Inhibition of ovarian cancer cell proliferation by Pien Tze Huang via the AKT-mTOR pathway

He, Fan; Wu, Haoran; Cai, Mengli; Li, Chang‐Peng; Zhang, Xin; Wan, Quan; Tang, Shifu; Cheng, Jianding

doi:10.3892/ol.2014.1989

Cited by 19 publications

(9 citation statements)

References 26 publications

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“…TCMs have received recent interest in this regard, as they have relatively few side effects and have been used for thousands of years as alternative remedies for a number of diseases, including cancer ( 7 , 8 ). The well-known TCM formula PZH has long been used in China to treat a number of cancers ( 45 , 51 , 52 ).…”

Section: Discussionmentioning

confidence: 99%

Pien Tze Huang induces apoptosis and inhibits proliferation of 5-fluorouracil-resistant colorectal carcinoma cells via increasing miR-22 expression

Chen

Shen

Liu

et al. 2017

Experimental and Therapeutic Medicine

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The well-known traditional Chinese medicine formula Pien Tze Huang (PZH) has long been used to treat various malignancies, including colorectal cancer (CRC). It was recently reported that PZH possesses the ability to overcome multidrug resistance in CRC cells. In the present study, a 5-fluorouracil (5-FU) resistant human CRC cell line (HCT-8/5-FU) was used to further evaluate the effect of PZH on chemotherapy (chemo)-resistance and investigate the mechanisms through which this occurs. The results identified that PZH significantly reduced the viability and cell density of HCT-8/5-FU cells in a dose- and time-dependent manner (P<0.05). PZH inhibited cell survival, reduced the proportion of cells in S-phase, and suppressed the expression of pro-proliferative proteins cyclin D1 and cyclin-dependent kinase 4. In addition, PZH treatment induced nuclear condensation and fragmentation, activated caspase-9 and −3 and increased the pro-apoptotic Bcl-2-associated X protein/B-cell lymphoma 2 protein ratio. Furthermore, PZH treatment upregulated the expression of microRNA-22 (miR-22) and downregulated the expression of c-Myc (a target gene of miR-22). In conclusion, the findings from the present study suggest that PZH can overcome chemo-resistance in cancer cells, likely through increasing miR-22 expression, and by reversing the imbalance between levels of proliferation and apoptosis.

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Section: Discussionmentioning

confidence: 99%

Pien Tze Huang induces apoptosis and inhibits proliferation of 5-fluorouracil-resistant colorectal carcinoma cells via increasing miR-22 expression

Chen

Shen

Liu

et al. 2017

Experimental and Therapeutic Medicine

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“…Proliferation and apoptosis of cells are two critical factors that determine organism development and tissue homeostasis [ 86 , 87 ]. Precisely, it is the unrestricted proliferation and suppressed apoptosis that make cancer cells grow frantically and malignantly.…”

Section: Tcm Inhibits the Development Of The “Seed”mentioning

confidence: 99%

“…Although the mechanisms are not very clear, increasing data has shown that they maybe relate with above-mentioned regulating biomarkers to inhibit proliferation and induce apoptosis of cancer cells. The confirmed mechanisms of herb compounds and monomers acting common cancers are listed in Table 2 [ 86 , 88 , 91 , 92 , 95 , 97 , 99 – 103 ].…”

Section: Tcm Inhibits the Development Of The “Seed”mentioning

confidence: 99%

Synergistic Effect and Molecular Mechanisms of Traditional Chinese Medicine on Regulating Tumor Microenvironment and Cancer Cells

Song

Guo

et al. 2016

BioMed Research International

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The interaction of tumor cells with the microenvironment is like a relationship between the “seeds” and “soil,” which is a hotspot in recent cancer research. Targeting at tumor microenvironment as well as tumor cells has become a new strategy for cancer treatment. Conventional cancer treatments mostly focused on single targets or single mechanism (the seeds or part of the soil); few researches intervened in the whole tumor microenvironment and achieved ideal therapeutic effect as expected. Traditional Chinese medicine displays a broad range of biological effects, and increasing evidence has shown that it may relate with synergistic effect on regulating tumor microenvironment and cancer cells. Based on literature review and our previous studies, we summarize the synergistic effect and the molecular mechanisms of traditional Chinese medicine on regulating tumor microenvironment and cancer cells.

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“…In CRC patients, the synthesis of UDP‐N‐acetyl‐D‐glucosamine (UDP‐GlcNAc) is increased, which is the substrate for OGT for O‐GlcNAcylation of target proteins and O‐GlcNAcylation of certain proteins may be a key factor in tumorigenesis . In addition, abnormal activation of AKT/mTOR signalling is the key factor of CRC, which leads to tumour growth and metastasis . Previous studies have shown that DDX5 can transcriptionally co‐activate AKT signaling pathway in CRC to regulate cancer progression .…”

Section: Introductionmentioning

confidence: 99%

O‐GlcNAcylation promotes colorectal cancer progression by regulating protein stability and potential catcinogenic function of DDX5

Jiang

Han

et al. 2018

J Cellular Molecular Medi

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The RNA helicase p68 (DDX5), a key player in RNA metabolism, belongs to the DEAD box family and is involved in the development of colorectal cancer. Here, we found both DDX5 and O‐GlcNAcylation are up‐regulated in colorectal cancer. In addition, DDX5 protein level is significantly positively correlated with the expression of O‐GlcNAcylation. Although it was known DDX5 protein could be regulated by post‐translational modification (PTM), how O‐GlcNAcylation modification regulated of DDX5 remains unclear. Here we show that DDX5 interacts directly with OGT in the SW480 cell line, which is the only known enzyme that catalyses O‐GlcNAcylation in humans. Meanwhile, O‐GlcNAcylation could promote DDX5 protein stability. The OGT‐DDX5 axis affects colorectal cancer progression mainly by regulating activation of the AKT/mTOR signalling pathway. Taken together, these results indicated that OGT‐mediated O‐GlcNAcylation stabilizes DDX5, promoting activation of the AKT/mTOR signalling pathway, thus accelerating colorectal cancer progression. This study not only reveals the novel functional of O‐GlcNAcylation in regulating DDX5, but also reveals the carcinogenic effect of the OGT‐DDX5 axis in colorectal cancer.

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Inhibition of ovarian cancer cell proliferation by Pien Tze Huang via the AKT-mTOR pathway

Cited by 19 publications

References 26 publications

Pien Tze Huang induces apoptosis and inhibits proliferation of 5-fluorouracil-resistant colorectal carcinoma cells via increasing miR-22 expression

Pien Tze Huang induces apoptosis and inhibits proliferation of 5-fluorouracil-resistant colorectal carcinoma cells via increasing miR-22 expression

Synergistic Effect and Molecular Mechanisms of Traditional Chinese Medicine on Regulating Tumor Microenvironment and Cancer Cells

O‐GlcNAcylation promotes colorectal cancer progression by regulating protein stability and potential catcinogenic function of DDX5

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