1984
DOI: 10.1128/mcb.4.10.2082
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Inhibition of protein synthesis stabilizes histone mRNA.

Abstract: The inhibition of protein synthesis in exponentially growing S49 cells leads to a specific fivefold increase in histone mRNA in 30 min. The rate of transcription of histone mRNA, measured in intact or digitoninpermeabilized cells, is increased slightly, if at all, by cycloheximide inhibition of protein synthesis. Both approach-to-equilibrium labeling and pulse-chase experiments show that cycloheximide prolongs histone mRNA half-life from approximately 30 min to >2 h. Histone mRNA made before the addition of cy… Show more

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Cited by 118 publications
(89 citation statements)
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References 70 publications
(48 reference statements)
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“…These inhibitors were carefully selected because another protein synthesis inhibitor, puromycin, induces the synthesis of heat shock proteins (33), presumably due to the formation of abnormal proteins resulting from the release of the nascent peptide. The stabilization of HSP70 mRNA by cycloheximide is a feature that is shared with many growthregulated mRNAs (19,21,31,44,53,60,61).…”
Section: Discussionmentioning
confidence: 99%
“…These inhibitors were carefully selected because another protein synthesis inhibitor, puromycin, induces the synthesis of heat shock proteins (33), presumably due to the formation of abnormal proteins resulting from the release of the nascent peptide. The stabilization of HSP70 mRNA by cycloheximide is a feature that is shared with many growthregulated mRNAs (19,21,31,44,53,60,61).…”
Section: Discussionmentioning
confidence: 99%
“…Changes in the rate of transcription of specific genes or alterations in transcript stability during inhibition of protein synthesis are frequently considered evidence for labile negative or positive regulatory proteins (13,19,22,23,28,35,39,45). In the present case, a short-lived positive regulatory VOL.…”
Section: Discussionmentioning
confidence: 99%
“…If protein synthesis, then, is also inhibited in such a culture, the histone mRNA level rises, in some cases reaching levels above that found in the uninhibited control (9,22,27,31). If these larger changes in histone mRNA levels are reflected in a larger recovery of histone protein synthesis, the relationship between these two would be further substantiated.…”
mentioning
confidence: 91%