Inhibition of Simian Virus 40 Large Tumor Antigen Expression in Human Fetal Glial Cells by an Antisense Oligodeoxynucleotide Delivered by the JC Virus-Like Particle

Wang, Meilin; Tsou, Tsung-Hsuan; Chen, Lisheng; Ou, Wei-Chih; Chen, Pei-Lain; Chang, Chien‐Wen; Fung, Chiung-Yau; Chang, Deching

doi:10.1089/hum.2004.15.1077

Cited by 25 publications

(17 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This contrasts with the plasmid DNA packaged by osmotic shock, which is not well protected. 15,28 The VLP is able to accommodate and protect DNA molecules up to 2 kbp by the in vitro package method. 28 Furthermore, the efficiency of gene transduction was about 1-2% when the osmotic shock VLPs were used.…”

Section: Discussionmentioning

confidence: 99%

“…15,28 The VLP is able to accommodate and protect DNA molecules up to 2 kbp by the in vitro package method. 28 Furthermore, the efficiency of gene transduction was about 1-2% when the osmotic shock VLPs were used. 21 This is very much lower than the efficiency of gene transduction in the present system in which the efficiency was about 80% when VLPs were produced by in vivo DNA packaging.…”

Section: Discussionmentioning

confidence: 99%

“…For example, when in vitro DNA packaging using osmotic shock was carried out, 18,21 DNA molecules were diffused into the virus particles by increasing the permeability of the VLP; however, only partial or short DNA fragments could be accommodated and protected within the particle. 28 Another DNA packaging method based on capsomere reassembly has been shown to have low efficiency in terms of gene transduction because capsomere aggregation occurs during the process of assembly. 29,30 These limitations on the use of polyomavirus vectors because of problems with packaging DNA molecules need to be addressed.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Efficient gene transfer using the human JC virus-like particle that inhibits human colon adenocarcinoma growth in a nude mouse model

Chen

Wang

et al. 2010

Gene Ther

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Efficient gene transfer using the human JC virus-like particle that inhibits human colon adenocarcinoma growth in a nude mouse model

Chen

Wang

et al. 2010

Gene Ther

View full text Add to dashboard Cite

“…SVG p12, SVG, and subclones of SVG have been used in several JCPyV studies (26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41) and in more than 30 other studies in which cells of neural origin were required. Unfortunately, the source of the SVG cells was not clearly specified for some of these studies.…”

mentioning

confidence: 99%

The Human Fetal Glial Cell Line SVG p12 Contains Infectious BK Polyomavirus

Henriksen

Tylden

Dumoulin

et al. 2014

J Virol

View full text Add to dashboard Cite

The human fetal glial cell line SVG was generated in 1985 by transfecting primary fetal brain cells with a plasmid containing an origin-defective mutant of simian virus 40 (SV40). The cells, which express SV40 large T-antigen, support the replication of human JC polyomavirus (JCPyV) and have been used for JCPyV studies but also for other studies in which cells of neural origin were desirable. We intended to use the SVG p12 cells from ATCC for antiviral drug studies with JCPyV. However, during initial experiments, immunofluorescence microscopy controls unexpectedly revealed cells expressing the late viral proteins VP1, VP2/ VP3, and agno. This was confirmed by Western blotting. Since our agnoprotein antiserum is specific for BKPyV agnoprotein, infection with BKPyV was suspected. Indeed, specific BKPyV PCR of SVG p12 supernatants revealed a viral load of >1 ؋ 10

show abstract

“…One study clearly showed that JCV VLPs, consisting of Vp1 alone, were unable to protect genome-size DNA from DNase degradation (28). It has also been shown that under physiological conditions, Vp2 and VP3 are both required for SV40 Vp1 to form VLPs (12,13).…”

mentioning

confidence: 99%

JC Virus Minor Capsid Proteins Vp2 and Vp3 Are Essential for Virus Propagation

Gasparovic

Gee

Atwood

2006

J Virol

View full text Add to dashboard Cite

Virus-encoded capsid proteins play a major role in the life cycles of all viruses. The JC virus capsid is composed of 72 pentamers of the major capsid protein Vp1, with one of the minor coat proteins Vp2 or Vp3 in the center of each pentamer. Vp3 is identical to two-thirds of Vp2, and these proteins share a DNA binding domain, a nuclear localization signal, and a Vp1-interacting domain. We demonstrate here that both the minor proteins and the myristylation site on Vp2 are essential for the viral life cycle, including the proper packaging of its genome.

show abstract

Inhibition of Simian Virus 40 Large Tumor Antigen Expression in Human Fetal Glial Cells by an Antisense Oligodeoxynucleotide Delivered by the JC Virus-Like Particle

Cited by 25 publications

References 44 publications

Efficient gene transfer using the human JC virus-like particle that inhibits human colon adenocarcinoma growth in a nude mouse model

Efficient gene transfer using the human JC virus-like particle that inhibits human colon adenocarcinoma growth in a nude mouse model

The Human Fetal Glial Cell Line SVG p12 Contains Infectious BK Polyomavirus

JC Virus Minor Capsid Proteins Vp2 and Vp3 Are Essential for Virus Propagation

Contact Info

Product

Resources

About