Inhibition of plant essential oils and their interaction in binary combinations against tyrosinase

Abstract

Background Essential oils (EOs), derived from aromatic plants, exhibit properties beneficial to health, such as anti-inflammatory, anti-oxidative, antidiabetic, and antiaging effects. However, the effect of EOs and their interaction in binary combinations against tyrosinase is not yet known. Objective To evaluate the underlying mechanisms of EOs and their interaction in binary combinations against tyrosinas. Design We explored to investigate the inhibitory effect of 65 EOs and the interaction among cinnamon, bay, and magnolia officinalis in their binary combinations against tyrosinase. In addition, the main constituents of cinnamon, bay, and magnolia officinalis were analyzed by gas chromatography–mass spectrometry (GC–MS). Results The results showed that the most potent EOs against tyrosinase were cinnamon, bay, and magnolia officinalis with IC 50 values of 25.7, 30.8, and 61.9 μg/mL, respectively. Moreover, the inhibitory mechanism and kinetics studies revealed that cinnamon and bay were reversible and competitive-type inhibitors, and magnolia officinalis was a reversible and mixed-type inhibitor. In addition, these results, assessed in mixtures of three binary combinations, indicated that the combination of cinnamon with bay at different dose and at dose ratio had a strong antagonistic effect against tyrosinase. Magnolia officinalis combined with cinnamon or bay experienced both antagonistic and synergistic effect in anti-tyrosinase activity. Conclusion It is revealed that natural EOs would be promising to be effective anti-tyrosinase agents, and binary combinations of cinnamon, bay, and magnolia officinalis might not have synergistic effects on tyrosinase under certain condition.

Synthesis, anti-browning effect and mechanism research of kojic acid-coumarin derivatives as anti-tyrosinase inhibitors

“…2 A , Lineweaver-Burk plots showed changes in V max (maximum reaction rate) and K m (Michaelis constant). These characteristics concluded that compound 6f was an uncompetitive and non-competitive inhibitor, which could bind to both free enzyme and enzyme-substrate complexes ( You et al, 2022 ). The inhibition constant K I ( Fig.…”

Naturally-Occurring Tyrosinase Inhibitors Classified by Enzyme Kinetics and Copper Chelation

“…Molecular docking simulation has shown that the inhibitors bind to catalytic hydrogen and interact with halogen ions for the inhibitors [84]. Aromatic cinnamon and bay have also been shown to be reversible and competitive-type inhibitors [85]. T1 compound and bis-4-hydroxybenzyl sulfide is a competitive-type inhibitor to tyrosinase that is comparable to kojic acid.…”

Phytochemical characterization and multifaceted bioactivity assessment of essential oil from Ptychotis verticillata Duby: Anti-diabetic, anti-tyrosinase, and anti-inflammatory activity

“…Hence, tyrosinase activity inhibition is a prevalent approach utilized in the management and prophylaxis of pigmentation disorders [ 18 ]. Certain EOs have been shown to have anti-tyrosinase action; this might be due to the scavenging free radicals or interfering with the enzyme's catalytic mechanism [ [19] , [20] , [21] ].…”