Inhibition of progesterone receptor activity in recombinant yeast by soot from fossil fuel combustion emissions and air particulate materials

Wang, Jingxian; Xie, Ping; Kettrup, A.; Schramm, Karl‐Werner

doi:10.1016/j.scitotenv.2005.01.019

Cited by 35 publications

(29 citation statements)

References 50 publications

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“…Previous studies have indicated that environmental chemicals, which can inhibit PR binding, may also have an important effect on abnormalities associated with the developing reproductive system [42,43]. Anti-progesteronic activities were found in soot from fossil fuel combustion [23]. The present study demonstrated anti-progesteronic activity in the WWTP effluents.…”

Section: Anti-progesteronic Contaminantssupporting

confidence: 68%

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In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

Rao

et al. 2013

Front. Environ. Sci. Eng.

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Effluents from wastewater treatment plant (WWTP) have been reported to have a broad spectrum of endocrine disrupting compounds (EDCs). The majority of studies have focused on the occurrence of estrogenic activity, while ignoring nuclear hormone receptors (NRs) pathways. In the present study, a battery of in vitro yeast bioassays and a cell bioassay, including antagonistic and agonistic effects on estrogen receptor (ER), androgen receptor (AR), progesterone receptor (PR), estrogenrelated receptor (ERR) and aryl hydrocarbon receptor (AHR), were conducted to evaluate the removal efficiencies of EDCs by different treatment processes of a WWTP located in Beijing. Estrogenic, anti-estrogenic, antiandrogenic, anti-progesteronic, anti-ERR and the activation of AHR activities were detected in samples from all treatment processes and the receiving water. The concentration of estrogenic contaminants with estradiol (E 2 ) equivalent concentrations ranged from 0.82 Â 10

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Section: Anti-progesteronic Contaminantssupporting

confidence: 68%

“…All samples were evaluated in triplicate for each clone. The antagonistic activity of the sample was tested by co-incubation of yeast strain with nature ligand at concentration that produced a submaximal stimulatory response [23].…”

Section: Galactosidase Assay Of Er Ar Err and Prmentioning

confidence: 99%

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

Rao

et al. 2013

Front. Environ. Sci. Eng.

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“…Serial dilutions (5 μL) of test samples were combined with 995 μL of medium containing 5×10 3 yeast cells/mL resulting in a test culture in which the volume of DMSO did not exceed 1.0% of the total volume. For determination of agonistic activities, the extracts were tested in the absence of T 3 ; and of antagonistic activities in presence of 5×10 −7 mol/L of T 3 which produced a submaximal stimulatory response (Wang et al, 2005).…”

Section: β-Galactosidase Assay Of Trmentioning

confidence: 99%

In vitro thyroid disrupting effects of organic extracts from WWTPs in Beijing

Rao

et al. 2011

Journal of Environmental Sciences

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“…However, considering that less E2 can reduce the response intensity and the signal-to-noise ratio of the test, we chose the EC submax concentration of E2 to achieve a compromise effect, and this choice was applied in all the antagonistic activity tests in this study. The concentration causing the sub-maximum effect has been used by many researchers in the antagonist tests (Bovee et al, 2008;Kolle et al, 2010;Wang et al, 2005), as well as in previous studies by our lab (Li et al, 2008a;Li et al, 2010). Therefore, our selection of EC submax is consistent with these studies and conducive for comparisons.…”

Section: Discussionmentioning

confidence: 68%

Combined action of estrogen receptor agonists and antagonists in two-hybrid recombinant yeast in vitro

Yang

Rao

et al. 2015

Ecotoxicology and Environmental Safety

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a b s t r a c tEstrogen receptor (ER) antagonistic chemicals in aquatic environments are believed to influence the binding of both endogenous and exogenous estrogens to ERs in aquatic organisms. Although the combined effects of estrogenic compounds have attracted much scientific concern, little work has been done on the influence of such antiestrogens on the biological effects of estrogens. This study focused on how the presence of different amounts of antagonists affects the results of ER agonist activity tests. To achieve this, three questions were stated and answered in sequence. A two-hybrid recombinant yeast assay mediated by ER was adopted, providing a single mode of action and single target of action for this study. Mixtures created by an ER agonist and three antagonists following the fixed-ratio principle were assessed. The concentration of 17β-estradiol causing maximum induction was set as the fixed dose of estrogen in the antagonist activity test (question 1). When the two classes of chemicals coexisted, antiestrogens, which as a whole behaved according to the concentration addition model (question 2), decreased the response of estrogen and compressed the concentration-response curves along the y-axis in the agonist activity test (question 3). This may cause the estradiol equivalent to be underestimated and potentially mask the action of estrogenic effects in toxicity evaluation of environmental samples.

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Inhibition of progesterone receptor activity in recombinant yeast by soot from fossil fuel combustion emissions and air particulate materials

Cited by 35 publications

References 50 publications

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water of different treatment processes

In vitro thyroid disrupting effects of organic extracts from WWTPs in Beijing

Combined action of estrogen receptor agonists and antagonists in two-hybrid recombinant yeast in vitro

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