Inhibition of protein synthesis in intact HeLa cells by Shigella dysenteriae 1 toxin

Brown, J E; Rothman, Sara W.; Doctor, Bhupendra P.

doi:10.1128/iai.29.1.98-107.1980

Cited by 80 publications

(29 citation statements)

References 22 publications

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“…Morphological damage and detachment of the epithelial cells were more pronounced after 48 h than after 24 h of incubation. Comparable results were found for T. pallidum, Shigella dysenteriae, Escherichia coli, Vibrio cholerae, and Yersinia enterocolitica (4,8,10,18), indicating that T. denticola L12D and T. vincentii RitzA share potential pathogenic properties with these pathogens. However, in these investigations attachment of the microorganisms to the cultured cells was essential for the induction of morphological damage.…”

Section: Discussionmentioning

confidence: 78%

Adherence of oral treponemes and their effect on morphological damage and detachment of epithelial cells in vitro

Reijntjens¹,

Mikx²,

Wolters-Lutgerhorst³

et al. 1986

Infect Immun

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The present study shows adherence of Treponema denticola L12D and Treponema vincentii RitzA to guinea pig ear epithelial cells in vitro. The number of adhering organisms was positively related with the treponemal concentration and contact time. Incubation of washed T. denticola L12D and T. vincentii RitzA organisms or their culture supernatants with the epithelial cells induced morphological damage and detachment of these cells. In addition, indications for inhibition of epithelial cell proliferation were found. The activity of the supernatants was dose dependent, heat sensitive, and sensitive to sulfhydryl-containing components, suggesting the presence of enzymatic activity that might be of importance in the pathogenicity of these oral treponemes.

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Section: Discussionmentioning

confidence: 78%

Adherence of oral treponemes and their effect on morphological damage and detachment of epithelial cells in vitro

Reijntjens¹,

Mikx²,

Wolters-Lutgerhorst³

et al. 1986

Infect Immun

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“…(i) Secretory IgA intestinal antibodies rather than serum antibodies should be measured. (ii) The antibody response to Shiga toxin may be muted, because this toxin is such a powerful suppressant of protein synthesis (6). (iii) Because of its potency, Shiga toxin may destroy the antigen-processing cells that pinocytose it and, in this way, the immune response may be muted.…”

Section: Discussionmentioning

confidence: 99%

Antibodies to shiga holotoxin and to two synthetic peptides of the B subunit in sera of patients with Shigella dysenteriae 1 dysentery

et al. 1992

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Acute- and convalescent-phase sera from 18 Thai patients and convalescent-phase sera from two Israeli patients and one Bangladeshi patient with Shigella dysenteriae 1 (Shiga) dysentery were tested by enzyme-linked immunosorbent assay to detect antibodies that bind S. dysenteriae lipopolysaccharide (LPS), Shiga holotoxin, or two synthetic peptides representing epitopes from the B subunit of Shiga toxin. Paired sera from 24 Maryland adults with Shigella flexneri 2a or Shigella sonnei diarrhea served as negative controls. Of the 16 paired Thai serum samples tested for immunoglobulin G LPS antibody, 10 had greater than or equal to 4-fold rises (the two subjects with the highest convalescent-phase titers exhibited toxin-neutralizing activity); acute-phase specimens from four of the remaining six individuals already had elevated Shiga LPS titers in their acute specimens ranging from 1:800 to 1:12,800. Similarly, convalescent-phase sera from the two Israeli patients and the Bangladeshi patient revealed LPS titers of 1:800 to 1:3,200. In contrast, none of the Maryland volunteers with S. flexneri or S. sonnei diarrhea manifested rises in Shiga anti-LPS (P less than 0.00001 versus 10 of 16 Thai patients). Only 4 of the 18 Thai patients had significant rise in antibody to purified Shiga toxin, while one of the two Israeli patients and the one Bangladeshi patient had elevated convalescent-phase titers. None of the sera that reacted with Shiga holotoxin had antibody that bound to the peptides. This report, which describes a search for serum antibodies that bind Shiga toxin in patients with Shiga dysentery, demonstrates such antibodies in only a minority of patients with bacteriologically confirmed disease. During Shiga dysentery, Shiga toxin may be elaborated in such small quantities in vivo that it fails to elicit an immune response in most patients even though it may exert biological effects. In this behavior Shiga toxin resembles tetanus toxin, another potent exotoxin that fails to elicit antitoxic responses in people who recover from clinical tetanus.

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“…Other analyses performed on some bacterial extracts included tests for enterotoxic activity by the rabbit ileal loop assay [30] and assessments of the paralytic and lethal activity for mice [25]. In addition, the technique of Brown et al was used to quantitate the effects of certain cytotoxic extracts on protein synthesis in HeLa cells [31]. Finally, the antigenic relationships among some of the toxins contained in extracts were assessed by immunodiffusion against rabbit antiserum to Shiga toxin.…”

Section: Methodsmentioning

confidence: 99%

Production of Shigella dysenteriae Type 1-Like Cytotoxin by Escherichia coli

O'Brien¹,

LaVeck²,

Thompson³

et al. 1982

The Journal of Infectious Diseases

417

260

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Strains of Escherichia coli previously implicated or proven to be causes of diarrhea were examined for production of a toxin similar to that of Shigella dysenteriae type 1 (Shiga). Organisms grown in an iron-depleted broth were lysed by pressure disruption followed by ultracentrifugation. Saline-dialyzed extracts were tested for cytotoxic effects on HeLa cells that were neutralizable with antiserum to Shiga toxin. Among the 13 E. coli strains so analyzed, 11 made a Shiga-like cytotoxin in levels ranging from trace (two avirulent isolates) to amounts equivalent to S. dysenteriae type 1 (two noninvasive strains that did not make E. coli heat-labile or -stable enterotoxins but were isolated from infants with diarrhea). As with extracts of Shiga toxin, lysates of these E. coli strains that produced high levels of Shiga-like toxin were enterotoxic for rabbits, paralytic and lethal for mice, and inhibited protein synthesis in HeLa cells. Thus, these data suggest that Shiga-like toxin may be another heretofore undiscovered factor in the pathogenesis of diarrhea caused by some E. coli strains.

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Inhibition of protein synthesis in intact HeLa cells by Shigella dysenteriae 1 toxin

Cited by 80 publications

References 22 publications

Adherence of oral treponemes and their effect on morphological damage and detachment of epithelial cells in vitro

Adherence of oral treponemes and their effect on morphological damage and detachment of epithelial cells in vitro

Antibodies to shiga holotoxin and to two synthetic peptides of the B subunit in sera of patients with Shigella dysenteriae 1 dysentery

Production of Shigella dysenteriae Type 1-Like Cytotoxin by Escherichia coli

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