Inhibition of quorum sensing-controlled biofilm formation in Pseudomonas aeruginosa by quorum-sensing inhibitors

Kalaiarasan, Ellappan; Kottha, Thirumalaswamy; Harish, Belgode Narasimha; Gnanasambandam, Vasuki; Sali, Veeresh Kumar; John, James

doi:10.1016/j.micpath.2017.08.017

Cited by 47 publications

(19 citation statements)

References 26 publications

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“…is is in line with the results of Kalaiarasan and coauthors reporting the ability of two anti-QS compounds to decrease bio lm production in two P. aeruginosa clinical isolates, a ecting both lasR and rhlR transcription [42].…”

Section: Discussionsupporting

confidence: 91%

Conjugation of LasR Quorum-Sensing Inhibitors with Ciprofloxacin Decreases the Antibiotic Tolerance ofP. aeruginosaClinical Strains

Bortolotti

Trapella

Bragonzi

et al. 2019

Journal of Chemistry

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Pseudomonas aeruginosa is a Gram-negative bacterium that commonly infects subjects with weakened immune system causing deadly infections above all at pulmonary level. During infection, P. aeruginosa produces a well-organized bacterial structure, called biofilm, activating the quorum-sensing (QS) signaling, a mechanism of gene regulation. In this work, we synthesized already known QS inhibitors (QSi) designed on the scaffold of the N-(3-oxododecanoyl) homoserine lactone (3O-C12-HSL) QS molecule and conjugated them with ciprofloxacin to inhibit P. aeruginosa biofilm formation and increase the antibiotic susceptibility of clinical strains. We identified, for the first time, a QSi conjugated with ciprofloxacin (ET37), that is able to reduce the formation of biofilm and the onset of tolerant clones in P. aeruginosa clinical strains. is compound could have a wide application in clinical setting. e possibility to affect biofilm formation in chronically infected patients, such as patients affected by cystic fibrosis, and to reduce the onset of ciprofloxacin resistance would improve patient healing and allow to decrease antibiotic drug dosage.

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Section: Discussionsupporting

confidence: 91%

Conjugation of LasR Quorum-Sensing Inhibitors with Ciprofloxacin Decreases the Antibiotic Tolerance ofP. aeruginosaClinical Strains

Bortolotti

Trapella

Bragonzi

et al. 2019

Journal of Chemistry

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“…The microtitre plate method was followed to semiquantify the biofilm-forming ability of bacterial isolates as mentioned in the previous assay according to the protocol described elsewhere (Kalaiarasan et al 2017). In brief, the overnight pure culture of each individual strain was inoculated in 200 ll of BHAI in microtitre plate and then incubation at 37°C for 24-36 h. After prescribed time period, plate was shaken to deattach the individual colonies out in the liquid and plate was washed thrice with PBS followed by pipetting slowly to remove the liquid without disrupting the biofilm.…”

Section: Screening Of Antibiofilm Activity Of Lectinmentioning

confidence: 99%

Isolation and characterization of lectin with antibacterial, antibiofilm and antiproliferative activities from Acinetobacter baumannii of environmental origin

Alyousef

Alqasim

Aloahd³

2018

J Appl Microbiol

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AB119 lectin from A.baumannii species was verified for its capability to control microbial growth and its biofilm formation. Results showed lectin was able to reduce growth as well biofilm formation of both Gram-positive and Gram-negative bacterial species. Lectin has a promising application as an antibiofilm agent to combat the growing number of multidrug-resistant pathogen-associated infections.

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“…The biofilm formation of these two bacteria after adding exogenous DPD was observed. Biofilm formation was determined using 12‐well microplate according to the previous study (Kalaiarasan et al., 2017). The 1% of prepared suspensions of bacteria was inoculated in broth and then incubated at 28°C for 24 hr.…”

Section: Methodsmentioning

confidence: 99%

Quorum sensing inhibitory effect of hexanal on Autoinducer‐2 (AI‐2) and corresponding impacts on biofilm formation and enzyme activity in Erwinia carotovora and Pseudomonas fluorescens isolated from vegetables

Zhang

Xie

et al. 2022

Food Processing Preservation

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Hexanal is an essential oil component which has quorum sensing (QS) inhibitor effect on vegetable spoilage bacteria such as Erwinia carotovora (E. carotovora) and Pseudomonas fluorescens (P. fluorescens). However, the QS inhibitory (QSI) effect of hexanal on Autoinducer‐2 (AI‐2), biofilm formation, and enzyme activity is not well understood. Our results showed that the contents of AI‐2 produced by E. carotovora were higher than P. fluorescens, and the 4,5‐Dihydroxy‐2,3‐pentanedione (DPD) concentrations were significantly (p < .05) inhibited by hexanal. Adding DPD exogenously could form more biofilm when inhibited by 1/2 of minimum inhibitory concentration (MIC) of hexanal, which showed that QSI effect of hexanal on biofilm was due to its inhibition on AI‐2. There was no significant increase in enzyme activity, which suggested that enzyme inhibition by hexanal may not due to AI‐2. Understanding the relationship between QSI effect of hexanal on AI‐2, biofilm formation, and enzyme activity can improve to suppress decomposition of vegetables after harvest. Practical applications Hexanal is an EO component which has shown good QSI effect on biofilm formation and exoenzyme activities of P. fluorescens and E. carotovora. However, the reason for the QSI effect of hexanal was still unclear. In this study, hexanal as a QS inhibitor on these two vegetable spoilage bacteria was studied from the perspective of AI‐2 signal molecule. Understanding the QS inhibition of hexanal on AI‐2 signal molecule of spoilage bacteria and its relationship with biofilm formation and enzyme activity was necessary for the application of more EO components as QS inhibitor to suppress corruption of vegetables after harvest.

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Inhibition of quorum sensing-controlled biofilm formation in Pseudomonas aeruginosa by quorum-sensing inhibitors

Cited by 47 publications

References 26 publications

Conjugation of LasR Quorum-Sensing Inhibitors with Ciprofloxacin Decreases the Antibiotic Tolerance ofP. aeruginosaClinical Strains

Conjugation of LasR Quorum-Sensing Inhibitors with Ciprofloxacin Decreases the Antibiotic Tolerance ofP. aeruginosaClinical Strains

Isolation and characterization of lectin with antibacterial, antibiofilm and antiproliferative activities from Acinetobacter baumannii of environmental origin

Quorum sensing inhibitory effect of hexanal on Autoinducer‐2 (AI‐2) and corresponding impacts on biofilm formation and enzyme activity in Erwinia carotovora and Pseudomonas fluorescens isolated from vegetables

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