Inhibition of self pollen germination in Senecio vulgaris L.

Warren, John; Crawford, T. J.; Oxford, Geoff

doi:10.1038/hdy.1988.6

Cited by 12 publications

(3 citation statements)

References 17 publications

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“…Self-pollinations and incompatiblepollinations result in low pollen loads due to the displacement of pollen grains, that are not firmly adhered to the stigma surface, from the stigma during fixation and staining. Similar results have been found in other Compositae (Warren et al 1988). Diflerences in adhesion of self-pollen or cross-pollen to the stigma may occur within 10-15 min of poUination in Brassica oleracea, where self-pollen is easier to remove than crosspollen (Stead et al 1979).…”

Section: Discussionsupporting

confidence: 93%

The incompatibility system in Italian red chicory (Cichorium intybus L.)

et al. 1995

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As a result of an incompatibility mechanism which prevents both selffertilization and intermating between plants with identical genotypes, inbreeding does not occur in Cichorium intybus. This self-incompatibihty system complicates the production of inbred lines and the generation of Fj hybrids in Italian red chicory.Histological observations on pollinated stigmas show that the incompatibility reaction in chicory is very rapid and either prevents pollen adhering to the stigmatic papillae or determines the arrest of pollentube growth on the stigmatic surface; incompatible pollen cannot reach the transmitting tissue of the style.Both histological and genetic analyses of F, progeny from a reciprocal cross between two unrelated plants suggest the occurrence of a sporophytic incompatibility system in Italian red chicory.Key words: Cichorium intyhus -incompatibihty -pollenstigma-interaction -diallel analysis Self-incompatibihty (SI) has been defined as a cell-cell recognition system that enables the pistil to discriminate self-pollen from non-self-pollen (Dzelzkalns et al. 1992). In the gametophytic SI system, the phenotype of pollen is determined by its own haploid S genotype. Sporophytic SI, where the incompatibility phenotype is determined by the genotype of the pollen-producing plant, has been well studied in the genus Brassica, but this SI system also occurs in species of the Compositae family (Nasrallah and Nasrallah 1993), among others.In Cichorium intybus (L.) inbreeding is hampered by an incompatibility mechanism that prevents both self-fertilization and intermating between plants with identical incompatibihty phenotypes. Some authors have suggested the presence of a sporophytic SI in the species (Pecaut 1958, 1962, Eenink 1981, but others have found indications of a gametophytic system (Bannerot and Fouilloux 1970). No information is available about SI in Italian red chicory, but it is known that SI hampers the production of inbred lines in these types of chicory. On the other hand, SI could be used in the production of hybrid seed if highly self-incompatible parents were available. Further information on the genetics of SI is thus needed for its exploitation in breeding programmes.In this paper, histological and genetic observations on an SI system in red Italian chicory type 'Rosso di Chioggia' are presented. Materials and MethodsHistological analysis: 400 C. intybus plants taken from a 'Rosso di Chioggia' population were vernalized for 15 days at 4 C and grown in the field during summer at the University of Padova's experimental farm (Italy).At fiowering, a number of randomly chosen plants were put under a cage to prevent insect pollination. On each plant, selfing and one or more cross combinations were made by hand pollination. A total of 65 mating combinations, including selfings, were evaluated on at least five fiower heads for each self-and cross-pollination.The pollinations were carried out in the early morning, covering the receptive surface of each stigma with an excess of pollen. Before pollination,...

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Section: Discussionsupporting

confidence: 93%

The incompatibility system in Italian red chicory (Cichorium intybus L.)

et al. 1995

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“…2). Similar results have been reported for other Compositae species (Gerstel and Riner 1950;Hughes and Babcock 1950;Warren et al 1988), and pollen load was used as the main parameter for assessment of SI among Crepisfoetida (Hughes and Babcock/950) and Senecio vulgaris (Warren et al 1988). In Z. angustifolia, incompatible crosses result in low pollen loads due to the dislodgement of pollen grains from the stigma during fixation and staining.…”

Section: Visible Light Microscopysupporting

confidence: 78%

“…The use of cytoplasmic stains and visible light microscopy allow a more rapid analysis of SI than embryo or seed counts, and these methods have already been used for assessing SI systems among numerous Compositae species (Crowe 1954;Hughes and Babcock 1950;Gerstel 1950;Eenink 1981a;Warren et al 1988). However, the floral morphology of some species may preclude the use of cytoplasmic stains (and microscopal methods in general) due to technical difficulties with the emasculation process required for controlled pollinations.…”

Section: Visible Light Microscopymentioning

confidence: 99%

Self-incompatibility of Zinnia angustifolia HBK (compositae)

Samaha

Boyle

Mulcahy

1989

Sexual Plant Reprod

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Visible light and UV epifluorescence microscopy were used to assess self-incompatibility (SI) in Zinnia angustifolia clones. Pistils were fixed 24 h after pollination and stained either with aniline blue in lactophenol (visible light microscopy) or decolorized aniline blue (fluorescence microscopy). Percentage of florets with embryos 21 days following pollination (% embryo set) was used as a control. Embryo set following self-or incompatible cross-pollinations ranged from 0~ to 9.9%, whereas compatible crosses yielded 55.5%-87.1% embryo set. Observations using visible light microscopy indicated pollen load and number of germinated grains were significantly higher for compatible compared to incompatible crosses, and both variables were positively correlated (r= 0189-0.96) to 0/0 embryo set. Examinations with UV epifluorescence microscopy revealed pollen load was higher and little or no callose accumulated in stigmatic papillae following compatible crosses, whereas for incompatible crosses, pollen load was low and callose lenticules were deposited in stigmatic papillae; the correlation between pollen load and 0/0 embryo set was 0.89. The intensity of callose fluorescence of the pollen tube-papillae attachment sites was quantitatively measured via microspectrofluorometry. Callose fluorescence intensity ranged from 47.9% to 62.6% for incompatible and from 6.4% to 9.9~ for compatible crosses, and was negatively correlated (r= -0.95) with 0/0 embryo set. Microscopal techniques permit rapid assessment of SI and may be used routinely when each observed or measured parameter is highly correlated to the incompatibility response.

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The Radiate Capitulum Morph of Senecio Vulgaris L. Within Sussex: The Use of GIS in Establishing Origins

Waite

Burnside

2001

GIS and Remote Sensing Applications in Biogeography and Ecology

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Inhibition of self pollen germination in Senecio vulgaris L.

Cited by 12 publications

References 17 publications

The incompatibility system in Italian red chicory (Cichorium intybus L.)

The incompatibility system in Italian red chicory (Cichorium intybus L.)

Self-incompatibility of Zinnia angustifolia HBK (compositae)

The Radiate Capitulum Morph of Senecio Vulgaris L. Within Sussex: The Use of GIS in Establishing Origins

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