoptosis in hepatitis B virus-related hepatocellular carcinoma by targeting SIRT1

Tang

Wang

et al. 2019

RSC Adv.

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“…In parallel with this aim, growing number of antimetabolites and sirtuin inhibitors are administered. As an example for the latter, inhibition of SIRT1 by the up-regulation of miR-211-5p was associated with the induction of apoptosis in breast cancer cells (91). Inhibition of SIRT2 has been confirmed to induce the susceptibility of melanoma cell to the multikinase inhibitor dasatinib (92).…”

Section: Special Aspects Of Novel Epigenetic and Metabolic Therapeutimentioning

confidence: 96%

Impact of Sirtuin Enzymes on the Altered Metabolic Phenotype of Malignantly Transformed Cells

Gaál

Csernoch

2020

Front. Oncol.

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Sirtuins compose a unique collection of histone deacetylase enzymes that have a wide variety of enzymatic activities and regulate diverse cell functions such as cellular metabolism, longevity and energy homeostasis, mitochondrial function, and biogenesis. Impaired sirtuin functions or alterations of their expression levels may result in several pathological conditions and contribute to the altered metabolic phenotype of malignantly transformed cells in a significant manner. In the twenty-first century, principles of personalized anticancer treatment need to involve not only the evaluation of changes of the genetic material, but also the mapping of epigenetic and metabolic alterations, to both of which the contribution of sirtuin enzymes is fundamental. Since sirtuins are central players in the maintenance of cellular energy and metabolic homeostasis, they are key elements in the development of metabolic transformation of cancer cells referred to as the Warburg effect. Although its most well-known features are enhanced glycolysis and excessive lactate production, Warburg effect has several aspects involving both carbohydrate, lipid, and amino acid metabolism, among which different tumor types have different preferences. Therefore, energy supply of cancer cells can be impaired by a growing number of antimetabolite agents, for which appropriate vectors are strongly needed. However, data are controversial about their tumor suppressor or oncogenic properties, the biological effects of sirtuin enzymes strongly depend on the tissue microenvironment (TME) in which they are expressed. Immune cells are regarded as key players of TME. Sirtuins regulate the survival, activation, metabolism, and mitochondrial function of these cells, therefore, they are not only single elements, but key regulators of the network that determines anticancer immunity. Altered metabolism of tumor cells induces changes in the gene expression pattern of cells in TME, due to altered concentrations of metabolite cofactors of epigenetic modifiers including sirtuins. In summary, epigenetic and metabolic alterations in malignant diseases are influenced by sirtuins in a significant manner, and should be treated in a personalized approach. Since they often develop in early stages of cancer, broad examination of these alterations is required at time of the diagnosis in order to provide a personalized combination of distinct therapeutic agents.

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Inhibition of sirtuin 1 deacetylase by miR-211-5p provides a mechanism for the induction of cell death in breast cancer cells

Cited by 29 publications

References 42 publications

miR‐211‐5p is down‐regulated and a prognostic marker in bladder cancer

miR‐211‐5p is down‐regulated and a prognostic marker in bladder cancer

Retracted Article: MicroRNA-1271 modulates hepatitis B virus replication, cell proliferation and apoptosis in hepatitis B virus-related hepatocellular carcinoma by targeting SIRT1

Impact of Sirtuin Enzymes on the Altered Metabolic Phenotype of Malignantly Transformed Cells

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