Inhibition of ovulation, steroidogenesis and collagenolytic activity in rabbits by sulpiride-induced hyperprolactinaemia

Lin, Kuo‐Cherng; Kawamura, N.; Okamura, Hitoshi; Mori, Takahide

doi:10.1530/jrf.0.0830611

Cited by 38 publications

(23 citation statements)

References 23 publications

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“…Ten rats were not treated and served as the normal control. Hyperprolactinemia was induced in 50 rats using metoclopramide dihydrochloride (50 mg/kg, tid×10 d, ip) [16] . The hyperprolactinemia SD rats were then treated daily with saline (vehicle control), bromocriptine (1 mg/kg) or different doses of casticin (10,20, and 40 mg/kg, ip) for 1 week.…”

Section: Animal Model and Drug Treatmentmentioning

confidence: 99%

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Casticin, a flavonoid isolated from Vitex rotundifolia, inhibits prolactin release in vivo and in vitro

Zhang

Zheng

et al. 2010

Acta Pharmacol Sin

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Aim: To investigate the anti-hyperprolactinemia activity of casticin, a flavonoid isolated from Vitex rotundifolia, and elucidate its molecular mechanism. Methods: Hyperprolactinemia (MIHP) was induced by administration of metoclopramide dihydrochloride (50 mg/kg, tid, ip, for 10 d) in SD rats and the primary pituitary cells were prepared from the pituitary glands of the SD rats. Prolactin concentrations were measured using a radioimmunoassay. Cell viability was measured using an MTT assay. The mRNA expression of estrogen receptor alpha and beta in rat pituitary cells was measured using semi-quantitative RT-PCR analysis. Results: The level of serum prolactin in the MIHP model group was 2.1 fold higher than that in the untreated control group (P<0.01). Casticin (10, 20, and 40 mg/kg, ip, for 7 d) reduced serum prolactin levels by 33.9%, 54.3%, and 64.7%, respectively (P<0.01). The positive control drug bromocriptine 1 mg/kg decreased the serum prolactin concentration in MIHP rats by 44.9%. 17β-Estradiol (E2) significantly increased the proliferation of pituitary cells and casticin (1 and 10 μmol/L) markedly inhibited E2-induced pituitary cell proliferation by 27.7% and 42.1%, respectively. Stimulation of pituitary cells with E2 increased prolactin secretion into the cell culture supernatants, and casticin (0.1, 1, and 10 μmol/L) significantly inhibited the prolactin release stimulated by E2 in a concentration-dependent manner. Casticin (1 and 10 μmol/L) significantly inhibited ERα mRNA expression in pituitary cells stimulated with E2 (P<0.01) but increased ERβ mRNA expression at a concentration of 10 μmol/L (P<0.01). However, casticin had no effects on proliferation and prolectin release of the unstimulated primary pituitary cells in vitro. Conclusion: Casticin inhibited the release of prolactin from pituitary cells of SD rats stimulated with E2 in vivo and in vitro. These effects might be related with inhibiting the ERα mRNA expression and increasing the ERβ mRNA expression.

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Section: Animal Model and Drug Treatmentmentioning

confidence: 99%

“…Blood samples were collected from the orbit vein without anesthesia. The sera were obtained by centrifugation at 2500×g for 15 min and were stored at -20 °C until the prolactin assay was performed [16,17] .…”

Section: Animal Model and Drug Treatmentmentioning

confidence: 99%

Casticin, a flavonoid isolated from Vitex rotundifolia, inhibits prolactin release in vivo and in vitro

Zhang

Zheng

et al. 2010

Acta Pharmacol Sin

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“…Hyperprolactinemic rats were generated as previously described with slight modifications [11]. Metoclopramide dihydrochloride (50 mg/kg) was subcutaneously injected into the back of rats.…”

Section: Preparation Of Hyperprolactinemic Rats and Fple Treatmentmentioning

confidence: 99%

Anti-hyperprolactinemic effect of Ficus pumila Linn extract in rats

He¹,

Fan²,

Liu³

et al. 2016

Trop. J. Pharm Res

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“…The hyperprolactinemia rat model was performed as previously described with a little modification (Lin et al, 1988).…”

Section: Preparation Of Model Ratsmentioning

confidence: 99%

Effect of Chinese medical herbs-Huiru Yizeng Yihao on hyperprolactinemia and hyperplasia of mammary gland in mice

Wang¹,

Chen²,

Ma³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

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The study investigated the pharmacodynamism and mechanism of Chinese medicinal formula-Huiru Yizeng Yihao (NO.1 HRYZ) on the model rats of hyperpro-lactinemia and the model rats of hyperplasia of mammary gland (HMG), and studied the internal connection between hyperprolactinemia and HMG.. The hyperprolactinemia rat models were established by injecting metoclopramide dihydrochloride in the back of rats. The model rat of HMG was prepared by injecting estradiol in the thigh muscle of the rats and progesterone consecutively, while the tails of rats were clipped with tongs. Rats were treated with either NO.1 HRYZ or positive control drugs for four weeks. The concentrations of sex hormone in rat serum were examined using ELISA kits, and the morphology of mammary gland tissue in all group rats was observed with microscope. NO.1 HRYZ significantly decreased prolactin (PRL) and increased estradiol (E 2 ), progesterone (P), follicle stimulating hormone (FSH), luteinizing hormone (LH) concentrations of hyperprolactinemia rats. It decreased E 2, PRL, FSH, gonadotropin-releasing hormone (GnRH), 5-hydroxytryptamine (5-HT) and increased P concentrations of HMG rat. It also eliminated hyperplasia of lobules and gland alveolus compared with the model group. Treatment with NO.1 HRYZ could significantly regulate the sex hormone disorder of hyperprolactinemia and HMG rat models, and could eliminate the formation of HMG. Hyperprolactinemia was closely correlated with HMG, and hyperprolactinemia promoted the formation of HMG.

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Inhibition of ovulation, steroidogenesis and collagenolytic activity in rabbits by sulpiride-induced hyperprolactinaemia

Cited by 38 publications

References 23 publications

Casticin, a flavonoid isolated from Vitex rotundifolia, inhibits prolactin release in vivo and in vitro

Casticin, a flavonoid isolated from Vitex rotundifolia, inhibits prolactin release in vivo and in vitro

Anti-hyperprolactinemic effect of Ficus pumila Linn extract in rats

Effect of Chinese medical herbs-Huiru Yizeng Yihao on hyperprolactinemia and hyperplasia of mammary gland in mice

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