Inhibition of plant pathogenic fungi by endophytic Trichoderma spp. through mycoparasitism and volatile organic compounds

Rajani, P.; Rajasekaran, C.; Vasanthakumari, M. M.; Olsson, Shannon B.; Ravikanth, G.; Shaanker, R. Uma

doi:10.1016/j.micres.2020.126595

Cited by 116 publications

(81 citation statements)

References 60 publications

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“…It was isolated from the leaves of Leucas aspera (Wild.) Link (family: Lamiaceae), a widely distributed medicinal plant reported for its antifungal, antioxidant, antimicrobial and cytotoxic activities (Prajapati et al, 2010;Rajani et al, 2020). The Trichoderma strain was grown on Petri dishes with Kaefer medium (KM) or Potato-Dextrose-Agar (PDA) medium, pH 6.5, at 23 • C in the dark (Bains and Tewari, 1987;Hill and Käfer, 2001).…”

Section: Growth Of Fungi and Spore Collectionmentioning

confidence: 99%

An Endophytic Trichoderma Strain Promotes Growth of Its Hosts and Defends Against Pathogen Attack

et al. 2020

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Plants host numerous endophytic microbes which promote plant performance, in particular under stress. A new endophytic fungus was isolated from the leaves of a deciduous wood tree Leucas aspera. Morphological inspection and multilocus phylogeny identified the fungus as a new Trichoderma strain. If applied to Arabidopsis thaliana and Nicotiana attenuata, it mainly colonizes their roots and strongly promotes initial growth of the plants on soil. The fungus grows on high NaCl or mannitol concentrations, and shows predatory capability on the pathogenic fungus Alternaria brassicicola. Colonized Arabidopsis plants tolerate higher salt stress and show lower A. brassicicola spread in roots and shoots, while arbuscular mycorrhiza formation in N. attenuata is not affected by the Trichoderma strain. These beneficial features of the novel Trichoderma strain are important prerequisites for agricultural applications.

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Section: Growth Of Fungi and Spore Collectionmentioning

confidence: 99%

An Endophytic Trichoderma Strain Promotes Growth of Its Hosts and Defends Against Pathogen Attack

et al. 2020

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“…GC-Mass analysis of the endophytic fungus studied T. longibrachiatum_strain WKA55 (MZ014020.1) revealed the presence of several volatile organic compounds (VOCs) belonging to a large class of compounds comprising hydrocarbons, alcohols, ketones, aldehydes, esters, acids, and terpenes, as well as caryophyllene derivative. In this respect, epi-β-caryophyllene was detected among the bioactive VOCs, which have been reported to possess properties of antifungal, and antioxidants activity, as well as promoting seedling growth and chlorophyll content (Lee et al, 2016;Rajani et al, 2021). Trichoderma species have been reported as excellent plantgrowth-promoting fungi, which can improve plant health by creating a favorable environment and production of a large number of secondary metabolites such as harzianic acid, harzianolde, abscisic acid, auxin-related compounds indole-3-acetic acid, indol-3-acelaldehyde, and indol-3 ethanol (Zin and Badaluddin, 2020).…”

Section: Discussionmentioning

confidence: 99%

A Novel Endophytic Trichoderma longibrachiatum WKA55 With Biologically Active Metabolites for Promoting Germination and Reducing Mycotoxinogenic Fungi of Peanut

et al. 2022

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Plant residuals comprise the natural habitat of the plant pathogen; therefore, attention is currently focusing on biological-based bioprocessing of biomass residuals into benefit substances. The current study focused on the biodegradation of peanut plant residual (PNR) into citric acid (CA) through a mathematical modeling strategy. Novel endophytic Trichoderma longibrachiatum WKA55 (GenBank accession number: MZ014020.1), having lytic (cellulase, protease, and polygalacturonase) activity, and tricalcium phosphate (TCP) solubilization ability were isolated from peanut seeds and used during the fermentation process. As reported by HPLC, the maximum CA (5505.1 μg/g PNR) was obtained after 9 days in the presence of 15.49 mg TCP, and 15.68 mg glucose. GC–MS analysis showed other bioactive metabolites in the filtrate of the fermented PNR. Practically, the crude product (40%) fully inhibited (100%) the growth and spore germination of three mycotoxinogenic fungi. On peanuts, it improved the seed germination (91%), seedling features, and vigor index (70.45%) with a reduction of abnormal seedlings (9.33%). The current study presents the fundamentals for large-scale production in the industry for the sustainable development of PNR biomass as a natural source of bioactive metabolites, and safe consumption of lignocellulosic-proteinaceous biomass, as well. T. longibrachiatum WKA55 was also introduced as a novel CA producer specified on PNR. Application of the resulting metabolite is encouraged on a large scale.

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“…This suggested that antagonism between species was one of the factors responsible for maintaining the balance among IL, UFS, and PC in H. cordata rhizomes. In addition, no contact or minimal contact between the mycelium of the different fungi suggested that the inhibition of LPFs might be achieved by producing water-soluble or volatile inhibitory chemicals ( Rajani et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Latent Pathogenic Fungi in the Medicinal Plant Houttuynia cordata Thunb. Are Modulated by Secondary Metabolites and Colonizing Microbiota Originating from Soil

Luo

Yang

et al. 2021

Polish Journal of Microbiology

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Latent pathogenic fungi (LPFs) affect plant growth, but some of them may stably colonize plants. LPFs were isolated from healthy Houttuynia cordata rhizomes to reveal this mechanism and identified as Ilyonectria liriodendri, an unidentified fungal sp., and Penicillium citrinum. Sterile H. cordata seedlings were cultivated in sterile or non-sterile soils and inoculated with the LPFs, followed by the plants’ analysis. The in vitro antifungal activity of H. cordata rhizome crude extracts on LPF were determined. The effect of inoculation of sterile seedlings by LPFs on the concentrations of rhizome phenolics was evaluated. The rates of in vitro growth inhibition amongst LPFs were determined. The LPFs had a strong negative effect on H. cordata in sterile soil; microbiota in non-sterile soil eliminated such influence. There was an interactive inhibition among LPFs; the secondary metabolites also regulated their colonization in H. cordata rhizomes. LPFs changed the accumulation of phenolics in H. cordata. The results provide that colonization of LPFs in rhizomes was regulated by the colonizing microbiota of H. cordata, the secondary metabolites in the H. cordata rhizomes, and the mutual inhibition and competition between the different latent pathogens.

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Inhibition of plant pathogenic fungi by endophytic Trichoderma spp. through mycoparasitism and volatile organic compounds

Cited by 116 publications

References 60 publications

An Endophytic Trichoderma Strain Promotes Growth of Its Hosts and Defends Against Pathogen Attack

An Endophytic Trichoderma Strain Promotes Growth of Its Hosts and Defends Against Pathogen Attack

A Novel Endophytic Trichoderma longibrachiatum WKA55 With Biologically Active Metabolites for Promoting Germination and Reducing Mycotoxinogenic Fungi of Peanut

Latent Pathogenic Fungi in the Medicinal Plant Houttuynia cordata Thunb. Are Modulated by Secondary Metabolites and Colonizing Microbiota Originating from Soil

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