Inhibition of Protein Synthesis by Amicetin, a Nucleoside Antibiotic^*

Abstract

Amicetin is a nucleoside antibiotic in which an aminoacyl residue is attached to the amino group

Inhibition of protein synthesis by 5'-sulfamoyladenosine

“…Other metabolites, including the 22 amino acids and the cofactors listed in the Experimental Section, were also without effect. These results paralleled the response seen with certain purine and pyrimidine analogs which interfere with protein synthesis, such as puromycin and amicetin (Bloch and Coutsogeorgopoulos, 1966).…”

“…E. coli was grown in the synthetic medium described by Gray and Tatum (1944). The procedures used for determining the potency of the compounds and for carrying out the inhibition analyses have been described previously (Bloch and Coutsogeorgopoulos, 1966).…”

Antibiotic inhibitors of the bacterial ribosome.

“…The fact that sparsomycin can interfere with '4C-chloramphenicol binding to B. stearothermophilus ribosomes (Table 5) suggests that part of the antibiotic interacts with the 50S subunit rather than with associated soluble factors. The effects of amicetin in intact cells and in a cell-free system from E. coli were studied by Block and Coutsogeorgopoulos (10). Inhibition of growth of Streptococcusfaecalis was found to require only one-tenth the concentration of amicetin needed to inhibit E. coli.…”

Synthesis and biological activity of some imidazo[4,5-b]pyrazines and their ribonucleosides as purine analogs

“…synthesized model compounds 13 and 15 (Scheme II). [5][6] pyrazine (13) was prepared in 75% yield by reacting 1 with 40% aqueous MeNH2 in the presence of a catalytic amount of Cu powder under pressure at 140-150°, followed by ring closure with ethyl orthoformate (Scheme II). An analogous route was utilized for the preparation of 15 (15) starting with the intermediate 2.…”