Inhibition of prostate tumor growth by overexpression of NudC, a microtubule motor-associated protein

Lin, Sue Hwa; Nishino, Michiya; Luo, Weiping; Aumais, Jonathan P.; Galfione, Matthew; Kuang, Jian; Yu-Lee, Li-Yuan

doi:10.1038/sj.onc.1207343

Cited by 30 publications

(27 citation statements)

References 46 publications

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“…Recently, we and other groups have found that nuclear distribution gene C (NudC) plays an important role in cytokinesis and mitosis in mammalian cells [4][5][6]. We further show that depletion of NudCL, a mammalian NudC-like protein, induces multiple mitotic defects [7].…”

Section: Dear Editorsupporting

confidence: 50%

Inhibition of cytokinesis by overexpression of NudCL that is localized to the centrosome and midbody

2009

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Section: Dear Editorsupporting

confidence: 50%

Inhibition of cytokinesis by overexpression of NudCL that is localized to the centrosome and midbody

2009

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“…The role of spindle microtubules in oocyte meiosis and early embryonic mitotic cell divisions is well documented; therefore, it was not surprising to find that a-tubulin and b-tubulin are abundantly represented in the oocyte proteome. Interestingly, while the function of NUDC is not well defined, the molecule was originally identified as a microtubuleassociated protein (Lin et al 2003) that interacts with the key regulators of mitosis such as Polo kinase, and was also found to have chaperone activity in vitro (Faircloth et al 2009). Regarding Sgt1, somatic cell mutations of this gene were found to severely compromise the organization and function of the mitotic apparatus due to the formation of a highly abnormal spindle (Martins et al 2009).…”

Section: D Proteomic Screenmentioning

confidence: 99%

Use of proteomics to identify highly abundant maternal factors that drive the egg-to-embryo transition

Yurttas¹,

Morency²,

Coonrod³

2010

Reproduction

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As IVF becomes an increasingly popular method for human reproduction, it is more critical than ever to understand the unique molecular composition of the mammalian oocyte. DNA microarray studies have successfully provided valuable information regarding the identity and dynamics of factors at the transcriptional level. However, the oocyte transcribes and stores a large amount of material that plays no obvious role in oogenesis, but instead is required to regulate embryogenesis. Therefore, an accurate picture of the functional state of the oocyte requires both transcriptional profiling and proteomics. Here, we summarize our previous studies of the oocyte proteome, and present new panels of oocyte proteins that we recently identified in screens of metaphase II-arrested mouse oocytes. Importantly, our studies indicate that several abundant oocyte proteins are not, as one might predict, ubiquitous housekeeping proteins, but instead are unique to the oocyte. Furthermore, mouse studies indicate that a number of these factors arise from maternal effect genes (MEGs). One of the identified MEG proteins, peptidylarginine deiminase 6, localizes to and is required for the formation of a poorly characterized, highly abundant cytoplasmic structure: the oocyte cytoplasmic lattices. Additionally, a number of other MEG-derived abundant proteins identified in our proteomic screens have been found by others to localize to another unique oocyte feature: the subcortical maternal complex. Based on these observations, we put forth the hypothesis that the mammalian oocyte contains several unique storage structures, which we have named maternal effect structures, that facilitate the oocyte-to-embryo transition.

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“…Overexpression of NudC in LNCaP cells inhibits their anchorage-independent growth in a soft agar colony assay. Expression of NudC in DU145 or PC-3 cells inhibits tumor growth in a s.c. xenograft model, and NudC is a potential candidate for therapeutic approaches that target the function of a microtubule motors (56).…”

Section: Microtubule Motor Proteins and Spindle Checkpoint Componentsmentioning

confidence: 99%

Microtubule-Associated Proteins as Targets in Cancer Chemotherapy

Bhat

Setaluri

2007

Clinical Cancer Research

172

138

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Natural and synthetic compounds that disrupt microtubule dynamics are among the most successful and widely used cancer chemotherapeutic agents. However, lack of reliable markers that predict sensitivity of cancers to these agents and development of resistance remain vexing issues. There is accumulating evidence that a family of cellular proteins that are associated with and alter the dynamics of microtubules can determine sensitivity of cancer cells to microtubuletargeting agents and play a role in tumor cell resistance to these agents. This growing family of microtubule-associated proteins (MAP) includes products of oncogenes, tumor suppressors, and apoptosis regulators, suggesting that alteration of microtubule dynamics may be one of the critical events in tumorigenesis and tumor progression. The objective of this review is to integrate the knowledge on these seemingly unrelated proteins that share a common function and examine their relevance to microtubule-targeting therapies and highlight MAPs-tubulin-drug interactions as a novel avenue for new drug discovery. Based on the available evidence, we propose that rational microtubule-targeting cancer therapeutic approaches should ideally include proteomic profiling of tumor MAPs before administration of microtubule-stabilizing/destabilizing agents preferentially in combination with agents that modulate the expression of relevant MAPs.

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Inhibition of prostate tumor growth by overexpression of NudC, a microtubule motor-associated protein

Cited by 30 publications

References 46 publications

Inhibition of cytokinesis by overexpression of NudCL that is localized to the centrosome and midbody

Inhibition of cytokinesis by overexpression of NudCL that is localized to the centrosome and midbody

Use of proteomics to identify highly abundant maternal factors that drive the egg-to-embryo transition

Microtubule-Associated Proteins as Targets in Cancer Chemotherapy

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