Inhibition of Phenolic Acid Metabolism Results in Precocious Cell Death and Altered Cell Morphology in Leaves of Transgenic Tobacco Plants

Tamagnone, Lodovico; Mérida, Ángel; Stacey, Nicola; Plaskitt, Kitty A.; Parr, Adrian; Chang, Chih-Hung; Lynn, D.; Dow, J. Maxwell; Roberts, Keith; Martin, Cathie

doi:10.2307/3870905

Cited by 31 publications

(37 citation statements)

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“…The over-expression of ZmMYB31 produces a reduction of growth rate and the appearance of white lesions on the mature leaves. These lesions suggest a reduction of the levels of the hydroxycinnamic acids derivatives (Jin et al 2000;Tamagnone et al 1998b) which is a common trait in plants over-expressing the A. majus AmMYB330 and AmMYB308 (Tamagnone et al 1998a) and AtMYB4 (Jin et al 2000). On the contrary, the A. thaliana plants over-expressing ZmMYB42 present a weaker phenotype characterized only by the reduction of the growth rate and by leaf curvature, whereas the over-expression of ZmMYB8 does not lead to visible alterations.…”

Section: Discussionmentioning

confidence: 86%

Down-regulation of the maize and Arabidopsis thaliana caffeic acid O-methyl-transferase genes by two new maize R2R3-MYB transcription factors

et al. 2006

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The maize (Zea mays L.) caffeic acid O-methyl-transferase (COMT) is a key enzyme in the biosynthesis of lignin. In this work we have characterized the involvement of COMT in the lignification process through the study of the molecular mechanisms involved in its regulation. The examination of the maize COMT gene promoter revealed a putative ACIII box, typically recognized by R2R3-MYB transcription factors. We used the sequence of known R2R3-MYB factors to isolate five maize R2R3-MYB factors (ZmMYB2, ZmMYB8, ZmMYB31, ZmMYB39, and ZmMYB42) and study their possible roles as regulators of the maize COMT gene. The factors ZmMYB8, ZmMY31, and ZmMYB42 belong to the subgroup 4 of the R2R3-MYB family along with other factors associated with lignin biosynthesis repression. In addition, the induction pattern of ZmMYB31 and ZmMYB42 gene expression on wounding is that expected for repressors of the maize COMT gene. Arabidopsis thaliana plants over-expressing ZmMYB31 and ZmMYB42 down-regulate both the A. thaliana and the maize COMT genes. Furthermore, the over-expression of ZmMYB31 and ZmMYB42 also affect the expression of other genes of the lignin pathway and produces a decrease in lignin content of the transgenic plants.

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Section: Discussionmentioning

confidence: 86%

Down-regulation of the maize and Arabidopsis thaliana caffeic acid O-methyl-transferase genes by two new maize R2R3-MYB transcription factors

et al. 2006

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“…Free radicals derived from oxygen and other phenolic derivatives play important roles during plant tissue senescence activity (Tamagnone et al 1998). HCAA of spermidine in Arabidopsis and genes encoding a spermidine hydroxycinnamoyltransferase and an acyl transferase have been characterized to be involved in the formation of HCAA in the tapetum of Arabidopsis anthers and seeds (Alcázar et al 2010).…”

Section: Senescencementioning

confidence: 99%

Biosynthesis, physiology, and functions of hydroxycinnamic acid amides in plants

Macoy

Kim

Lee

et al. 2015

Plant Biotechnol Rep

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The study of hydroxycinnamic acid amides (HCAAs) which are a group of secondary metabolites has been an interesting one and has become one of the important researches at present. Accumulation of several plant amides was detected in various plants, which play important role in plant growth and development. This paper aims to review the biosynthesis, physiology, and functions of HCAA accumulation in plants during plant growth and development as well as in response to senescence and drought stress. HCAAs are secondary metabolites derived from phenylalanine and tyrosine pathway. Phenylalanine ammonia lyase (PAL) and 4-coumarate CoA ligase (4CL) hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl) transferase (THT) and tyrosine decarboxylase (TyDC) are essential enzymes for HCAA biosynthesis. HCAAs contribute to many developmental processes as well as plant responses against biotic and abiotic stress responses. However, there is a need to specifically investigate the role of many HCAAs in view of plant molecular biology since it is still not fully conceptualized and explained at present.

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“…The vir regulon is coordinately induced in response to host phenolic compounds in combination with monosaccharides and extracellular acidity in the range of pH 5-5.5. Vir inducers are not detected or detected at very low levels in uninjured plants, but their amounts increase rapidly in wounded plant cells (Stachel et al 1985a;Teutonico et al 1991;Tamagnone et al 1998;Chevrot et al 2006). These chemical stimuli are detected by the transmembrane twocomponent sensor kinase VirA (Lee et al 1995;Gelvin 2006).…”

Section: Introductionmentioning

confidence: 96%

O-methyltransferase(s)-suppressed plants produce lower amounts of phenolic vir inducers and are less susceptible to Agrobacterium tumefaciens infection

Maury

Delaunay

Mesnard

et al. 2010

Planta

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The first step of Agrobacterium tumefaciens/plant interaction corresponds to the activation of a transduction pathway of the bacterium by plant exudate. Phenolic compounds rapidly secreted by wounded plant cells induce the expression of bacterial virulence (vir) genes; however, little is known about their biosynthesis in plant. Here we show that inoculation of an Agrobacterium tumefaciens virulent strain on orthodiphenol-O-methyltransferases-suppressed tobacco plants leads to significantly smaller tumors compared to control plants. These transgenic plants are inhibited for caffeic acid O-methyltransferase class I or II (OMT; EC 2.1.1.6) and/or caffeoyl-coenzyme A O-methyltransferase (CCoAOMT; EC 2.1.1.104) that are involved in monolignol biosynthesis. The significant decrease of tumor size could be suppressed by the pre-activation of bacterial virulence, before inoculation, using acetosyringone a known vir inducer. Total soluble phenolic amounts and cell wall composition analyzed by FT-IR analysis did not show significant differences between transgenic and control plants. The potential of phenolic extracts from control and OMT-suppressed plants to induce virulence was evaluated using an Agrobacterium tumefaciens reporter strain carrying a vir::LacZ gene fusion plasmid. Lower vir-inducing activities were recorded for plants that show inhibition to caffeic acid O-methyltransferase activity. HPLC analysis confirmed that the levels of several phenolic compounds were differently affected by wounding and/or by bacterial inoculation. Statistical correlations were established between tumor sizes, vir-inducing activities, O-methyltransferases proteins accumulations and the levels of various soluble phenolic compounds such as acetosyringone. These results demonstrate the role of the O-methyltransferases of the phenylpropanoid pathway in the early production of soluble Agrobacterium tumefaciens vir inducers.

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Inhibition of Phenolic Acid Metabolism Results in Precocious Cell Death and Altered Cell Morphology in Leaves of Transgenic Tobacco Plants

Cited by 31 publications

References 0 publications

Down-regulation of the maize and Arabidopsis thaliana caffeic acid O-methyl-transferase genes by two new maize R2R3-MYB transcription factors

Down-regulation of the maize and Arabidopsis thaliana caffeic acid O-methyl-transferase genes by two new maize R2R3-MYB transcription factors

Biosynthesis, physiology, and functions of hydroxycinnamic acid amides in plants

O-methyltransferase(s)-suppressed plants produce lower amounts of phenolic vir inducers and are less susceptible to Agrobacterium tumefaciens infection

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