Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells

Mikami, Taisei; Yoshida, Keiichiro; Sawada, Hajime; Esaki, Michiyo; Yasumura, Keisuke; Ono, Michio

doi:10.1186/s40659-015-0039-2

Cited by 18 publications

(12 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The inhibition of Rho-associated coiled coil-containing protein kinases (ROCKs) activity was shown to prevent some types of individual cell migration, as of Amoeba proteus (Kłopocka and Redowicz 2004) or breast cancer cells (Guerra et al 2017) and also some types of collective cell movement (Mikami et al 2015). However, it was also reported that ROCK inhibitors enhance other migratory activities, such as the movement of various cell types: endothelial cells from spheroids (Breyer et al 2012), human proximal tubular epithelial cells, and epithelial cells of intestinal origin (Hopkins et al 2007).…”

Section: Discussionmentioning

confidence: 99%

The migration and fusion events related to ROCK activity strongly influence the morphology of chicken embryo intestinal organoids

2018

View full text Add to dashboard Cite

The method of organoid culture has become a tool widely used in gastrointestinal research, but so far, the migration of organoids derived from gut epithelium and formed in 3D Matrigel matrix has not been reported and studied. The intestinal epithelial tissue derived from 19-day-old chicken embryo was cultured in Matrigel and the dynamic properties of the forming organoids were analyzed by time-lapse image analysis. It was observed that about one in ten organoids actively moved through the matrix, at a speed of 10–20 μm/h. Moreover, rotation was observed in the majority of organoids that did not migrate long distances. The fusion events took place between organoids, which collided during the movement or growth. In our previous paper, we showed that the presence of Toll-like receptor 4 ligand, Escherichia coli lipopolysaccharide (LPS, 1 μg/ml), increased the mean organoid diameter. Here, we confirm this result and demonstrate that the Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM) did not completely abolish organoid migration, but prevented the fusion events, in both LPS-treated and untreated cultures. In consequence, in the presence of Y-27632, the differences between cultures incubated with and without LPS were not visible. We conclude that migration and fusion of organoids may influence their morphology and suggest that these phenomena should be taken into account during the design of experimental settings. Electronic supplementary material The online version of this article (10.1007/s00709-018-1312-3) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

The migration and fusion events related to ROCK activity strongly influence the morphology of chicken embryo intestinal organoids

2018

View full text Add to dashboard Cite

show abstract

“…Thus, another hypothesis is that SdrD-Dsg1 interaction influences the ability of keratinocytes to effectively differentiate into respective cellular structures within the epidermal-stratified structure. Dsg1 also influences the Rho signalling pathway 48 , an essential pathway affecting cytoskeleton and overall migration of cells 49 . This scenario could possibly influence the ability of S. aureus to invade deep tissues and subsequently enter into the bloodstream.…”

Section: Discussionmentioning

confidence: 99%

The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells

Askarian

Ajayi

Hanssen

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Staphylococcus aureus is known as a frequent colonizer of the skin and mucosa. Among bacterial factors involved in colonization are adhesins such as the microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). Serine aspartate repeat containing protein D (SdrD) is involved in adhesion to human squamous cells isolated from the nose. Here, we identify Desmoglein 1 (Dsg1) as a novel interaction partner for SdrD. Genetic deletion of sdrD in S. aureus NCTC8325-4 through allelic replacement resulted in decreased bacterial adherence to Dsg1- expressing HaCaT cells in vitro. Complementary gain-of-function was demonstrated by heterologous expression of SdrD in Lactococcus lactis, which increased adherence to HaCaT cells. Also ectopic expression of Dsg1 in HEK293 cells resulted in increased adherence of S. aureus NCTC8325-4 in vitro. Increased adherence of NCTC8325-4, compared to NCTC8325-4ΔsdrD, to the recombinant immobilized Dsg1 demonstrated direct interaction between SdrD and Dsg1. Specificity of SdrD interaction with Dsg1 was further verified using flow cytometry and confirmed binding of recombinant SdrD to HaCaT cells expressing Dsg1 on their surface. These data demonstrate that Dsg1 is a host ligand for SdrD.

show abstract

“…However, treatment of cells with ROCK inhibitor resulted in the inhibition of cell migration as confirmed by the scratch migration assay and time-lapse microscopy ( Arora et al,2015 ). Similarly, inhibition of ROCK has shown to disturb the collective cell migration of stratified TE-10 cells derived from a human esophageal cancer specimen ( Mikami et al,2015 ). On the contrary, ROCK inhibition in porcine aortic endothelial cells resulted in a farther collective cell migration when cells were cultured on soft substrates ( Canveret al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Involvement of cAMP in the Human Serum-Induced Migration of Adipose-Derived Stem Cells

Lee¹,

Koh²,

Kim³

et al. 2016

Dev Reprod

View full text Add to dashboard Cite

Previously we observed that human adipose-derived stem cells (hADSCs) could form aggregation during culture in the presence of human serum (HS). In the present study, we have examined if the aggregation might result from the cell migration and analyzed the difference of cell adhesivity after culture in various conditions. When cells were cultured in fetal bovine serum (FBS) alone, there was no morphological change. Similarly, cells pretreated with FBS for 1 day or cultured in a mixture of FBS and HS showed little change. In contrast, cells cultured in HS alone exhibited formation of cell-free area (spacing) and/or cell aggregation. When cells cultured in FBS or pretreated with FBS were treated with 0.06% trypsin, almost cells remained attached to the dish surfaces. In contrast, when cells cultured in HS alone were examined, most cells detached from the dish by the same treatment. Treatment of cells with forskolin, isobutylmethyl xanthine (IBMX) or LY294002 inhibited the formation of spacing whereas H89 or Y27632 showed little effect. When these cells were treated with 0.06% trypsin after culture, most cells detached from the dishes as cells cultured in HS alone did. However, cells treated with IBMX exhibited weaker adhesivity than HS alone. Based on these observations, it is suggested that HS treatment might decrease the adhesivity and induce three-dimensional migration of hADSCs, in the latter of which cAMP signaling could be involved.

show abstract

Inhibition of Rho-associated kinases disturbs the collective cell migration of stratified TE-10 cells

Cited by 18 publications

References 59 publications

The migration and fusion events related to ROCK activity strongly influence the morphology of chicken embryo intestinal organoids

The migration and fusion events related to ROCK activity strongly influence the morphology of chicken embryo intestinal organoids

The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells

Involvement of cAMP in the Human Serum-Induced Migration of Adipose-Derived Stem Cells

Contact Info

Product

Resources

About