Inhibition of Quorum Sensing and Biofilm Formation in <i>Chromobacterium violaceum</i> by Fruit Extracts of <i>Passiflora edulis</i>

Venkatramanan, Mahendrarajan; Ganesh, P. Sankar; Senthil, Renganathan; Akshay, Jeyachandran; Ravi, Arumugam Veera; Kulanthaivel, Langeswaran; Vadivelu, Jamuna; Nagarajan, S.; Kaliaperumal, Rajendran; Shankar, Esaki Muthu

doi:10.1021/acsomega.0c02483

Cited by 67 publications

(45 citation statements)

References 44 publications

(79 reference statements)

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“…It was seen that the methanolic extract of C. indica inhibited growth at 0.5mg/ml whereas a study has reported that C. violaceum inhibited growth at 5mg/ml [42]. But in our study the extract was found to show promising activity of anti QS at sub MIC level in dose dependent manner as concentration increases at 58.13% and 42.27%in presence of 0.5mg/ml when compared to control.…”

Section: Effect Of Qs Mediated Virulence Factors In P Aeruginosa By Fruit Extractcontrasting

confidence: 69%

Attenuation of Quorum Sensing Controlled Virulence Factors and Biofilm Formation by Edible Fruit Extract of Coccinia indica against Pseudomonas aeruginosa

Devi

Ganesh

Girija

et al. 2021

JPRI

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Background: Pseudomonas aeruginosa is a Gram-negative opportunistic human pathogen that mainly infects immunocompromised individuals and patients with urinary tract infection and chronic infections of the respiratory pathways, including cystic fibrosis. Many quorum sensing (QS) controlled components such as bio surfactants and swarming motilities play an important role in the establishment of biofilms. Targeting these factors through anti-QS strategies prevent biofilm formation and treating infections. Coccinia indica commonly called little gourd is used to treat diabetes, wound, burn infections and has antioxidant, antibacterial and antitussive properties. Methods: The methanolic fruit extract of C. indica was prepared and screened for anti-QS and anti-biofilm formation activity. Pyocyanin inhibition, rhamnolipid, crystal violet staining assay tests was performed and the extract was observed under fluorescent microscope. Results: The results obtained are as follows - the fruit extract inhibits the pyocyanin at 58.13% and 42.27% at 0.5 mg/ml and 1.0 mg/ml, biofilm at 69.86% and 49.06% at 0.5 mg/ml and 1.0 mg/ml, inhibits rhamnolipid assay and under fluorescent microscope it is seen scattered whereas control produce biofilm matrix like appearance. Conclusion: Since less study has been made on the quorum sensing and biofilm activity of C.indica our study aimed to fulfil it and it was found that it exhibits good biofilm formation and thus can be used for treating infections.

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Section: Effect Of Qs Mediated Virulence Factors In P Aeruginosa By Fruit Extractcontrasting

confidence: 69%

Attenuation of Quorum Sensing Controlled Virulence Factors and Biofilm Formation by Edible Fruit Extract of Coccinia indica against Pseudomonas aeruginosa

Devi

Ganesh

Girija

et al. 2021

JPRI

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“…Hydrophobic π-mediated interactions with Tyr111 and Phe126 pocket residues were significant for these synthesized ligands. Finally, the active natural metabolites isolated from Passiflora edulis ethyl acetate extract showed relevant accommodation of the CviR binding site, particularly for hexadecanoic acid methyl ester [60]. Polar interactions with Tyr84 as well as hydrophobic contacts with Ile57, Tyr80, Leu85, Tyr88, Ile99, Trp111, Phe115, Met135, and Ile153 were found consistent across the top active metabolites.…”

Section: Discussionmentioning

confidence: 85%

Computational and Biological Evaluation of β-Adrenoreceptor Blockers as Promising Bacterial Anti-Virulence Agents

et al. 2022

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Bacterial resistance to antibiotics is an increasing public health threat as it has the potential to affect people at any stage of life, as well as veterinary. Various approaches have been proposed to counteract the bacterial resistance development. Tackling bacterial virulence is one of the most promising approaches that confer several merits. The bacterial virulence is mainly regulated by a communication system known as quorum sensing (QS) system. Meanwhile, bacteria can sense the adrenergic hormones and eavesdrops on the host cells to establish their infection, adrenergic hormones were shown to enhance the bacterial virulence. In this study, β-adrenoreceptor blockers were proposed not only to stop bacterial espionage on our cells but also as inhibitors to the bacterial QS systems. In this context, a detailed in silico study has been conducted to evaluate the affinities of twenty-two β-blockers to compete on different structural QS receptors. Among the best docked and thermodynamically stable β-blockers; atenolol, esmolol, and metoprolol were subjected to further in vitro and in vivo investigation to evaluate their anti-QS activities against Chromobacterium violaceum, Pseudomonas aeruginosa and Salmonella typhimurium. The three tested β-blockers decreased the production of QS-controlled C. violaceum, and the formation of biofilm by P. aeruginosa and S. typhimurium. Additionally, the tested β-blockers down-regulated the P. aeruginosa QS-encoding genes and S. typhimurium sensor kinase encoding genes. Furthermore, metoprolol protected mice against P. aeruginosa and S. typhimurium. Conclusively, these investigated β-blockers are promising anti-virulence agents antagonizing adrenergic hormones induced virulence, preventing bacterial espionage, and blocking bacterial QS systems.

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“…Comparable residue-wise bindings were also demonstrated for several synthetic oxazoline/2-imidazoline-based derivatives through molecular docking-coupled dynamics simulations [ 60 ]. Similarly, isolates from Passiflora edulis showed the relevant accommodation of the CviR binding site which is mediated through balanced hydrophilic–hydrophobic contacts with Ile57, Tyr80, Tyr84, Leu85, Tyr88, Ile99, Trp111, Phe115, Met135, and Ile153 [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Repurposing α-Adrenoreceptor Blockers as Promising Anti-Virulence Agents in Gram-Negative Bacteria

et al. 2022

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Antimicrobial resistance is among the world’s most urgent public health problems. Diminishing of the virulence of bacteria is a promising approach to decrease the development of bacterial resistance. Quorum sensing (QS) systems orchestrate the bacterial virulence in inducer–receptors manner. Bacteria can spy on the cells of the host by sensing adrenergic hormones and other neurotransmitters, and in turn, these neurotransmitters can induce bacterial pathogenesis. In this direction, α-adrenergic blockers were proposed as an anti-virulence agents through inhibiting the bacterial espionage. The current study aimed to explore the α-blockers’ anti-QS activities. Within comprehensive in silico investigation, the binding affinities of seven α-adrenoreceptor blockers were evaluated towards structurally different QS receptors. From the best docked α-blockers into QS receptors, terazosin was nominated to be subjected for further in vivo and in vitro anti-QS and anti-virulence activities against Chromobacterium violaceum and Pseudomonas aeruginosa. Terazosin showed a significant ability to diminish the QS-controlled pigment production in C. violaceum. Moreover, Terazosin decreased the P. aeruginosa biofilm formation and down-regulated its QS-encoding genes. Terazosin protected mice from the P. aeruginosa pathogenesis. In conclusion, α-adrenergic blockers are proposed as promising anti-virulence agents as they hinder QS receptors and inhibit bacterial espionage.

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Inhibition of Quorum Sensing and Biofilm Formation in Chromobacterium violaceum by Fruit Extracts of Passiflora edulis

Cited by 67 publications

References 44 publications

Attenuation of Quorum Sensing Controlled Virulence Factors and Biofilm Formation by Edible Fruit Extract of Coccinia indica against Pseudomonas aeruginosa

Attenuation of Quorum Sensing Controlled Virulence Factors and Biofilm Formation by Edible Fruit Extract of Coccinia indica against Pseudomonas aeruginosa

Computational and Biological Evaluation of β-Adrenoreceptor Blockers as Promising Bacterial Anti-Virulence Agents

Repurposing α-Adrenoreceptor Blockers as Promising Anti-Virulence Agents in Gram-Negative Bacteria

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