Inhibition of Rat Muscle and Liver Phosphofructokinases by High Doses of Ethanol

Abstract

Activities of both rat muscle and liver phosphofructokinases are significantly inhibited after a single ethanol intake in the dose of 2.5 g per kg of body weight. This inhibitory effect is indirect, since ethanol in concentration (50 mM) close to that established after 2.5 g per kg of body weight intake cannot decrease their activities in vitro. Inhibition of liver phosphofructokinase activity after the 5.0 g per kg ethanol intake may be direct, since liver phosphofructokinase activity decreases in vitro when ethanol is added to supernatants of rat liver tissue in 100 mM concentration. According to the results of molecular docking, ethanol at high concentrations can be bound by adenine-binding pocket of the allosteric ADP-binding site of liver phosphofructokinase (Asp543, Phe308, Phe538, and Phe671) and its activation by ADP can be blocked by C2H5OH molecule. Direct inhibition of muscle phosphofructokinase activity, probably due to the binding of ethanol to the similar ADP-binding site, is possible when the concentration of ethanol (500 mM) is much higher than the level which can be established in living cells. So, inhibition of muscle phosphofructokinase activity after a single 5.0 g per kg intake is indirect and probably linked with the inhibition of the enzyme by elevated citrate and phosphoenolpyruvate levels.

Role of the Department of Clinical Laboratory Diagnostics and Immunology of the Grodno State Medical University in Training Specialist in Clinical Laboratory Diagnostics

“…На протяжении четвертого курса обучения в университете (осенний и весенний семестры) студенты медико-диагностического факультета проходят обучение по одной из основных субдисциплин клинической лабораторной диагностики -клинической биохимии. Клиническая биохимия -учебная дисциплина, содержащая систематизированные научные знания об изменениях химического состава биологических жидкостей организма человека при различных заболеваниях и методах выявления этих изменений с целью диагностики, прогноза и коррекции лечения [4].…”

In Silico Study of Morphine-Like Effects of Ethanol Intake: Docking of Acetaldehyde Conjugates with Monoamines to the Mu-Opioid Receptor

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