Inhibition of pathogenicSalmonellaenteritidisgrowth mediated byEscherichia colimicrocin J25 producing strains

Portrait, Véronique; Gendron-Gaillard, Sandrine; Cottenceau, Gilles; Pons, Anne-Marie

doi:10.1139/w99-106

Cited by 54 publications

(41 citation statements)

References 16 publications

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“…coli DSM 6601 was one of the first strains isolated with the idea of using the strain as a competitor to inhibit growth of pathogenic bacteria in the gut. Since then, additional strains with suppressing activities against pathogenic strains have been isolated in various laboratories (Portrait et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN

et al. 2003

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The colicin G producer Escherichia coli CA46, the colicin H producer E. coli CA58 and E. coli Nissle 1917 (DSM 6601) were shown to produce microcin H47 and the newly described microcin M. Both microcins were exported like colicin V by an RND-type export system, including TolC. The gene cluster encoding microcins H47 and M in strains CA46 and CA58 is nearly identical to that in strain DSM 6601, except that two additional genes are included. A Fur box identified in front of the microcin-encoding genes explained the observed iron regulation of microcin production. The catecholate siderophore receptors Fiu, Cir and FepA from E. coli and IroN, Cir and FepA from Salmonella were identified as receptors for microcins M, H47 and E492. IroN takes up the glucose-containing catecholate siderophore salmochelin, whose synthesis is encoded in the iro gene cluster found in Salmonella and certain, often uropathogenic, E. coli strains. A gene in this iro cluster, iroB, which encodes a putative glycosyltransferase, was also found in the microcin H47/M and microcin E492 gene clusters. These microcins could aid the producing strain in competing against enterobacteria that utilize catecholate siderophores.

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Section: Discussionmentioning

confidence: 99%

The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN

et al. 2003

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“…The strain which showed the maximum zone of clearance was chosen for further study. The presence of zone of clearance on agar plates was used as an indicator of bioactive potential of the strain (Portrait et al, 1999).…”

Section: Screening Of Bacterial Isolates For Antagonistic Activity Agmentioning

confidence: 99%

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

Anjhana¹,

Sasikala²

2017

Int. J. Adv. Res. Biol. Sci.

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In the present work marine sediment samples were collected from Thengapattanam estuary on the south west coast of India for the isolation and screening of bacteria for biocidal potency. The density of the bacterial load in the sediment samples was ranged from 5.43x10 4 to 2.8x10 6 CFU/g. Screening of bacterial isolates for antagonistic activity revealed that the isolate MS21 had the highest zone of clearance among against all the 10 plant fungal pathogens tested. Among 10 fungal pathogens, the highest antagonistic activity was observed against Gleosporium gleosporioide (36mm) and the lowest against Colletotrichum lamella (11mm). The potential isolate was identified as Bacillus subtilis using biochemical methods and it was designated as Bacillus subtilis MS21. Effect of various physicochemical parameters on growth of B. subtilis MS21 showed that 36 hrs of incubation period, 150 rpm agitation, pH-8.0, temperature-35 o C, salinity-1.0%, 2% sucrose as carbon source and 1% beef extract as nitrogen source were found to be the ideal conditions for maximum growth. Maximum growth of 1.92 OD was observed in mass scale culture with the optimized ideal conditions in the shake flask.

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“…Colicin immunity is specific, but in some cases, other mechanisms are also involved, such as pumping microcin out of the cells. The bactericidal spectrum of activity was found to be restricted to Enterobacteriaceae and specifically directed against Escherichia (333) and Salmonella (320,397) species. The microcin inserts into the inner membrane, whereupon the potential becomes destabilized due to pore formation that leads to depolarization and permeabilization of the E. coli cytoplasmic membrane (25,84,328).…”

Section: Barrier Effect Against Pathogensmentioning

confidence: 99%

The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

2006

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SUMMARY The intestinal tract is a complex ecosystem that combines resident microbiota and the cells of various phenotypes with complex metabolic activities that line the epithelial wall. The intestinal cells that make up the epithelium provide physical and chemical barriers that protect the host against the unwanted intrusion of microorganisms that hijack the cellular molecules and signaling pathways of the host and become pathogenic. Some of the organisms making up the intestinal microbiota also have microbicidal effects that contribute to the barrier against enteric pathogens. This review describes the two cell lineages present in the intestinal epithelium: the goblet cells and the Paneth cells, both of which play a pivotal role in the first line of enteric defense by producing mucus and antimicrobial peptides, respectively. We also analyze recent insights into the intestinal microbiota and the mechanisms by which some resident species act as a barrier to enteric pathogens. Moreover, this review examines whether the cells producing mucins or antimicrobial peptides and the resident microbiota act in partnership and whether they function individually and/or synergistically to provide the host with an effective front line of defense against harmful enteric pathogens.

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Inhibition of pathogenicSalmonellaenteritidisgrowth mediated byEscherichia colimicrocin J25 producing strains

Cited by 54 publications

References 16 publications

The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN

The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN

Isolation, screening and growth optimization of antagonistic Bacillus subtilis MS21 from Thengapattanam estuary against plant fungal pathogens

The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

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